scholarly journals Human EZF, a Krüppel-like Zinc Finger Protein, Is Expressed in Vascular Endothelial Cells and Contains Transcriptional Activation and Repression Domains

1998 ◽  
Vol 273 (2) ◽  
pp. 1026-1031 ◽  
Author(s):  
Shaw-Fang Yet ◽  
Megan M. McA’Nulty ◽  
Sara C. Folta ◽  
Hsueh-Wei Yen ◽  
Masao Yoshizumi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Zinc Finger ◽  
Transcriptional Activation ◽  
Zinc Finger Protein ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Finger Protein

Single zinc-finger extension: enhancing transcriptional activity and specificity of three-zinc-finger proteins

2005 ◽  
Vol 386 (2) ◽  
pp. 95-99 ◽  
Author(s):  
Alexander E.F. Smith ◽  
Farzin Farzaneh ◽  
Kevin G. Ford
Keyword(s):  
Transcription Factors ◽  
Fusion Protein ◽  
Zinc Finger ◽  
Protein Interactions ◽  
Transcriptional Activation ◽  
Zinc Finger Protein ◽  
Zinc Finger Proteins ◽  
Finger Protein ◽  
Finger Extension ◽  
Vp16 Fusion

AbstractIn order to demonstrate that an existing zinc-finger protein can be simply modified to enhance DNA binding and sequence discrimination in both episomal and chromatin contexts using existing zinc-finger DNA recognition code data, and without recourse to phage display and selection strategies, we have examined the consequences of a single zinc-finger extension to a synthetic three-zinc-finger VP16 fusion protein, on transcriptional activation from model target promoters harbouring the zinc-finger binding sequences. We report a nearly 10-fold enhanced transcriptional activation by the four-zinc-finger VP16 fusion protein relative to the progenitor three-finger VP16 protein in transient assays and a greater than five-fold enhancement in stable reporter-gene expression assays. A marked decrease in transcriptional activation was evident for the four-zinc-finger derivative from mutated regulatory regions compared to the progenitor protein, as a result of recognition site-size extension. This discriminatory effect was shown to be protein concentration-dependent. These observations suggest that four-zinc-finger proteins are stable functional motifs that can be a significant improvement over the progenitor three-zinc-finger protein, both in terms of specificity and the ability to target transcriptional function to promoters, and that single zinc-finger extension can therefore have a significant impact on DNA zinc-finger protein interactions. This is a simple route for modifying or enhancing the binding properties of existing synthetic zinc-finger-based transcription factors and may be particularly suited for the modification of endogenous zinc-finger transcription factors for promoter biasing applications.

scholarly journals Broadly active zinc finger protein-guided transcriptional activation of HIV-1

2021 ◽  
Vol 20 ◽  
pp. 18-29
Author(s):  
Tristan A. Scott ◽  
Denis O’Meally ◽  
Nicole Anne Grepo ◽  
Citradewi Soemardy ◽  
Daniel C. Lazar ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Transcriptional Activation ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Hiv 1

The zinc finger protein A20 protects endothelial cells from burns serum injury

Burns ◽  
2004 ◽  
Vol 30 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Chu-hong Zhu ◽  
Da-jun Ying ◽  
Jian-hong Mi ◽  
Wei Zhang ◽  
Shi-wu Dong ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein

scholarly journals Zinc finger protein A20 overexpression inhibits monocyte homing and protects endothelial cells from injury induced by high glucose

2011 ◽  
Vol 10 (2) ◽  
pp. 1050-1059 ◽  
Author(s):  
C.L. Hou ◽  
W. Zhang ◽  
Y. Wei ◽  
J.H. Mi ◽  
L. Li ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Zinc Finger ◽  
High Glucose ◽  
Zinc Finger Protein ◽  
Finger Protein

scholarly journals Transcriptional activation ofBrassica napusβ-ketoacyl-ACP synthase II with an engineered zinc finger protein transcription factor

2012 ◽  
Vol 10 (7) ◽  
pp. 783-791 ◽  
Author(s):  
Manju Gupta ◽  
Russell C. DeKelver ◽  
Asha Palta ◽  
Carla Clifford ◽  
Sunita Gopalan ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Zinc Finger ◽  
Transcriptional Activation ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Protein Transcription
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