The p75 Neurotrophin Receptor Interacts with Multiple MAGE Proteins

The p75 neurotrophin receptor has been implicated in diverse aspects of neurotrophin signaling, but the mechanisms by which its effects are mediated are not well understood. Here we identify two MAGE proteins, necdin and MAGE-H1, as interactors for the intracellular domain of p75 and show that the interaction is enhanced by ligand stimulation. PC12 cells transfected with necdin or MAGE-H1 exhibit accelerated differentiation in response to nerve growth factor. Expression of these two MAGE proteins is predominantly cytoplasmic in PC12 cells, and necdin was found to be capable of homodimerization, suggesting that it may act as a cytoplasmic adaptor to recruit a signaling complex to p75. These findings indicate that diverse MAGE family members can interact with the p75 receptor and highlight type II MAGE proteins as a potential family of interactors for signaling proteins containing type II death domains.

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Growth arrest of PC12 cells by nerve growth factor is dependent on the phosphatidylinositol 3-kinase/Akt pathway via p75 neurotrophin receptor

Journal of Neuroscience Research ◽

10.1002/jnr.10564 ◽

2003 ◽

Vol 72 (2) ◽

pp. 211-217 ◽

Cited By ~ 13

Author(s):

Hisanori Ito ◽

Hiroshi Nomoto ◽

Shoei Furukawa

Keyword(s):

Nerve Growth Factor ◽

Growth Factor ◽

Pc12 Cells ◽

Growth Arrest ◽

Neurotrophin Receptor ◽

Akt Pathway ◽

P75 Neurotrophin Receptor ◽

Phosphatidylinositol 3 Kinase ◽

Nerve Growth ◽

Phosphatidylinositol 3

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Suppression of p75 Neurotrophin Receptor Surface Expression with Intrabodies Influences Bcl-xL mRNA Expression and Neurite Outgrowth in PC12 Cells

PLoS ONE ◽

10.1371/journal.pone.0030684 ◽

2012 ◽

Vol 7 (1) ◽

pp. e30684 ◽

Cited By ~ 16

Author(s):

Congcong Zhang ◽

Saskia Helmsing ◽

Marta Zagrebelsky ◽

Thomas Schirrmann ◽

Andrea L. J. Marschall ◽

...

Keyword(s):

Mrna Expression ◽

Neurite Outgrowth ◽

Pc12 Cells ◽

Surface Expression ◽

Neurotrophin Receptor ◽

P75 Neurotrophin Receptor ◽

Receptor Surface Expression

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p75 neurotrophin receptor is required for constitutive and NGF-induced survival signalling in PC12 cells and rat hippocampal neurones

Journal of Neurochemistry ◽

10.1046/j.1471-4159.2002.00841.x ◽

2002 ◽

Vol 81 (3) ◽

pp. 594-605 ◽

Cited By ~ 48

Author(s):

Nguyen Truc Bui ◽

Hans-Georg König ◽

Carsten Culmsee ◽

Elke Bauerbach ◽

Monika Poppe ◽

...

Keyword(s):

Pc12 Cells ◽

Neurotrophin Receptor ◽

P75 Neurotrophin Receptor

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Retrograde apoptotic signaling by the p75 neurotrophin receptor

Neuronal Signaling ◽

10.1042/ns20160007 ◽

2017 ◽

Vol 1 (1) ◽

Cited By ~ 4

Author(s):

Amrita Pathak ◽

Bruce D. Carter

Keyword(s):

System Development ◽

Nervous System Development ◽

Neurotrophin Receptor ◽

P75 Neurotrophin Receptor ◽

Cell Soma ◽

Apoptotic Signaling ◽

Survival Signaling ◽

P75 Receptor ◽

Apoptotic Signal

Neurotrophins are target-derived factors necessary for mammalian nervous system development and maintenance. They are typically produced by neuronal target tissues and interact with their receptors at axonal endings. Therefore, locally generated neurotrophin signals must be conveyed from the axon back to the cell soma. Retrograde survival signaling by neurotrophin binding to Trk receptors has been extensively studied. However, neurotrophins also bind to the p75 receptor, which can induce apoptosis in a variety of contexts. Selective activation of p75 at distal axon ends has been shown to generate a retrograde apoptotic signal, although the mechanisms involved are poorly understood. The present review summarizes the available evidence for retrograde proapoptotic signaling in general and the role of the p75 receptor in particular, with discussion of unanswered questions in the field. In-depth knowledge of the mechanisms of retrograde apoptotic signaling is essential for understanding the etiology of neurodegeneration in many diseases and injuries.

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