scholarly journals Inhibition of Lens Fiber Cell Morphogenesis by Expression of a Mutant SV40 Large T Antigen That Binds CREB-binding Protein/p300 but Not pRb

2004 ◽  
Vol 279 (17) ◽  
pp. 17667-17673 ◽  
Author(s):  
Qin Chen ◽  
Dongcai Liang ◽  
Larry D. Fromm ◽  
Paul A. Overbeek
Keyword(s):  
Binding Protein ◽  
T Antigen ◽  
Fiber Cell ◽  
Creb Binding Protein ◽  
Lens Fiber ◽  
Large T Antigen ◽  
Cell Morphogenesis ◽  
Sv40 Large T Antigen ◽  
Lens Fiber Cell

scholarly journals Targeting of p300/CREB Binding Protein Coactivators by Simian Virus 40 Is Mediated through p53

2006 ◽  
Vol 80 (9) ◽  
pp. 4292-4303 ◽  
Author(s):  
Darrell R. Borger ◽  
James A. DeCaprio
Keyword(s):  
Binding Protein ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Dependent Manner ◽  
Creb Binding Protein ◽  
Large T Antigen ◽  
P300 Binding ◽  
Essential Function ◽  
Secondary Function

ABSTRACT The primary transforming functions of simian virus 40 large T antigen (SV40 LT) are conferred primarily through the binding and inactivation of p53 and the retinoblastoma family members. Normal p53 function requires an association with the CREB binding protein (CBP)/p300 coactivators, and a ternary complex containing SV40 LT, p53, and CBP/p300 has been identified previously. In this report, we have evaluated a secondary function of p53 bound to the SV40 LT complex in mediating the binding of human CBP/p300. We demonstrate that p53 associated with SV40 LT was posttranslationally modified in a manner consistent with the binding of CBP/p300. Furthermore, expression of SV40 LT induced the proportion of p53 phosphorylated on S15. An essential function for p53 in bridging the interaction between SV40 LT and CBP/p300 was identified through the reconstitution of the SV40 LT-CBP/p300 complex upon p53 reexpression in p53-null cells. In addition, the SV40 LT-CBP/p300 complex was disrupted through RNA interference-mediated depletion of endogenous p53. We also demonstrate that SV40 LT was acetylated in a p300- and p53-dependent manner, at least in part through the CH3 domain of p300. Therefore, the binding of p53 serves to modify SV40 LT by targeting CBP and p300 binding to direct the acetylation of SV40 LT.

scholarly journals Fibroblast growth factor receptor signaling is essential for lens fiber cell differentiation

2008 ◽  
Vol 318 (2) ◽  
pp. 276-288 ◽  
Author(s):  
Haotian Zhao ◽  
Tianyu Yang ◽  
Bhavani P. Madakashira ◽  
Cornelius A. Thiels ◽  
Chad A. Bechtle ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor Receptor ◽  
Growth Factor Receptor ◽  
Fiber Cell ◽  
Fibroblast Growth ◽  
Lens Fiber ◽  
Lens Fiber Cell ◽  
Growth Factor Receptor Signaling

Retroviral transduction of human periodontal cells with a temperature-sensitive SV40 large T antigen

1999 ◽  
Vol 44 (10) ◽  
pp. 823-834 ◽  
Author(s):  
M.H. Parkar ◽  
L. Kuru ◽  
M. O’Hare ◽  
H.N. Newman ◽  
F. Hughes ◽  
...  
Keyword(s):  
T Antigen ◽  
Temperature Sensitive ◽  
Large T Antigen ◽  
Sv40 Large T Antigen ◽  
Retroviral Transduction

SV40 small t antigen enhances the transformation activity of limiting concentrations of SV40 large T antigen

Cell ◽  
1987 ◽  
Vol 48 (2) ◽  
pp. 321-330 ◽  
Author(s):  
Ilan Bikel ◽  
Ximena Montano ◽  
Mounzer E. Agha ◽  
Myles Brown ◽  
Melissa McCormack ◽  
...  
Keyword(s):  
T Antigen ◽  
Large T Antigen ◽  
Sv40 Large T Antigen ◽  
Transformation Activity ◽  
Small T Antigen ◽  
Sv40 Small T

Recombinant retroviruses encoding simian virus 40 large T antigen and polyomavirus large and middle T antigens

1986 ◽  
Vol 6 (4) ◽  
pp. 1204-1217
Author(s):  
P S Jat ◽  
C L Cepko ◽  
R C Mulligan ◽  
P A Sharp
Keyword(s):  
Cell Lines ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Vector System ◽  
Large T Antigen ◽  
T Antigens ◽  
Sv40 Large T Antigen ◽  
Polyomavirus Middle T Antigen ◽  
Middle T Antigen

We used a murine retrovirus shuttle vector system to construct recombinants capable of constitutively expressing the simian virus 40 (SV40) large T antigen and the polyomavirus large and middle T antigens as well as resistance to G418. Subsequently, these recombinants were used to generate cell lines that produced defective helper-free retroviruses carrying each of the viral oncogenes. These recombinant retroviruses were used to analyze the role of the viral genes in transformation of rat F111 cells. Expression of the polyomavirus middle T antigen alone resulted in cell lines that were highly tumorigenic, whereas expression of the polyomavirus large T resulted in cell lines that were highly tumorigenic, whereas expression of the polyomavirus large T resulted in cell lines that were unaltered by the criteria of morphology, anchorage-independent growth, and tumorigenicity. More surprisingly, SV40 large T-expressing cell lines were not tumorigenic despite the fact that they contained elevated levels of cellular p53 and had a high plating efficiency in soft agar. These results suggest that the SV40 large T antigen is not an acute transforming gene like the polyomavirus middle T antigen but is similar to the establishment genes such as myc and adenovirus EIa.

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