scholarly journals Sampling From the Proteome to the Human Leukocyte Antigen-DR (HLA-DR) Ligandome ProceedsViaHigh Specificity

2016 ◽  
Vol 15 (4) ◽  
pp. 1412-1423 ◽  
Author(s):  
Geert P. M. Mommen ◽  
Fabio Marino ◽  
Hugo D. Meiring ◽  
Martien C. M. Poelen ◽  
Jacqueline A. M. van Gaans-van den Brink ◽  
...  

1983 ◽  
Vol 148 (4) ◽  
pp. 676-681 ◽  
Author(s):  
S. P. N. Singh ◽  
N. K. Mehra ◽  
H. B. Dingley ◽  
J. N. Pande ◽  
M. C. Vaidya


1998 ◽  
Vol 140 (1) ◽  
pp. 71-79 ◽  
Author(s):  
Richard J. Cherry ◽  
Keith M. Wilson ◽  
Kathy Triantafilou ◽  
Peter O'Toole ◽  
Ian E.G. Morrison ◽  
...  

The technique of single-particle fluorescence imaging was used to investigate the oligomeric state of MHC class II molecules on the surface of living cells. Cells transfected with human leukocyte antigen (HLA)–DR A and B genes were labeled at saturation with a univalent probe consisting of Fab coupled to R-phycoerythrin. Analysis of the intensities of fluorescent spots on the cell surface revealed the presence of single and double particles consistent with the simultaneous presence of HLA-DR heterodimers and dimers of dimers. The proportion of double particles was lower at 37°C than at 22°C, suggesting that the heterodimers and dimers of dimers exist in a temperature-dependent equilibrium. These results are discussed in the context of a possible role for HLA-DR dimers of dimers in T cell receptor–MHC interactions. The technique is validated by demonstrating that fluorescence imaging can distinguish between dimers and tetramers of human erythrocyte spectrin deposited from solution onto a solid substrate. The methodology will have broad applicability to investigation of the oligomeric state of immunological and other membrane-bound receptors in living cells.



2005 ◽  
Vol 66 (1) ◽  
pp. 43-55 ◽  
Author(s):  
Anthi Ranella ◽  
Simon Vassiliadis ◽  
Chrisa Mastora ◽  
Michailidou Valentina ◽  
Eva Dionyssopoulou ◽  
...  


2016 ◽  
Vol 15 (9) ◽  
pp. 3017-3029 ◽  
Author(s):  
Tina Heyder ◽  
Maxie Kohler ◽  
Nataliya K. Tarasova ◽  
Sabrina Haag ◽  
Dorothea Rutishauser ◽  
...  




AIDS ◽  
1994 ◽  
Vol 8 (12) ◽  
pp. 1731-1732 ◽  
Author(s):  
M. R. Capobianchi ◽  
C. Serra ◽  
I. Abbate ◽  
P. Marongiu ◽  
C. Castilletti ◽  
...  


1996 ◽  
Vol 219 (3) ◽  
pp. 207-210 ◽  
Author(s):  
Toshio Katsuragi ◽  
Eizo Iseki ◽  
Kenji Kosaka ◽  
Toru Nishimura ◽  
Haruhiko Akiyama ◽  
...  


2020 ◽  
Vol 129 (1) ◽  
pp. e141-e142
Author(s):  
EMANUENE GALDINO PIRES ◽  
LUANA SAMARA BALDUÍNO DE SENA ◽  
HELLEN BANDEIRA DE PONTES SANTOS ◽  
MARINA GONÇALVES DO AMARAL ◽  
MANUEL ANTÔNIO GORDÓN-NÚÑEZ ◽  
...  


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