Molecular Typing of Aspergillus ochraceus and Construction of Species Specific SCAR-Primers Based on AFLP

2003 ◽  
Vol 26 (1) ◽  
pp. 138-146 ◽  
Author(s):  
Holger Schmidt ◽  
Matthias Ehrmann ◽  
Rudi F. Vogel ◽  
Marta H. Taniwaki ◽  
Ludwig Niessen
Keyword(s):  
Molecular Typing ◽  
Aspergillus Ochraceus ◽  
Species Specific ◽  
Scar Primers

scholarly journals A New Species-Specific Typing Method for Salivarius Group Streptococci Based on the Dephospho-Coenzyme A Kinase (coaE) Gene Sequencing

2021 ◽  
Vol 11 ◽  
Author(s):  
Mohamed M. H. Abdelbary ◽  
Gerrit Wilms ◽  
Georg Conrads
Keyword(s):  
Molecular Typing ◽  
Coenzyme A ◽  
Housekeeping Gene ◽  
Human Pathogens ◽  
Gene Sequences ◽  
Viridans Streptococci ◽  
Typing Method ◽  
Streptococcal Species ◽  
Species Specific ◽  
Viridans Group Streptococci

Viridans streptococci are a group of α-hemolytic streptococcal species. They are mainly commensals, most abundant in the mouth supporting oral health. But they also include important human pathogens such as Streptococcus pneumoniae. Identification and molecular typing of viridans group streptococci are challenging, especially for members of the salivarius group. In this study, we developed a single-locus molecular typing method that is able to differentiate among the highly phylogenetically related members of the salivarius group (S. salivarius, S. vestibularis and S. thermophilus) and might support differentiation in other groups as well. This typing approach is based on the amplification and sequence analysis of the housekeeping gene dephospho-coenzyme A kinase (coaE), a gene with unrecognized taxonomic potential to date. Here, we analysed coaE gene sequences of 154 publicly available genomes and of 30 salivarius group isolates of our own collection that together belong to 20 different gram-positive bacterial (sub) species. Our results revealed that the coaE phylogeny distinguished between streptococcal and non-streptococcal genomes and that coaE gene sequences were species-specific. In contrast to MALDI-TOF MS performance, the coaE typing was able to precisely identify the phylogenetically very closely related members of the salivarius group.

Identification of Heterodera glycines using PCR with sequence characterised amplified region (SCAR) primers

Nematology ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 397-403 ◽  
Author(s):  
Maurice Moens ◽  
Yu Li ◽  
Shiqi Ou ◽  
Xuemin Liu ◽  
Deliang Peng
Keyword(s):  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Rapd Marker ◽  
False Negative ◽  
Nematode Species ◽  
Universal Primers ◽  
Negative Results ◽  
Pcr Products ◽  
Species Specific ◽  
Scar Primers

AbstractA single randomly amplified polymorphic DNA (RAPD) marker, OPA06477, species-specific to the soybean cyst nematode species, Heterodera glycines, was identified. After sequencing the RAPD-PCR products, primers of 24 nucleotides were designed to complement the terminal DNA sequence of the DNA fragments. This resulted in one pair of species-specific primers that were used to amplify the sequence characterised amplified regions (SCAR). The developed sets of SCAR primers were successfully used in straightforward, fast and reliable PCR assays to identify H. glycines. The SCAR markers can be amplified from DNA from single second-stage juveniles and females. This is the first time SCAR primers have been combined with universal primers D2A and D3B in order to avoid false negative results.

Detection of Antifungal Properties in Lactobacillus paracasei subsp. paracasei SM20, SM29, and SM63 and Molecular Typing of the Strains

2005 ◽  
Vol 68 (1) ◽  
pp. 111-119 ◽  
Author(s):  
SUSANNE MIESCHER SCHWENNINGER ◽  
UELI VON AH ◽  
BRIGITTE NIEDERER ◽  
MICHAEL TEUBER ◽  
LEO MEILE
Keyword(s):  
Molecular Typing ◽  
Raw Milk ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
Candida Spp ◽  
Food And Feed ◽  
Carbohydrate Fermentation ◽  
Typing Methods ◽  
Raw Milk Cheese ◽  
Species Specific

Lactobacilli isolated from different food and feed samples such as raw milk, cheese, yoghurt, olives, sour dough, as well as corn and grass silage, were screened for their antifungal activities. Out of 1,424 isolates tested, 82 were shown to be inhibitory to different yeasts (Candida spp. and Zygosaccharomyces bailii) and a Penicillium sp., which were previously isolated from spoiled yoghurt and fruits. Carbohydrate fermentation patterns suggested that a substantial portion, 25%, belonged to the Lactobacillus casei group, including L. casei, L. paracasei, and L. rhamnosus. The isolates SM20 (DSM14514), SM29 (DSM14515), and SM63 (DSM14516) were classified by PCR using species-specific primers to target the corresponding type strains (L. casei, L. paracasei, and L. rhamnosus) as controls. Further molecular typing methods such as randomly amplified polymorphic DNA, pulsed-field gel electrophoresis, and sequencing analysis of the 16S rRNA gene allowed classifying strains SM20, SM29, and SM63 as L. paracasei subsp. paracasei in accordance with the new reclassification of the L. casei group proposed by Collins et al. (Int. J. Syst. Bacteriol. 39:105–108).

Electron Microscopy in the Study of Natural Phytoplankton Assemblages

1985 ◽  
Vol 43 ◽  
pp. 620-623
Author(s):  
Linda Sicko-Goad
Keyword(s):  
Electron Microscopy ◽  
Aquatic Environment ◽  
Size Range ◽  
Physical Parameters ◽  
Specific Information ◽  
Phytoplankton Assemblages ◽  
Phytoplankton Productivity ◽  
Ecosystem Effects ◽  
Time Of Year ◽  
Species Specific

Although the use of electron microscopy and its varied methodologies is not usually associated with ecological studies, the types of species specific information that can be generated by these techniques are often quite useful in predicting long-term ecosystem effects. The utility of these techniques is especially apparent when one considers both the size range of particles found in the aquatic environment and the complexity of the phytoplankton assemblages.The size range and character of organisms found in the aquatic environment are dependent upon a variety of physical parameters that include sampling depth, location, and time of year. In the winter months, all the Laurentian Great Lakes are uniformly mixed and homothermous in the range of 1.1 to 1.7°C. During this time phytoplankton productivity is quite low.

64: Rapid, Species-Specific Detection of Uropathogens from Clinical Urine Specimens Using an Electrochemical Microsensor Array

2005 ◽  
Vol 173 (4S) ◽  
pp. 18-18
Author(s):  
Joseph C. Liao ◽  
Mitra Mastali ◽  
David A. Haake ◽  
Bernard M. Churchill
Keyword(s):  
Specific Detection ◽  
Species Specific ◽  
Urine Specimens

Interspecies versus Species-Specific.

1960 ◽  
Vol 15 (10) ◽  
pp. 665-665
Author(s):  
George S. Grosser
Keyword(s):  
Species Specific

Species Specific Immunoassays to Measure Blood Platelet and Coagulation Activation in the Rat

1996 ◽  
Vol 76 (06) ◽  
pp. 1090-1095 ◽  
Author(s):  
C Ravanat ◽  
M Freund ◽  
S Schuhler ◽  
P Grunert ◽  
L Meyer ◽  
...  
Keyword(s):  
Antithrombin Iii ◽  
Plasma Concentrations ◽  
Simultaneous Detection ◽  
Blood Platelet ◽  
Coagulation Activation ◽  
Platelet Factor ◽  
Prethrombotic State ◽  
Low Levels ◽  
Species Specific ◽  
Higher Sensitivity

SummaryThe purpose of this study was to develop specific and sensitive immunoassays to detect early indices of hypercoagulability in the rat. Rat platelet factor 4 (rPF4) and rat fibrinopeptide A (rFPA) assays, tools for the detection of activation of platelets and coagulation respectively, were designed using antibodies raised against purified rPF4 and against synthetic rFPA. The relevance of these new assays and of the commercially available ELISA kit for thrombin-antithrombin III (TAT) complexes was demonstrated in a rat model of a prethrombotic state induced by intravenous infusion of varying doses of thromboplastin (90 to 2400 μl/kg/h). In this model, the immunoassays allowed simultaneous detection of low levels of rFPA and rPF4 which were correlated with fibrinogen and platelet consumption and TAT generation and further proved to be of higher sensitivity than the classical methods of platelet count or measurement of fibrinogen levels. Plasma concentrations of rFPA, rPF4 and TAT were dependent on infusion time and thromboplastin dose, while hirudin (1 mg/kg) prevented their appearance. Thus the new specific immunoassays for rPF4 and rFPA and the commercial human TAT assay represent useful tools for pathophysiological studies or the screening of antithrombotic drugs in rats.

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