Identification of differentially expressed genes in brown planthopperNilaparvata lugens(Hemiptera: Delphacidae) responding to host plant resistance
AbstractThe brown planthopperNilaparvata lugensStål is one of the major insect pests of riceOryza sativaL. The host resistance exhibits profound effects on growth, development and propagation ofN. lugens. To investigate the molecular response ofN. lugensto host resistance, a cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique was employed to identify the differentially expressed genes in the nymphs feeding on three rice varieties. Of the 2800 cDNA bands analysed, 54 were up-regulated and seven down-regulated qualitatively inN. lugenswhen the ingestion sources were changed from susceptible rice plants to resistant ones. Sequence analysis of the differential transcript-derived fragments showed that the genes involved in signalling, stress response, gene expression regulation, detoxification and metabolism were regulated by host resistance. Four of the transcript-derived fragments corresponding to genes encoding for a putative B subunit of phosphatase PP2A, a nemo kinase, a cytochrome P450 monooxygenase and a prolyl endopeptidase were further characterized in detail. Northern blot analysis confirmed that the expression of the four genes was enhanced inN. lugensfeeding on resistant rice plants. The roles of these genes in the defensive response ofN. lugensto host plant resistance were discussed.