Enhancement of embryogenesis and plant regeneration from barley anther culture by low doses of gamma irradiation

The experiment was conducted to investigate the performance of three different genotypes (BARI Sarisha-6, BARI Sarisha-8, and BARI Sarisha-11) in two different media viz., MS and B5 with different concentrations of phytohormone (2, 4-D) for callus induction from uninucleate stage anthers of Brassica and subsequent plant regeneration in MS media with different concentrations of phytohormone (BAP and NAA). Among the genotypes, BARI Sarisha-8 showed the best performance for all the parameters of callus induction. The performance of BARI Sarisha-6 was poor compared to others. Maximum rate of callus induction (%) was observed in MS + 0.5 mg/L 2, 4-D followed by B5 + 0.5 mg/L 2,4-D. The media combination MS + 1.0 mg/L BAP 0.3 mg/L 2,4-D showed the best performance for maintenance of calli. Significant variations were observed among the genotypes and media composition for shoot regeneration. Among the genotypes, BARI Sarisha-8 showed the best performance for shoot regeneration followed by BARJ Sarisha-l1. The genotype BARI Sarisha-8 produced higher percent of shoots/calli and required minimum days for shoot initiation. Higher percent calli without shoot were produced by the genotype BARI Sarisha-6. The media combination MS + 2.0 mg/L BAP + 0.5 mg/L NAA showed the best performance for shoot regeneration and required maximum days for shoot initiation. Keywords: Regeneration; BARI Sarisha-6; BARI Sarisha-8; BARI Sarisha-11; anther culture; phytohormone DOI: 10.3329/bjar.v35i2.5896Bangladesh J. Agril. Res. 35(2) : 331-341, June 2010

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Impact of Low Doses of Gamma Irradiation on Off-Season Guava at Ambient Storage Condition

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2018.701.032 ◽

2018 ◽

Vol 7 (1) ◽

pp. 295-307 ◽

Cited By ~ 2

Author(s):

Sayan Sau ◽

Pallab Datta ◽

Tanmoy Sarkar ◽

Sukamal Sarkar

Keyword(s):

Gamma Irradiation ◽

Storage Condition ◽

Low Doses ◽

Ambient Storage

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Regenerasi dan Aklimatisasi Kultur Antera Enam Persilangan F1 Padi Sawah

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) ◽

10.24831/jai.v44i2.13479 ◽

2016 ◽

Vol 44 (2) ◽

pp. 133

Author(s):

Cucu Gunarsih ◽

Bambang Sapta Purwoko ◽

Iswari Saraswati Dewi ◽

Dan Muhamad Syukur

Keyword(s):

Plant Regeneration ◽

Anther Culture ◽

Doubled Haploid ◽

Callus Induction ◽

Rainfed Rice ◽

Randomized Design ◽

Doubled Haploid Plants ◽

Completely Randomized Design ◽

N6 Medium ◽

Haploid Plants

ABSTRACT The breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing: INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM putresin, and media for rooting was based on MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%, respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants), G5 (21 plants), and G6 (26 plants). Keywords: Callus induction, doubled haploid, rice

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