The combined use of triacylglycerols containing medium-chain fatty acids and exogenous lipolytic enzymes as an alternative to in-feed antibiotics in piglets: concept, possibilities and limitations. An overview

In the search for alternatives to banned in-feed antibiotics, a concept was developed based on studies with medium-chain fatty acid-containing triacylglycerols (MCTAG) and selected lipases forin situgeneration of diacylglycerols, monoacylglycerols and medium-chain fatty acids (MCFA) in the stomach and proximal gut of piglets. MCFA are known to have strong antibacterial properties but can hardly be used as such because of their repellent odour and taste. Those problems could be overcome by the generation of MCFAin situ. The concept was testedin vitroand validatedin vivowith gastric-cannulated piglets and under field conditions, including effects on zootechnical performance, with classical antibacterial growth promoters or organic acids acting as positive controls. Furthermore, the metabolic and dietary constraints on the nutritional and nutritive use of MCTAG and/or MCFA (for example, the effects on digestive physiology, gut flora, feed intake, performance, carcass composition) are reviewed. The role of natural preduodenal lipase activity, the presence of endogenous plant lipase activity in raw materials and the feasibility for exogenous lipase addition to the feed are discussed, in order to optimize the concept. The present review illustrates the similarity of the action of MCFA and commonly used antimicrobials on the flora (total flora, Gram-positive flora, Gram-negative flora, potential pathogens) and epithelial morphology and histology in the foregut. These observations are believed to be the basis for obtaining optimal growth performances. In addition, these naturally occurring antimicrobial agents have little or no human or animal toxicity and induce no problems of residues and cross-resistance induction. They are proposed as a valuable alternative to in-feed antibiotics, used for growth promotion, and even for the preventive and curative treatment of gastrointestinal diseases.

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Ionotropic receptors in the turnip moth Agrotis segetum respond to repellent medium-chain fatty acids

10.1101/2021.02.25.432965 ◽

2021 ◽

Author(s):

Xiao-Qing Hou ◽

Dan-Dan Zhang ◽

Daniel Powell ◽

Hong-Lei Wang ◽

Martin N. Andersson ◽

...

Keyword(s):

Fatty Acids ◽

Octanoic Acid ◽

Odorant Receptors ◽

Agrotis Segetum ◽

Nonanoic Acid ◽

Ionotropic Receptors ◽

Medium Chain ◽

Medium Chain Fatty Acids ◽

Chain Fatty Acids

In insects, airborne chemical signals are mainly detected by two receptor families, odorant receptors (ORs) and ionotropic receptors (IRs). Functions of ORs have been intensively investigated in Diptera and Lepidoptera, while the functions and evolution of the more ancient IR family remain largely unexplored beyond Diptera. Here, we identified a repertoire of 26 IRs from transcriptomes of female and male antennae, and ovipositors in the moth Agrotis segetum. We observed that a large clade formed by IR75p and IR75q expansions is closely related to the acid-sensing IRs identified in Diptera. We functionally assayed each of the five AsegIRs from this clade using Xenopus oocytes and found that two receptors responded to the tested ligands. AsegIR75p.1 responded to several compounds but hexanoic acid was revealed to be the primary ligand, and AsegIR75q.2 responded primarily to octanoic acid, and less so to nonanoic acid. It has been reported that the C6-C10 medium-chain fatty acids repel various insects including many drosophilids and mosquitos. Our GC-EAD recordings showed that C6-C10 medium-chain fatty acids elicited antennal responses of both sexes of A. segetum, while only octanoic acid had repellent effect to the moths in a behavioural assay. In addition, using fluorescence in situ hybridization, we demonstrated that AsegIR75q.2 and its co-receptor AsegIR8a are not located in coeloconic sensilla as found in Drosophila, but in basiconic or trichoid sensilla. These functional data in combination with our phylogenetic analysis suggest that subfunctionalization of the acid-sensing IRs after gene duplication plays an important role in the evolution of ligand specificities of the acid-sensing IRs in Lepidoptera.

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Effect Of Dietary Medium-Chain Fatty Acids On Campylobacter Jejuni In Broiler Chickens

Scientia Agriculturae Bohemica ◽

10.1515/sab-2015-0030 ◽

2015 ◽

Vol 46 (4) ◽

pp. 154-158

Author(s):

P. Hovorková ◽

E. Skřivanová ◽

E. Kudrnová ◽

M. Marounek

Keyword(s):

Fatty Acids ◽

Campylobacter Jejuni ◽

Broiler Chickens ◽

Antibacterial Properties ◽

Medium Chain ◽

Medium Chain Fatty Acids ◽

Beneficial Impact ◽

Chain Fatty Acids

Abstract The inhibitory properties of a commercial product Fortibac® containing medium-chain fatty acids on Campylobacter jejuni were determined. The product is a mixture of C6:0-C14:0 fatty acids. After testing the antibacterial properties towards C. jejuni in in vitro conditions, an experimental infection on broiler chickens was performed to confirm the results. The product was admixed with feed (final concentrations 0, 0.25, and 0.5%) and broiler chickens were artificially infected with C. jejuni VFU 612. The chickens were infected on day 16 of age, while the aforementioned feed mixtures were used during the entire fattening period (days 0–35). After the infection, the dynamics of C. jejuni shedding was evaluated among treated groups and the control. Reduction of the number of campylobacters by the product with medium-chain fatty acids was not confirmed in vivo. It is assumed that the final amount of potentially active fatty acids in the digestive tract was not sufficient. The product, however, had a clear beneficial impact on mortality of infected chickens.

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Enhanced Bioavailability of Curcumin Nanoemulsions Stabilized with Phosphatidylcholine Modified with Medium Chain Fatty Acids

Current Drug Delivery ◽

10.2174/1567201813666160919142811 ◽

2017 ◽

Vol 14 (3) ◽

pp. 377-385 ◽

Cited By ~ 8

Author(s):

Angelica A. Ochoa-Flores ◽

Josafat A. Hernández-Becerra ◽

Adriana Cavazos-Garduño ◽

Ida Soto-Rodríguez ◽

Maria Guadalupe Sanchez-Otero ◽

...

Keyword(s):

Fatty Acids ◽

Medium Chain ◽

Medium Chain Fatty Acids ◽

Chain Fatty Acids

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Effects of ketogenic diet containing medium-chain fatty acids on serum inflammatory factor and mTOR signaling pathway in rats

Chemical and Biological Technologies in Agriculture ◽

10.1186/s40538-020-00194-4 ◽

2020 ◽

Vol 7 (1) ◽

Author(s):

Huan Liu ◽

Jingwei Huang ◽

Hui Liu ◽

Feng Li ◽

Quansheng Peng ◽

...

Keyword(s):

Fatty Acids ◽

Caloric Restriction ◽

Ketogenic Diet ◽

Mtor Pathway ◽

Inflammatory Factor ◽

Medium Chain ◽

Medium Chain Fatty Acids ◽

Sd Rats ◽

Anti Inflammatory ◽

Chain Fatty Acids

Abstract Background The ketogenic diet (KD) can promote the anti-inflammatory metabolic state and increase ketone body level in rats. This study was to explore the effects and differences of KD with or without medium-chain fatty acids (MCFAs) on serum inflammatory factors and mTOR pathway in Sprague–Dawley (SD) rats. Results Male SD rats were assigned to five groups: control diet (C), 20% caloric restriction diet (LC), 20% caloric restriction ketogenic diet (containing MCFAs) (LCKD1), 20% caloric restriction ketogenic diet (LCKD2) and 20% caloric restriction foreign ketogenic diet (LCKD3), and fed for 30 d. LC and KD could significantly reduce the body weight of rats; LC and KD containing MCFAs showed anti-inflammatory effects; KD without MCFAs decreased the concentration of mTOR1, while KD containing MCFAs decreased the expression of AMPK, mtor1 and P70sk. Conclusions KD containing MCFAs showed better effects on the mTOR pathway and anti-inflammation than that without MCFAs.

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