APOPTOSIS ONSET AND BAX PROTEIN DISTRIBUTION IN SPINAL MOTONEURONS OF NEWBORN RATS FOLLOWING SCIATIC NERVE AXOTOMY

2003 ◽  
Vol 113 (9) ◽  
pp. 1163-1175 ◽  
Author(s):  
TAKI TIRAIHI ◽  
MOHAMMED JAFFER REZAIE
Keyword(s):  
Sciatic Nerve ◽  
Protein Distribution ◽  
Newborn Rats ◽  
Spinal Motoneurons ◽  
Bax Protein

Somatostatin Type 2 Receptor Antibody Enhances Mechanical Hyperalgesia in the Dorsal Root Ganglion Neurons after Sciatic Nerve-pinch Injury: Evidence of Behavioral Studies and Bax Protein Expression

2020 ◽  
Vol 18 (10) ◽  
pp. 791-797
Author(s):  
Qiong Xiang ◽  
Jing-Jing Li ◽  
Chun-Yan Li ◽  
Rong-Bo Tian ◽  
Xian-Hui Li
Keyword(s):  
Sciatic Nerve ◽  
Dorsal Root Ganglion ◽  
Nerve Injury ◽  
Dorsal Root ◽  
Underlying Mechanism ◽  
Dorsal Root Ganglion Neurons ◽  
Mechanical Hyperalgesia ◽  
Bax Protein ◽  
Ganglion Neurons

Background: Our previous study has indicated that somatostatin potently inhibits neuropathic pain through the activation of its type 2 receptor (SSTR2) in mouse dorsal root ganglion and spinal cord. However, the underlying mechanism of this activation has not been elucidated clearly Objective: The aim of this study is to perform the pharmacological studies on the basis of sciatic nerve-pinch mice model and explore the underlying mechanism involving SSTR2. Methods: On the basis of a sciatic nerve-pinch injury model, we aimed at comparing the painful behavior and dorsal root ganglion neurons neurochemical changes after the SSTR2 antibody (anti- SSTR2;5μl,1μg/ml) administration in the mouse. Results: After pinch nerve injury, we found that the mechanical hyperalgesia and severely painful behavior (autotomy) were detected after the application of SSTR2 antibody (anti-SSTR2; 5μl, 1μg/ml) on the pinch-injured nerve. The up-regulated phosphorylated ERK (p-ERK) expression and the apoptotic marker (i.e., Bax) were significantly decreased in DRGs after anti-SSTR2 treatment. Conclusion: The current data suggested that inhibitory changes in proteins from the apoptotic pathway in anti-SSTR2-treated groups might be taking place to overcome the protein deficits caused by SSTR2 antibody and supported the new therapeutic intervention with SSTR2 antagonist for neuronal degeneration following nerve injury.

Netrin G-2 ligand mRNA is downregulated in spinal motoneurons after sciatic nerve lesion

Neuroreport ◽  
2010 ◽  
Vol 21 (11) ◽  
pp. 782-785 ◽  
Author(s):  
Alexander Berg ◽  
Johan Zelano ◽  
Staffan Cullheim
Keyword(s):  
Sciatic Nerve ◽  
Nerve Lesion ◽  
Spinal Motoneurons ◽  
Sciatic Nerve Lesion

Glutamate receptors in spinal motoneurons after sciatic nerve transection

Neuroscience ◽  
1996 ◽  
Vol 74 (4) ◽  
pp. 953-958 ◽  
Author(s):  
A. Popratiloff ◽  
V.N. Kharazia ◽  
R.J. Weinberg ◽  
B. Laonipon ◽  
A. Rustioni
Keyword(s):  
Sciatic Nerve ◽  
Glutamate Receptors ◽  
Nerve Transection ◽  
Spinal Motoneurons ◽  
Sciatic Nerve Transection

scholarly journals Effects of Deep Electroacupuncture Stimulation at “Huantiao” (GB 30) on Expression of Apoptosis-Related Factors in Rats with Acute Sciatic Nerve Injury

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Lili Dai ◽  
Yanjing Han ◽  
Tieming Ma ◽  
Yuli Liu ◽  
Lu Ren ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Normal Control ◽  
Expression Level ◽  
Control Group ◽  
Model Group ◽  
Related Factors ◽  
Treatment Groups ◽  
Bax Protein ◽  
Hip Muscle ◽  
Nerve Muscle

SD rats were randomly divided into normal control, model, deep EA, and shallow EA groups. The model was established by mechanical clamping of the sciatic nerve stem. For deep and shallow EA, the needles were inserted into “Huantiao” (GB 30) by about 16 mm and 7 mm, respectively, once daily for 14 days. The results showed that, compared with the normal control group, the nerve-muscle excitability of rat’s hip muscle decreased and the nerve conduction velocity of sciatic nerve slowed down in the model group; meanwhile, the number of apoptotic cells and the expression level of Bax protein in the injured nerve increased significantly, and the expression level of Bcl-2 protein and the ratio of Bcl-2/Bax decreased considerably. Compared with the model group, the indices mentioned above were reversed in the two treatment groups, and the changes in the deep EA group were more significant than those in the shallow EA group. These results indicate that EA stimulation at GB 30 can improve the function of injured sciatic nerve, which is closely associated with its effects in upregulating the expression of apoptosis inhibitive factor Bcl-2 and downregulating apoptosis promotive factor Bax. Deep EA is relatively better.

Inhibition of caspases promotes long-term survival and reinnervation by axotomized spinal motoneurons of denervated muscle in newborn rats

2003 ◽  
Vol 181 (2) ◽  
pp. 190-203 ◽  
Author(s):  
Yuen-Man Chan ◽  
Leung-Wah Yick ◽  
Henry K Yip ◽  
Kwok-Fai So ◽  
Ronald W Oppenheim ◽  
...  
Keyword(s):  
Newborn Rats ◽  
Spinal Motoneurons ◽  
Denervated Muscle ◽  
Term Survival ◽  
Long Term Survival
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