Directed evolution of yeast ferric reductase to produce plants with tolerance to iron deficiency in alkaline soils

2004 ◽  
Vol 50 (7) ◽  
pp. 1159-1165 ◽  
Author(s):  
Hiroyuki Oki ◽  
Suyeon Kim ◽  
Hiromi Nakanishi ◽  
Michiko Takahashi ◽  
Hirotaka Yamaguchi ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Directed Evolution ◽  
Alkaline Soils ◽  
Ferric Reductase

scholarly journals Understanding the Role of the Antioxidant System and the Tetrapyrrole Cycle in Iron Deficiency Chlorosis

Plants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 348 ◽  
Author(s):  
Carla S. Santos ◽  
Rengin Ozgur ◽  
Baris Uzilday ◽  
Ismail Turkan ◽  
Mariana Roriz ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Iron Deficiency ◽  
Antioxidant System ◽  
Reductase Activity ◽  
Activity Levels ◽  
Alkaline Soils ◽  
Ferric Reductase ◽  
Ros Scavenging ◽  
Iron Deficiency Chlorosis ◽  
No Formation

Iron deficiency chlorosis (IDC) is an abiotic stress often experienced by soybean, owing to the low solubility of iron in alkaline soils. Here, soybean lines with contrasting Fe efficiencies were analyzed to test the hypothesis that the Fe efficiency trait is linked to antioxidative stress signaling via proper management of tissue Fe accumulation and transport, which in turn influences the regulation of heme and non heme containing enzymes involved in Fe uptake and ROS scavenging. Inefficient plants displayed higher oxidative stress and lower ferric reductase activity, whereas root and leaf catalase activity were nine-fold and three-fold higher, respectively. Efficient plants do not activate their antioxidant system because there is no formation of ROS under iron deficiency; while inefficient plants are not able to deal with ROS produced under iron deficiency because ascorbate peroxidase and superoxide dismutase are not activated because of the lack of iron as a cofactor, and of heme as a constituent of those enzymes. Superoxide dismutase and peroxidase isoenzymatic regulation may play a determinant role: 10 superoxide dismutase isoenzymes were observed in both cultivars, but iron superoxide dismutase activity was only detected in efficient plants; 15 peroxidase isoenzymes were observed in the roots and trifoliate leaves of efficient and inefficient cultivars and peroxidase activity levels were only increased in roots of efficient plants.

The growth of Lupinus species on alkaline soils

1995 ◽  
Vol 46 (1) ◽  
pp. 255 ◽  
Author(s):  
C Tang ◽  
AD Robson ◽  
NE Longnecker ◽  
BJ Buirchell
Keyword(s):  
Iron Deficiency ◽  
Seed Yield ◽  
Shoot Growth ◽  
Clay Content ◽  
Field Studies ◽  
Iron Chlorosis ◽  
Alkaline Soils ◽  
Sand Soil ◽  
Poor Growth ◽  
High Concentrations

Lupinus angustifolius L. grows poorly on alkaline soils, particularly those that are fine-textured. This poor growth has been attributed to high concentrations of bicarbonate, high clay content and/or iron deficiency. In field studies, we examined the growth of 13 lupin genotypes reliant on N2 fixation, or receiving NH4N03, at four sites with various combinations of soil pH and texture. Plants grown on an alkaline clay and an alkaline sand showed iron chlorosis at early stages, and had a slower shoot growth than those grown on an acid loam or an acid sand. Species varied greatly in the severity of iron chlorosis and also in growth and seed yield, with L. angustifolius, L. luteus and L. albus more affected than L. pilosus, L. atlanticus and L. cosentinii. Rankings of growth and seed yield of the lupin genotypes on the alkaline clay correlated well with the rankings on the alkaline sand soil. Plants which had severe iron chlorosis in alkaline clay also had severe chlorosis in alkaline sands. However, correlation between the severity of iron chlorosis and early shoot growth was poor. The results suggest that high pH and/or high bicarbonate are more likely than soil texture to be the primary factors restricting the growth of commercial lupins.

scholarly journals Iron Deficiency Induced Changes in Chlorophyll and Ferric Reductase Activity Asian Pear Rootstocks (Pyrus spp.) with Hydroponics

2005 ◽  
Vol 43 (3) ◽  
pp. 173-180
Author(s):  
Chunhui MA ◽  
Kenji TANABE ◽  
Akihiro ITAI ◽  
Yuanwen TENG ◽  
Jong-Pil CHUN
Keyword(s):  
Iron Deficiency ◽  
Reductase Activity ◽  
Ferric Reductase ◽  
Asian Pear ◽  
Induced Changes

Duodenal ascorbate and ferric reductase in human iron deficiency

2005 ◽  
Vol 81 (1) ◽  
pp. 130-133 ◽  
Author(s):  
Bisera D Atanasova ◽  
Andy CY Li ◽  
Ingvar Bjarnason ◽  
Kamen N Tzatchev ◽  
Robert J Simpson
Keyword(s):  
Iron Deficiency ◽  
Ferric Reductase

scholarly journals Plant-derived coumarins shape the composition of an Arabidopsis synthetic root microbiome

2018 ◽  
Author(s):  
Mathias J.E.E.E. Voges ◽  
Yang Bai ◽  
Paul Schulze-Lefert ◽  
Elizabeth S. Sattely
Keyword(s):  
Microbial Community ◽  
Iron Deficiency ◽  
Molecular Mechanisms ◽  
Microbial Community Composition ◽  
Alkaline Soils ◽  
Arable Soils ◽  
Specialized Metabolites ◽  
Soil Inoculum ◽  
The Impact ◽  
Root Microbiome

SignificanceThe root microbiome composition is largely determined by the soil inoculum, with a distinct contribution from the host. Yet, the molecular mechanisms with which the host influences its rhizobiome are only beginning to be discovered. Using a hydroponics-based synthetic community approach, we probe the impact of root-exuded specialized metabolites in shaping the root microbiome. We uncover a role for coumarins in structuring the rhizobiome, particularly by limiting the growth of a Pseudomonas strain, for which we propose a mechanism of action. Our findings support the exciting possibility that root-exuded coumarins form a part of the plant’s adaptive response to iron deficiency that goes beyond iron mobilization to modulate the rhizobiome, and highlights avenues towards engineering the rhizosphere for plant health.AbstractThe factors that dictate the composition of the root microbiome and its role in plant fitness has been a long-standing question. Recent work has highlighted a major contribution of the soil inoculum in determining the composition of the root microbiome. However, plants are known to conditionally exude a diverse array of unique secondary metabolites, largely varying between species and environmental conditions. Here, we explore the role of specialized metabolites in dictating which bacteria reside in the rhizosphere. We employed a reduced synthetic community (SynCom) of Arabidopsis thaliana root-isolated bacteria to detect community shifts that occur in the absence of the secreted small molecule phytoalexins, flavonoids, and coumarins. We find that lack of coumarin biosynthesis in f6’h1 mutant plant lines causes a shift in the root microbial community specifically under iron deficiency. We demonstrate a potential role for iron-mobilizing coumarins in sculpting the A. thaliana root bacterial community by inhibiting the proliferation of a relatively abundant Pseudomonas species via a redox-mediated mechanism. This work establishes a systematic approach enabling elucidation of specific mechanisms by which plant-derived molecules mediate microbial community composition. Our findings expand on the function of conditionally-exuded specialized metabolites and lead to new avenues to effectively engineer the rhizosphere for improving crop growth in alkaline soils, which make up a third of total arable soils.

scholarly journals Identification of iron deficiency chlorosis tolerant sources from mini-core collection of groundnut (Arachis hypogaea L.)

2018 ◽  
Vol 16 (5) ◽  
pp. 446-458 ◽  
Author(s):  
Santosh K. Pattanashetti ◽  
Gopalakrishna K. Naidu ◽  
Prakyath Kumar K.V. ◽  
Omprakash Kumar Singh ◽  
Basavaraj D. Biradar
Keyword(s):  
Iron Deficiency ◽  
Principal Components ◽  
Core Collection ◽  
Growth Stages ◽  
Alkaline Soils ◽  
Iron Deficiency Chlorosis ◽  
Pod Yield ◽  
Mini Core Collection ◽  
Iron Deficient ◽  
Botanical Varieties

AbstractIron deficiency chlorosis (IDC) is common among groundnut grown in calcareous and alkaline soils in India, China and Pakistan and causes considerable reduction in pod yield. To identify genetically diverse IDC tolerant accessions, the mini-core collection of groundnut representing geographical diversity was evaluated for IDC response over 2 years in iron-deficient calcareous soils. Enormous genetic variability was evident in the mini-core collection for IDC tolerance-related traits such as a visual chlorotic rating (VCR) and SPAD chlorophyll meter reading (SCMR) across five growth stages. Several IDC tolerant sources belonging to different botanical varieties such as hypogaea bunch (ICG # 5051, 6766, 5286, 6667, 4538, 14008, 5663, 9842, 11855), hypogaea runner (ICG 10479), fastigiata (ICG 10890) and vulgaris (ICG # 11651, 118) were identified. Among the six botanical varieties of groundnut, hypogaea bunch types were found most tolerant to IDC and this is the first report in groundnut. The IDC tolerant sources identified were irrespective of their country of origin. The principal component analysis based on VCR, SCMR, pod yield and its related traits revealed five major principal components that explained 80% of the total variation. The biplot generated using PC1 and PC2 revealed a distinct separation of IDC tolerant genotypes from the susceptible ones. The hierarchical clustering using five major principal components revealed seven major clusters that were mainly based on IDC response of the accessions.

PAP/SAL1 retrograde signaling pathway modulates iron deficiency response in alkaline soils

Plant Science ◽  
2020 ◽  
pp. 110808
Author(s):  
Manuel Balparda ◽  
Alejandro M. Armas ◽  
Diego F. Gomez-Casati ◽  
María Ayelén Pagani
Keyword(s):  
Iron Deficiency ◽  
Signaling Pathway ◽  
Retrograde Signaling ◽  
Alkaline Soils
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