Physico-Chemical Properties of the Most Suitable Spray Reagent for Fluorescence Enhancement in Thin-Layer Chromatography

1980 ◽  
Vol 3 (5) ◽  
pp. 681-691 ◽  
Author(s):  
S. Uchiyama ◽  
M. Uchiyama
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Fluorescence Enhancement ◽  
Chemical Properties ◽  
Spray Reagent ◽  
Physico Chemical ◽  
Layer Chromatography

scholarly journals ISOLATION AND ANALYSIS OF STEROIDAL SAPONINS FROM POLYGONATUM ODORATUM (MILL.) DRUCE

2021 ◽  
Vol 55 ◽  
pp. 135-140
Author(s):  
Cristina MOGOSAN ◽  
Ilioara ONIGA ◽  
Mircea TAMAS
Keyword(s):  
Thin Layer ◽  
Acid Hydrolysis ◽  
Thin Layer Chromatography ◽  
Biological Properties ◽  
Steroidal Saponins ◽  
Physico Chemical ◽  
Layer Chromatography ◽  
Hydrolysis Of

We isolated the steroidal saponins from the rhizomes of Polygonatum odoratum (Mill.) Druce with an efficiency of 4.50% which represents 7 fractions identified by thin-layer chromatography (TLC), of which 3 were furostanics and 4 spirostanics. After the acid hydrolysis of the saponins, one aglycone (sapogenine) was identified by TLC. Further, we have determined the physico-chemical and the biological properties of the isolated saponins.

CAFFEINE ANALYSIS BY THIN-LAYER CHROMATOGRAPHY RELATED TO THE PHYSICAL-CHEMICAL PROPERTIES OF DIFFERENT VARIETIES OF COFFEE AND SURROGATES

2017 ◽  
Vol 31 (1) ◽  
pp. 69-73
Author(s):  
Cristina Mihali ◽  
◽  
Ionut Grad ◽  
Thomas Dippong ◽  
◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chemical Properties ◽  
Chemical Characteristics ◽  
Physical Chemical ◽  
Different Types ◽  
Decaffeinated Coffee ◽  
Layer Chromatography

The aim of this study was to evaluate the physical-chemical characteristics of different types of coffee, decaffeinated and of two types of coffee substitutes based on barley and chicory. The following physical-chemical characteristics were performed: moisture, pH, content of polyphenols and qualitatively determination of caffeine, by thin-layer chromatography. The moisture of the investigated coffee and substitutes of coffee was in the range 1,5 and 3,6%. By analyzing the moisture of the coffee substitutes, we observed that coffee based on barley has the highest moisture and chicory has the lowest value. The caffeine was qualitatively determined by thin-layer chromatography. It was found that all types of the investigated samples contain caffeine excepting decaffeinated coffee. Polyphenols content of the analysed coffee samples varied in the range of 2.8-3.2 mg/g.

Spray reagent for the detection of amino acids on thin layer chromatography plates

Amino Acids ◽  
1993 ◽  
Vol 4 (1-2) ◽  
pp. 193-196 ◽  
Author(s):  
B. Basak ◽  
U. K. Bhattacharyya ◽  
S. Laskar
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Spray Reagent ◽  
Layer Chromatography

Use of Fluorescamine ("Fluram") to Detect Amphetamine in Urine by Thin-Layer Chromatography

1974 ◽  
Vol 20 (2) ◽  
pp. 272-274 ◽  
Author(s):  
Bernard Klein ◽  
Joan E Sheehan ◽  
E Grunberg
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Drugs Of Abuse ◽  
Thin Layer Plate ◽  
Spray Reagent ◽  
Layer Chromatography

Abstract A "Fluram" (fluorescamine; 4-phenylspiro[furan-2(3H)-1'-phthalan]-3,3'-dione) spray reagent will detect as little as 250 ng of amphetamine in an extract of amphetamine-containing urine, after it has been separated by thin-layer chromatography. The fluorescence is stable for about 20 h and can be renewed by respraying. Fluram spray does not interfere with reagent sprays used to detect other drugs of abuse. Amphetamine on the thin-layer plate can be measured by extracting the fluorescent area of silica gel and measuring the fluorescence in a fluorimeter. Recovery of 1.0 µg and 2.0 µg of amphetamine averaged 69% and 67%, respectively.

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