Preparative Separation of Glabridin fromGlycyrrhiza glabraL. Extracts with Macroporous Resins

2009 ◽  
Vol 44 (15) ◽  
pp. 3717-3734 ◽  
Author(s):  
Yan Xu ◽  
Qipeng Yuan ◽  
Xiaodan Hou ◽  
Yanping Lin
Keyword(s):  
Preparative Separation ◽  
Macroporous Resins

scholarly journals Separation and Purification of Two Flavone Glucuronides fromErigeron multiradiatus(Lindl.) Benth with Macroporous Resins

2009 ◽  
Vol 2009 ◽  
pp. 1-8 ◽  
Author(s):  
Zhi-feng Zhang ◽  
Yuan Liu ◽  
Pei Luo ◽  
Hao Zhang
Keyword(s):  
Large Scale ◽  
Raw Materials ◽  
Sorption Isotherms ◽  
Preparative Separation ◽  
Separation And Purification ◽  
Bioactive Constituents ◽  
Simple Method ◽  
Scale Separation ◽  
Adsorption And Desorption ◽  
Macroporous Resins

Scutellarein-7-O-β-D-glucuronide (SG) and apigenin-7-O-β-D-glucuronide (AG) are two major bioactive constituents with known pharmacological effects inErigeron multiradiatus. In this study, a simple method for preparative separation of the two flavone glucuronides was established with macroporous resins. The performance and adsorption characteristics of eight macroporous resins including AB-8, HPD100, HPD450, HPD600, D100, D101, D141, and D160 have been evaluated. The results confirmed that D141 resin offered the best adsorption and desorption capacities and the highest desorption ratio for the two glucuronides among the tested resins. Sorption isotherms were constructed for D141 resin under optimal ethanol conditions and fitted well to the Freundlich and Langmuir models (R2>0.95). Dynamic adsorption and desorption tests was performed on column packed with D141 resin. After one-run treatment with D141 resin, the two-constituent content in the final product was increased from 2.14% and 1.34 % in the crude extract ofErigeron multiradiatusto 24.63% and 18.42% in the final products with the recoveries of 82.5% and 85.4%, respectively. The preparative separation of SG and AG can be easily and effectively achieved via adsorption and desorption on D141 resin, and the method developed can be referenced for large-scale separation and purification of flavone glucuronides from herbal raw materials.

Preparative separation of vitexin and isovitexin from pigeonpea extracts with macroporous resins

2007 ◽  
Vol 1139 (2) ◽  
pp. 206-213 ◽  
Author(s):  
Yujie Fu ◽  
Yuangang Zu ◽  
Wei Liu ◽  
Chunlian Hou ◽  
Liyan Chen ◽  
...  
Keyword(s):  
Preparative Separation ◽  
Macroporous Resins

scholarly journals Preparative Separation and Enrichment of Syringopicroside fromFolium syringaeLeaves with Macroporous Resins

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Xin Liu ◽  
Jianming Wang ◽  
Changxin Zhou ◽  
Lishe Gan
Keyword(s):  
Large Scale ◽  
Packed Column ◽  
Major Constituent ◽  
Preparative Separation ◽  
Simple Method ◽  
Adsorption And Desorption ◽  
Macroporous Resins ◽  
Large Scale Preparation ◽  
Different Temperatures ◽  
Scale Preparation

Syringopicroside is the major constituent inFolium syringaeleaves with known pharmacological activities. In this study, a simple method for preparative separation of syringopicroside fromF. syringaeleaves with macroporous resins was developed. Adsorption characteristics of syringopicroside on six types of macroporous resins, including ADS-8, ADS-17, D141, NKA-9, HPD450, and HPD600, have been compared, among which D141 resin showed the best adsorption and desorption capacities for syringopicroside. Adsorption isotherms were used to D141 resin at different temperatures and fitted well to Langmuir and Freundlich equations. Dynamic adsorption and desorption tests were performed on D141 resin-packed column to optimize the separation process of syringopicroside. After one run with D141 resin, the content of syringopicroside was increased 24-fold from 2.32% to 55.74% with a recovery yield of 92.16%. The chromatographic process optimized in this work avoids toxic organic solvent and, thus, is a promising basis for large-scale preparation of syringopicroside.

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