Genetic stability analysis of introducedBetula pendula, Betula kirghisorum, andBetula pubescensfamilies in saline-alkali soil of northeastern China

2014 ◽  
Vol 29 (7) ◽  
pp. 639-649 ◽  
Author(s):  
Xi-yang Zhao ◽  
Xiu-yan Bian ◽  
Zhi-xin Li ◽  
Xiu-wei Wang ◽  
Cheng-jun Yang ◽  
...  
Keyword(s):  
Stability Analysis ◽  
Genetic Stability ◽  
Northeastern China ◽  
Alkali Soil

RAPD based genetic stability analysis among micropropagated, synthetic seed derived and hardened plants of Bacopa monnieri (L.): a threatened Indian medicinal herb

2010 ◽  
Vol 33 (1) ◽  
pp. 163-171 ◽  
Author(s):  
Manikandan Ramesh ◽  
Karuppa Pillai Vijayakumar ◽  
Alagarsamy Karthikeyan ◽  
Shunmugiah Karutha Pandian
Keyword(s):  
Stability Analysis ◽  
Genetic Stability ◽  
Bacopa Monnieri ◽  
Medicinal Herb ◽  
Synthetic Seed

scholarly journals Deteksi Kestabilan Genetik Ramet Kelapa Sawit Hasil Kultur In Vitro Menggunakan SSR

2016 ◽  
Vol 44 (1) ◽  
pp. 83
Author(s):  
Yuni Fitri Cahyaningsih ◽  
Ni Made Armini Wiendi ◽  
Dan Nurita Toruan-Mathius
Keyword(s):  
Tissue Culture ◽  
Stability Analysis ◽  
Oil Palm ◽  
Genetic Stability ◽  
Genetic Similarity ◽  
Qualitative Data ◽  
Elaeis Guineensis ◽  
Group Method ◽  
Pair Group

ABSTRACT<br /><br />Commercial production of oil palm ramet requires the guarantee of high genetic stability. The objectives of this research were to determine 1) genetic diversity of ortet as source of explant, and 2) genetic stability of ramet derived from ortet propagated through tissue culture. Genetic stability analysis was done using ramet from five Tenera (D×P) oil palm ortets.As many as 20 ramets were randomly chosen from each ortet. A total of 100 ramets were used for genetic stability analysis. Genetic similarity analysis was analyzed using NTSyspc version 2.1 software with method Similarity for Qualitative Data and Unweighted Pair Group Method Aritmatic (UPGMA). The results indicated 20 SSR primer pairs were polymorphic and could form 44 alleles. As many as 80% of ramets from IS 3 ortet showed genetic similarity ranged from 97-100% to the ortet. All ramets derived from IS 10, IS 20 and IS 40 ortet had 90-100% of genetic similarity to its respective ortet. Futhermore, 95% of ramets from IS 39 ortet had 97-100% of genetic similarity to the ortet.<br /><br />Keywords: Elaeis guineensis Jacq., genetic similarity, tissue culture<br /><br />

scholarly journals Assessment of Genetic Stability on In Vitro And Ex Vitro Plants of Ficus Carica Var. Black Jack Using Issr and Damd Markers

2021 ◽  
Author(s):  
Ankita Rajendra Parab ◽  
Chew Bee Lynn ◽  
Sreeramanan Subramaniam
Keyword(s):  
Stability Analysis ◽  
Genetic Stability ◽  
Similarity Index ◽  
Issr Markers ◽  
Ficus Carica ◽  
Ex Vitro ◽  
Regenerated Plants ◽  
Inter Simple Sequence Repeats ◽  
Dna Primers

Abstract In vitro propagation has been significant in producing a large number of genetically stable regenerated plants. Regenerated Ficus carica var. Black Jack plantlets were established using woody plant medium (WPM) supplemented with 20 µM 6-Benzylaminopurine (BAP) and 8 µM Indole-3-acetic acid (IAA) under different light treatments such as normal fluorescent white light (60 µmol.m− 2.s− 1), and four different LED spectra, white (400– 700nm), blue (440nm), red (660nm) and blue + red (440nm + 660nm). Genetic stability analysis was performed on the in vitro and ex vitro plants of Ficus carica var. Black Jack. Ten (10) primers of each ISSR and DAMD molecular marker were used to assess the genetic stability of the eight (8) samples of Ficus carica var. Black Jack, acquired over two years. The findings of this study revealed that inter simple sequence repeats (ISSR) and directed amplification of minisatellite DNA (DAMD) markers (DNA primers) are efficient in determining the polymorphism and monomorphism percentage among the in vitro and ex vitro samples of Ficus carica var. Black Jack. ISSR markers showed 97.87% of monomorphism whereas DAMD markers showed 100% monomorphism. Polymorphism of 2.13% was observed for the UBC840 ISSR – DNA primer which was negated under the genetic similarity index analysis for the eight samples. It is recommended that genetic stability analysis should be performed for long-term maintenance of micropropagated plants.

Sign in / Sign up

Export Citation Format

Share Document