RAPD based genetic stability analysis among micropropagated, synthetic seed derived and hardened plants of Bacopa monnieri (L.): a threatened Indian medicinal herb

2010 ◽  
Vol 33 (1) ◽  
pp. 163-171 ◽  
Author(s):  
Manikandan Ramesh ◽  
Karuppa Pillai Vijayakumar ◽  
Alagarsamy Karthikeyan ◽  
Shunmugiah Karutha Pandian
2021 ◽  
Vol 18 (24) ◽  
pp. 1439
Author(s):  
Bunyaporn Nichakool ◽  
Amporn Jamphon ◽  
Yupa Pootang-on ◽  
Weerawan Techakriengkrai ◽  
Taweesak Techakriengkrai

Brahmi (Bacopa monnieri) is a medicinal herb containing bioactive compounds (Bacosides) in the saponin group that enhances memory and prevents dementia. Brahmi is not favored for cooking because it is very bitter. Nowadays, most people consume Brahmi as supplementary food, which makes it more expensive than consuming in food form, not getting fibers and other nutrients. Currently, there are only few studies that work to alleviate in Brahmi. Therefore, this study aimed to find out the saponin quantity in Brahmi after some pretreatments and the effect of these treatments on Bacosides and bitterness. Thus, suitable preparation steps for Brahmi have the highest remaining saponin quantity and are consumable. There were various techniques of preparation, depending on the concentration of salt used in crumpling and 1 time boiling of Brahmi to reduce its bitterness. The salt concentrations used in this study were 0, 10, and 20 % (w/w) compared with the fresh herb. The saponin quantity was analyzed by high-performance liquid chromatography (HPLC). The results showed that the total amount of saponin in boiled Brahmi that crumpled with 0, 10, and 20 % were significantly lower than fresh Brahmi (p < 0.05). Brahmi was crumpled with 10 % salt before boiling had the highest total saponin quantity of 2.69±0.02 g/kg of fresh weight and the highest tasting scores. Thus, the preparation of crumpling with 10 % salt before cooking, was suitable because consumers gained the highest saponin and accepted the taste of food containing Brahmi. HIGHLIGHTS Brahmi have many health benefits not only memory enhancer but also prevention of dementia People used Brahmi as supplementary food because it is very bitter and not favored for cooking Reduce the bitter with non chemical as crumpling with 10 % salt concentration before cooking was highest saponin and accepted the taste of food containing Brahmi


Author(s):  
Yuni Fitri Cahyaningsih ◽  
Ni Made Armini Wiendi ◽  
Dan Nurita Toruan-Mathius

ABSTRACT<br /><br />Commercial production of oil palm ramet requires the guarantee of high genetic stability. The objectives of this research were to determine 1) genetic diversity of ortet as source of explant, and 2) genetic stability of ramet derived from ortet propagated through tissue culture. Genetic stability analysis was done using ramet from five Tenera (D×P) oil palm ortets.As many as 20 ramets were randomly chosen from each ortet. A total of 100 ramets were used for genetic stability analysis. Genetic similarity analysis was analyzed using NTSyspc version 2.1 software with method Similarity for Qualitative Data and Unweighted Pair Group Method Aritmatic (UPGMA). The results indicated 20 SSR primer pairs were polymorphic and could form 44 alleles. As many as 80% of ramets from IS 3 ortet showed genetic similarity ranged from 97-100% to the ortet. All ramets derived from IS 10, IS 20 and IS 40 ortet had 90-100% of genetic similarity to its respective ortet. Futhermore, 95% of ramets from IS 39 ortet had 97-100% of genetic similarity to the ortet.<br /><br />Keywords: Elaeis guineensis Jacq., genetic similarity, tissue culture<br /><br />


2014 ◽  
Vol 29 (7) ◽  
pp. 639-649 ◽  
Author(s):  
Xi-yang Zhao ◽  
Xiu-yan Bian ◽  
Zhi-xin Li ◽  
Xiu-wei Wang ◽  
Cheng-jun Yang ◽  
...  

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