Co-infection of bean yellow mosaic virus and cucumber mosaic virus in Gladiolus sp. plants: phylogenetic analysis of Ukrainian isolates

2021 ◽  
pp. 1-28
Author(s):  
Roksolana S. Sovinska ◽  
Alina A. Dunich ◽  
Lidiya T. Mishchenko
Keyword(s):  
Phylogenetic Analysis ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus

scholarly journals Phylogenetic Analysis of Bean yellow mosaic virus Isolates from Four Continents: Relationship Between the Seven Groups Found and Their Hosts and Origins

Plant Disease ◽  
2008 ◽  
Vol 92 (12) ◽  
pp. 1596-1603 ◽  
Author(s):  
S. J. Wylie ◽  
B. A. Coutts ◽  
M. G. K. Jones ◽  
R. A. C. Jones
Keyword(s):  
Phylogenetic Analysis ◽  
Mosaic Virus ◽  
Bean Yellow Mosaic Virus ◽  
Host Specialization ◽  
Yellow Mosaic Virus ◽  
Nucleotide Substitutions ◽  
Natural Hosts ◽  
Plant Families ◽  
Natural Host Range ◽  
Intragroup Diversity

Genetic diversity of Bean yellow mosaic virus (BYMV) was studied by comparing sequences from the coat protein (CP) and genome-linked viral protein (VPg) genes of isolates from four continents. CP sequences compared were those of 17 new isolates and 47 others already on the database, while the VPg sequences used were from four new isolates and 10 from the database. Phylogenetic analysis of the CP sequences revealed seven distinct groups, six polytypic and one monotypic. The largest and most genetically diverse polytypic group, which had intragroup diversity of 0.061 nucleotide substitutions per site, contained isolates from natural infections in eight host species. These original isolation hosts included both wild (four) and domesticated (four) species and were from monocotyledonous and dicotyledonous plant families, indicating a generalized natural host range strategy. Only one of the other five polytypic groups spanned both monocotyledons and dicotyledons, and all contained isolates from fewer species (one to four), all of which were domesticated and had lower intragroup diversity (0.019 to 0.045 nucleotide substitutions per site), indicating host specialization. Phylogenetic analysis of the fewer VPg sequences revealed three polytypic and two monotypic groupings. These groups also correlated with original natural isolation hosts, but the branch topologies were sometimes incongruous with those formed by CPs. Also, intragroup diversity was generally higher for VPgs than for CPs. A plausible explanation for the groups found when the 64 different CP sequences were compared is that the generalized group represents the original ancestral type from which the specialist host groups evolved in response to domestication of plants after the advent of agriculture. Data on the geographical origins of the isolates within each group did not reveal whether the specialized groups might have coevolved with their principal natural hosts where these were first domesticated, but this seems plausible.

scholarly journals Susceptibility of pea, horse bean and bean to viruses in dependence on the age of the inoculated plants

2013 ◽  
Vol 38 (2) ◽  
pp. 159-172
Author(s):  
Władysław Błaszczak ◽  
Grażyna Ellmann-Wąsik ◽  
Renata Lesiak-Jerzyk
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Broad Bean ◽  
Bean Yellow Mosaic Virus ◽  
Growth And Yield ◽  
Yellow Mosaic Virus ◽  
Susceptibility To Infection ◽  
Age Dependent ◽  
Resistance To Infection

Three cultivars of pea did not differ in their susceptibility to Cucumber Mosaic Virus (CMV) notwithstanding the age of the inoculated plants. But their susceptibility to infection with Bean Yellow Mosaic Virus (BYMV) differed. Horse bean cultivars 'Nadwiślański' and 'Major' proved to be less susceptible to Broad Bean True Mosaic Virus (BBTMV) when older plants were-inoculated. Two bean cultivars 'Złota Saxa' and 'Earle' appeared to be susceptible to BBTMV only in the phase of developing primary leaves and the age-dependent resistance to infection increased faster in plants of the cv. 'Złota Saxa'. Both cultivars of bean showed also age-dependent resistance to infection by BYMV. All these viruses restricted growth and yield of plants. The decreases were greater when younger plants were inoculated. These dependences appeared most distinctly in pea cv. 'Sześciotygodniowy' infected with CMV and in two cultivars of bean infected with BYMV.

scholarly journals Affection of Gladiolus plants with Bean Yellow Mosaic Virus, Cucumber Mosaic Virus and Tobacco Ringspot Virus in some Northern and Central regions of Ukraine

2020 ◽  
Vol 81 (2) ◽  
pp. 36-42
Author(s):  
R. Sovinska ◽  
A. Dunich ◽  
L. Mishchenko
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Viral Infections ◽  
Flower Color ◽  
Bean Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tobacco Ringspot Virus

Gladioli can be affected with 15 species of viruses, which cause significant economic losses to both floriculture and agriculture. The most prevalent and harmful for gladioli are Bean yellow mosaic virus (BYMV) and Cucumber mosaic virus (CMV), circulating on the territory of Ukraine on vegetable, legume and other crops, and also Tobacco ringspot virus (TRSV), which is included into the List of regulated pests of Ukraine and is the subject to strict control. The aim of the work was to conduct testing of gladiolus plants for the presence of viral infection symptoms and to test them for the affection with the most widespread and dangerous viruses, namely: BYMV, CMV, TRSV. Visual diagnostics, enzyme-linked immunosorbent assay in DAS-ELISA modification, transmission electron microscopy method and statistical data analysis were used in this research. The results of the studies showed absence of TRSV in all tested samples. For the first time in Ukraine, it has been established that gladioli are infected by Bean yellow mosaic virus. Its circulation on gladioli has been registered in Poltava, Kyiv and Sumy regions. Gladioli were also found to be affected by CMV or mixed infection of these pathogens. Gladioli infection by BYMV and CMV in Kyiv region is 88.2% and 93.8%, in Poltava – 69.2% and 55.5%, in Sumy – 66.6% and 0%, respectively. Typical symptoms on gladiolus plants caused by CMV and BYMV isolates are leaf chlorotic stripes and flower color break, less often – spotting on the leaves and plant stunting. It has been revealed that affection of gladioli by CMV and BYMV can be asymptomatic. The diversity, nature and course of viral infections in gladioli demonstrate the relevance of further research and their monitoring in Ukraine.

scholarly journals High Prevalence of Three Potyviruses Infecting Cucurbits in Oklahoma and Phylogenetic Analysis of Cucurbit Aphid-Borne Yellows Virus Isolated from Pumpkins

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 53
Author(s):  
Vivek Khanal ◽  
Harrington Wells ◽  
Akhtar Ali
Keyword(s):  
Phylogenetic Analysis ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
High Prevalence ◽  
The Us ◽  
Close Relationship ◽  
The Status ◽  
Low Incidence

Field information about viruses infecting crops is fundamental for understanding the severity of the effects they cause in plants. To determine the status of cucurbit viruses, surveys were conducted for three consecutive years (2016–2018) in different agricultural districts of Oklahoma. A total of 1331 leaf samples from >90 fields were randomly collected from both symptomatic and asymptomatic cucurbit plants across 11 counties. All samples were tested with the dot-immunobinding assay (DIBA) against the antisera of 10 known viruses. Samples infected with papaya ringspot virus (PRSV-W), watermelon mosaic virus (WMV), zucchini yellow mosaic virus (ZYMV), and cucurbit aphid-borne-yellows virus (CABYV) were also tested by RT-PCR. Of the 10 viruses, PRSV-W was the most widespread, with an overall prevalence of 59.1%, present in all 11 counties, followed by ZYMV (27.6%), in 10 counties, and WMV (20.7%), in seven counties, while the remaining viruses were present sporadically with low incidence. Approximately 42% of the infected samples were positive, with more than one virus indicating a high proportion of mixed infections. CABYV was detected for the first time in Oklahoma, and the phylogenetic analysis of the first complete genome sequence of a CABYV isolate (BL-4) from the US showed a close relationship with Asian isolates.

scholarly journals The Complete Nucleotide Sequence of Bean Yellow Mosaic Virus Genomic RNA.

1996 ◽  
Vol 62 (5) ◽  
pp. 472-477 ◽  
Author(s):  
Shigeo NAKAMURA ◽  
Ryoso HONKURA ◽  
Takayoshi IWAI ◽  
Masashi UGAKI ◽  
Yuko OHASHI
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Complete Nucleotide Sequence ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Genomic Rna

scholarly journals PCNA Interacts with Indian Mung Bean Yellow Mosaic Virus Rep and Downregulates Rep Activity

2004 ◽  
Vol 78 (21) ◽  
pp. 11890-11903 ◽  
Author(s):  
Basavaraj Bagewadi ◽  
Shoajiang Chen ◽  
Sunil K. Lal ◽  
Nirupam Roy Choudhury ◽  
Sunil K. Mukherjee
Keyword(s):  
Mosaic Virus ◽  
Mung Bean ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Nuclear Antigen ◽  
Resting Cells ◽  
Rolling Circle Replication ◽  
Proliferative Cell Nuclear Antigen ◽  
Bipartite Genome

ABSTRACT Proliferative cell nuclear antigen (PCNA), a conserved plant protein as well as an important replication factor, is induced in response to geminivirus infection in the resting cells of the phloem tissues. The biochemical role of PCNA in rolling circle replication (RCR) of geminivirus DNA has not been explored in detail. The initiation of RCR of the bipartite genome of a geminivirus, Indian mung bean yellow mosaic virus (IMYMV), is mainly controlled by viral protein Rep (or AL1 or AC1). The role of host PCNA in RCR of IMYMV was revealed by studying the physical and functional interactions between recombinant PCNA and recombinant IMYMV Rep. Pea nuclear PCNA as well as recombinant pea PCNA showed binding to recombinant Rep in experiments involving both affinity chromatography and yeast two-hybrid approaches. The contacting amino acid residues of PCNA seemed to be present throughout a wide region of the trimeric protein, while those of Rep appeared to be localized only in the middle part of the protein. The site-specific nicking-closing activity and the ATPase function of IMYMV Rep were impaired by PCNA. These observations lead to interesting speculations about the control of viral RCR and dynamic profiles of protein-protein interactions at the RCR origin of the geminiviruses.

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