Tumor Properties Mediate the Relationship Between Peripheral Blood Monocytes and Tumor-Associated Macrophages in Breast Cancer

2021 ◽  
pp. 1-20
Author(s):  
Marina Stakheyeva ◽  
Marina Patysheva ◽  
Evgenia Kaigorodova ◽  
Marina Zavyalova ◽  
Natalia Tarabanovskaya ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Peripheral Blood ◽  
Blood Monocytes ◽  
Tumor Associated Macrophages ◽  
Peripheral Blood Monocytes ◽  
The Relationship

Expression of CD4+CD25+T Regulatory Cells and Foxp3 in Peripheral Blood of Patients with Rheumatoid Arthritis Patient

2013 ◽  
Vol 709 ◽  
pp. 844-847
Author(s):  
Ke Xin Sun ◽  
Yan Li ◽  
Su Hong Guo ◽  
Yi Ju Hou
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cells ◽  
Peripheral Blood ◽  
T Regulatory Cells ◽  
Blood Monocytes ◽  
Foxp3 Mrna ◽  
Peripheral Blood Monocytes ◽  
Polymerase Chain ◽  
The Relationship ◽  
Normal Controls

To investigate the expression of CD4+CD25+T cells and the Foxp3 in peripheral blood of rheumatoid arthritis（RA），and to analyze the relationship between their activities and patho- genesis.The number of CD4+CD25+T lymphocytes in the peripheral blood and Foxp3 mRNA expression on peripheral blood monocytes of 48 RA patients and 35 normal controls were analyzed by three-color FACs analysis and reverse transcription-polymerase chain reaction（RT- PCR）．The expression of CD4+CD25+T cells RA patients in active group was significantly lower than that in remission group and healthy controls（P0.05）； The relationship between peripheral blood CD4+CD25+Tregcells as well as the Foxp3 mRNA and active renal score was negatively correlated. The expression of CD4+CD25+Tregcells and the Foxp3 mRNA of peripheral blood in RA patients is abnormal and it is correlated with pathogenesis and activity of RA.

scholarly journals Breast cancer induces systemic immune changes on cytokine signaling in peripheral blood monocytes and lymphocytes

EBioMedicine ◽  
2020 ◽  
Vol 52 ◽  
pp. 102631 ◽  
Author(s):  
Lei Wang ◽  
Diana L. Simons ◽  
Xuyang Lu ◽  
Travis Y. Tu ◽  
Christian Avalos ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Peripheral Blood ◽  
Cytokine Signaling ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Immune Changes ◽  
Monocytes And Lymphocytes

Cytotoxicity on Tumor Cells of Peripheral Blood Monocytes and Tumor-Associated Macrophages in Patients With Ascites Ovarian Tumors23

1980 ◽  
Vol 64 (6) ◽  
pp. 1307-1315 ◽  
Author(s):  
Alberto Mantovani ◽  
Nadia Polentarutti ◽  
Giuseppe Peri ◽  
Zvi Bar Shavit ◽  
Annunciata Vecchi ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Peripheral Blood ◽  
Blood Monocytes ◽  
Tumor Associated Macrophages ◽  
Peripheral Blood Monocytes

Characterization of Monocyte-Associated Factor V

1993 ◽  
Vol 70 (02) ◽  
pp. 273-280 ◽  
Author(s):  
Janos Kappelmayer ◽  
Satya P Kunapuli ◽  
Edward G Wyshock ◽  
Robert W Colman
Keyword(s):  
Monoclonal Antibody ◽  
Light Chain ◽  
Peripheral Blood ◽  
De Novo ◽  
Factor V ◽  
Blood Monocytes ◽  
De Novo Synthesis ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Peripheral Blood Monocytes

SummaryWe demonstrate that in addition to possessing binding sites for intact factor V (FV), unstimulated peripheral blood monocytes also express activated factor V (FVa) on their surfaces. FVa was identified on the monocyte surface by monoclonal antibody B38 recognizing FVa light chain and by human oligoclonal antibodies H1 (to FVa light chain) and H2 (to FVa heavy chain) using immunofluorescence microscopy and flow cytometry. On Western blots, partially cleaved FV could be identified as a 220 kDa band in lysates of monocytes. In addition to surface expression of FVa, monocytes also contain intracellular FV as detected only after permeabilization by Triton X-100 by monoclonal antibody B10 directed specifically to the Cl domain not present in FVa. We sought to determine whether the presence of FV in peripheral blood monocytes is a result of de novo synthesis.Using in situ hybridization, no FV mRNA could be detected in monocytes, while in parallel control studies, factor V mRNA was detectable in Hep G2 cells and CD18 mRNA in monocytes. In addition, using reverse transcriptase and the polymerase chain reaction, no FV mRNA was detected in mononuclear cells or in U937 cells, but mRNA for factor V was present in Hep G2 cells using the same techniques. These data suggest that FV is present in human monocytes, presumably acquired by binding of plasma FV, and that the presence of this critical coagulation factor is not due to de novo synthesis.

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