In silico screening and epitope mapping of leptospiral outer membrane protein—Lsa46

2021 ◽  
pp. 1-19
Author(s):  
Junaida M. Ibrahim ◽  
Shanitha A. ◽  
Achuthsankar S. Nair ◽  
Oommen V. Oommen ◽  
Perumana R. Sudhakaran
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
In Silico ◽  
Epitope Mapping ◽  
In Silico Screening

scholarly journals In silico proteomic and phylogenetic analysis of the outer membrane protein repertoire of gastric Helicobacter species

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Eva Bauwens ◽  
Myrthe Joosten ◽  
Joemar Taganna ◽  
Mirko Rossi ◽  
Ayla Debraekeleer ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
In Silico

scholarly journals Effects of carbamate pesticides intermediates on Escherichia coli membrane architecture: An in vitro and in silico approach

2021 ◽  
Vol 36 (3) ◽  
pp. e2021020
Author(s):  
Pushpendra Singh ◽  
Manish Kumar Tripathi ◽  
Mohammad Yasir ◽  
Ashish Ranjan ◽  
Rahul Shrivastava
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
In Silico ◽  
Synthetic Analogue ◽  
Methyl Isocyanate ◽  
Kcal Mole ◽  
E Coli

Methyl isocyanate (MIC), a low molecular weight synthetic aliphatic compound, having an isocyanate group (−NCO), has industrial application. In this study, the effects of methyl isocyanate and its mechanism on outer membrane protein of Escherichia coli were observed using experimental and computational methods. In vitro exposure of N-succinimidyl N-methylcarbamate (NSNM) a synthetic analogue of MIC on E. coli to a final concentration of 2 mM was found to affect the growth curve pattern and changes in cell morphology. Molecular docking studies of MIC and NSNM with E. coli outer membrane protein (OmpW, OmpX, OmpF OmpA), and periplasmic domain (PAL) were performed. The in-silico results revealed that outer membrane protein OmpF showed the highest negative binding energy, i.e. ∆G -4.11 kcal/mole and ∆G -3.19 kcal/mole by NSNM and MIC as compared to other proteins. Our study concludes that methyl isocyanate retains lethal toxicity which leads to cell death due to the membrane protein damage of E. coli membrane.

scholarly journals Cross-Reactive Epitopes in Borrelia burgdorferi p66

2015 ◽  
Vol 22 (7) ◽  
pp. 840-843 ◽  
Author(s):  
Paul M. Arnaboldi ◽  
Raymond J. Dattwyler
Keyword(s):  
Lyme Disease ◽  
Membrane Protein ◽  
Borrelia Burgdorferi ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Epitope Mapping ◽  
Serum Antibody ◽  
Content Type ◽  
Linear Epitopes

ABSTRACTEpitope mapping of the p66 outer membrane protein ofBorrelia burgdorferirevealed that the protein contains numerous cross-reactive linear epitopes recognized by serum antibody in the majority of individuals tested, regardless of Lyme disease history, limiting the usefulness of this antigen in Lyme disease serodiagnostic assays.

Sign in / Sign up

Export Citation Format

Share Document