Evaluation of the Role of MHC Class II Alleles, Haplotypes and Selected Amino Acid Sequences in Primary Sclerosing Cholangitis

Autoimmunity ◽  
2002 ◽  
Vol 35 (8) ◽  
pp. 555-564 ◽  
Author(s):  
Peter T. Donaldson ◽  
Suzanne Norris
Keyword(s):  
Amino Acid ◽  
Primary Sclerosing Cholangitis ◽  
Mhc Class Ii ◽  
Sclerosing Cholangitis ◽  
Class Ii ◽  
Amino Acid Sequences ◽  
Mhc Class Ii Alleles

Molecular characterization of MHC class II DRA cDNA in Deoni cattle

2018 ◽  
Author(s):  
K. Swathi ◽  
M. Gnana Prakash ◽  
D. Sakaram ◽  
T. Raghunandan ◽  
A. Sarat Chandra ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Mhc Class Ii ◽  
Signal Peptide ◽  
Class Ii ◽  
Glycosylation Site ◽  
Amino Acid Sequences ◽  
Conserved Residues ◽  
A2 Domain

The present study was undertaken to clone and characterize DRA gene in Deoni cattle. The cDNA for the DRA gene was amplified by using specific primers designed based on available cattle sequences and purified products were cloned in competent E.coli (DH5á) strain. The full length 1013bp product of cDNA of DRA contained a single ORF of 762 nucleotides that coded for 253 amino acids translated product. Twenty four amino acids formed signal peptide while 229 constituted mature peptide. The deduced amino acid sequences resembled those of class II molecules of other species for all the conserved residues having critical functional role. But a single N-linked glycosylation site in á1 was observed in cattle and buffalo when compared to human and swine which contain a second site in á2 domain. The signal peptide was found more variable among the species compared. Comparison of nucleotide and amino acid sequences among related species and dendrogram constructed revealed that the cattle sequences are more similar to buffalo sequences.

Notes: Specificity of anti-MHC Class II Antibody Binding to Synthetic Peptides

1989 ◽  
Vol 44 (9-10) ◽  
pp. 886-889
Author(s):  
Alberto Chersi ◽  
Teresa F. Romano ◽  
Emilio Ruocco
Keyword(s):  
Amino Acid ◽  
Mhc Class Ii ◽  
Sequence Homology ◽  
Synthetic Peptides ◽  
Class Ii ◽  
Amino Acid Sequences ◽  
Beta Chain ◽  
Small Peptides ◽  
Histocompatibility Antigens ◽  
High Sequence Homology

Abstract This study indicates that antibodies raised against a DR4 , w6; DQw 1,3 positive cell line may bind to synthetic peptides selected from the polymorphic amino acid se­quences 51 -59 and 63 -79 on the DQw 2 beta chain. This cross-reaction may be explained by the relatively high sequence homology of these sequences in the beta chains of class II histocompatibility antigens, and suggests that anti­ body binding to small peptides may be scarsely selective. Based on the observations of the reactivity of the anti­ bodies with several cell lines, and comparison of the amino acid sequences of beta chains of DR and DQ molecules, an attempt to identify the cross-reacting epitope is presented.

The Role of IFNγ in the Immune Pathogenesis of Primary Sclerosing Cholangitis

2018 ◽  
Vol 56 (01) ◽  
pp. E2-E89
Author(s):  
G Ravichandran ◽  
T Krech ◽  
G Tiegs ◽  
R Barikbin
Keyword(s):  
Primary Sclerosing Cholangitis ◽  
Sclerosing Cholangitis ◽  
Immune Pathogenesis

scholarly journals Roles of the C-Terminal Amino Acids of Non-Hexameric Helicases: Insights from Escherichia coli UvrD

2021 ◽  
Vol 22 (3) ◽  
pp. 1018
Author(s):  
Hiroaki Yokota
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Amino Acid ◽  
Single Molecule ◽  
Underlying Mechanism ◽  
Amino Acid Sequences ◽  
E Coli ◽  
X Ray Crystallography ◽  
Terminal Amino

Helicases are nucleic acid-unwinding enzymes that are involved in the maintenance of genome integrity. Several parts of the amino acid sequences of helicases are very similar, and these quite well-conserved amino acid sequences are termed “helicase motifs”. Previous studies by X-ray crystallography and single-molecule measurements have suggested a common underlying mechanism for their function. These studies indicate the role of the helicase motifs in unwinding nucleic acids. In contrast, the sequence and length of the C-terminal amino acids of helicases are highly variable. In this paper, I review past and recent studies that proposed helicase mechanisms and studies that investigated the roles of the C-terminal amino acids on helicase and dimerization activities, primarily on the non-hexermeric Escherichia coli (E. coli) UvrD helicase. Then, I center on my recent study of single-molecule direct visualization of a UvrD mutant lacking the C-terminal 40 amino acids (UvrDΔ40C) used in studies proposing the monomer helicase model. The study demonstrated that multiple UvrDΔ40C molecules jointly participated in DNA unwinding, presumably by forming an oligomer. Thus, the single-molecule observation addressed how the C-terminal amino acids affect the number of helicases bound to DNA, oligomerization, and unwinding activity, which can be applied to other helicases.

scholarly journals The Role of Microbiota in Primary Sclerosing Cholangitis and Related Biliary Malignancies

2021 ◽  
Vol 22 (13) ◽  
pp. 6975
Author(s):  
Burcin Özdirik ◽  
Tobias Müller ◽  
Alexander Wree ◽  
Frank Tacke ◽  
Michael Sigal
Keyword(s):  
Primary Sclerosing Cholangitis ◽  
Gut Microbiome ◽  
Sclerosing Cholangitis ◽  
Functional Characterization ◽  
Significant Risk ◽  
Pharmacological Agents ◽  
Experimental Mouse ◽  
Biliary Malignancies ◽  
End Stage

Primary sclerosing cholangitis (PSC) is an immune-related cholangiopathy characterized by biliary inflammation, cholestasis, and multifocal bile duct strictures. It is associated with high rates of progression to end-stage liver disease as well as a significant risk of cholangiocarcinoma (CCA), gallbladder cancer, and colorectal carcinoma. Currently, no effective medical treatment with an impact on the overall survival is available, and liver transplantation is the only curative treatment option. Emerging evidence indicates that gut microbiota is associated with disease pathogenesis. Several studies analyzing fecal and mucosal samples demonstrate a distinct gut microbiome in individuals with PSC compared to healthy controls and individuals with inflammatory bowel disease (IBD) without PSC. Experimental mouse and observational human data suggest that a diverse set of microbial functions may be relevant, including microbial metabolites and bacterial processing of pharmacological agents, bile acids, or dietary compounds, altogether driving the intrahepatic inflammation. Despite critical progress in this field over the past years, further functional characterization of the role of the microbiota in PSC and related malignancies is needed. In this review, we discuss the available data on the role of the gut microbiome and elucidate important insights into underlying pathogenic mechanisms and possible microbe-altering interventions.

Role of MHC Class II Genes in the pathogenesis of pemphigoid

2011 ◽  
Vol 11 (1) ◽  
pp. 40-47 ◽  
Author(s):  
L.R. Zakka ◽  
P. Reche ◽  
A.R. Ahmed
Keyword(s):  
Mhc Class Ii ◽  
Class Ii ◽  
Mhc Class Ii Genes
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