Genetic diversity ofCucurbita maximaassessed using morphological characteristics and random-amplified polymorphic DNA markers in China

Genetic diversity of Narcissus was systematically studied on both morphological and molecular levels. Twenty-four characteristics of nine narcissi were observed and their differences evaluated by clustering method. The results showed that nine narcissi can be divided into two subclusters: one comprised by Narcissus pseudonarcissus, the other by Chinese Narcissus. The morphological diversity among five cultivars of N. pseudonarcissus is higher than that among four ecotypes of Chinese Narcissus (Narcissus tazetta var. chinensis). There are seven morphological characteristics in N. pseudonarcissus presenting obvious variations with coefficients from 33.33% to 91.67%. Only five morphological characteristics in Chinese Narcissus present certain variations with coefficients from 37.04% to 51.79%. On DNA level, two clusters are distantly related too. Based on the random amplified polymorphic DNA (RAPD) markers, 13 out of 40 random primers yielded scorable polymorphisms between samples. Wide variations in banding profiles between cultivars or between ecotypes were observed with nearly every primer tested. Among 95 band positions that were scored for all the 9 narcissi, 81 are polymorphic (85.26%). Cluster analysis of the calculated similarity matrix revealed that the genetic diversity between these individuals within the same section is low. However, the genetic diversity between two sections is obviously higher. Taken together, the methods combined morphological characteristics and RAPD technique allow a deep evaluation of the variation of Narcissus on both section level and cultivar/ecotype level.

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Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers

Indonesian Journal of Biotechnology ◽

10.22146/ijbiotech.25798 ◽

2018 ◽

Vol 22 (1) ◽

pp. 22

Author(s):

Jayusman Jayusman ◽

Muhammad Na’iem ◽

Sapto Indrioko ◽

Eko Bhakti Hardiyanto ◽

ILG Nurcahyaningsih

Keyword(s):

Genetic Diversity ◽

Dna Markers ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Management Strategies ◽

Genetic Distances ◽

Sampled Data ◽

Toona Sinensis ◽

The Mean ◽

Individual Trees

Surian Toona sinensis Roem is one of the most widely planted species in Indonesia. This study aimed to estimate the genetic diversity between a number of surian populations in a progeny test using RAPD markers, with the goal of proposing management strategies for a surian breeding program. Ninety-six individual trees from 8 populations of surian were chosen as samples for analysis. Eleven polymorphic primers (OP-B3, OP-B4, OP-B10, OP-H3, OP-Y6, OP-Y7, OP-Y8, OP-Y10, OP-Y11, OP-Y14, and OP-06) producing reproducible bands were analyzed for the 96 trees, with six trees per family sampled. Data were analyzed using GenAlEx 6.3, NTSYS 2.02. The observed percentage of polymorphic loci ranged from 18.2% to 50%. The mean level of genetic diversity among the surian populations was considered to be moderate (He 0.304). Cluster analysis grouped the genotypes into two main clusters, at similarity levels of 0.68 and 0.46. The first two axes of the PCoA explained 46.16% and 25.54% of the total variation, respectively. The grouping of samples into clusters and subclusters did not correspond with family and their distances, but the grouping was in line with the genetic distances of the samples.

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Distribution of Mating Types and Genetic Diversity of Ascochyta rabiei Populations in Tunisia Revealed by Mating-type-specific PCR and Random Amplified Polymorphic DNA Markers

Journal of Phytopathology ◽

10.1111/j.1439-0434.2007.01285.x ◽

2007 ◽

Vol 155 (10) ◽

pp. 596-605 ◽

Cited By ~ 7

Author(s):

A. Rhaiem ◽

M. Cherif ◽

P. S. Dyer ◽

T. L. Peever

Keyword(s):

Genetic Diversity ◽

Mating Type ◽

Dna Markers ◽

Random Amplified Polymorphic Dna ◽

Ascochyta Rabiei ◽

Mating Types ◽

Specific Pcr

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Use of Random Amplified Polymorphic DNA Markers for Cultivar Identification in Mint

HortScience ◽

10.21273/hortsci.36.4.761 ◽

2001 ◽

Vol 36 (4) ◽

pp. 761-764 ◽

Cited By ~ 15

Author(s):

A.L. Fenwick ◽

S.M. Ward

Keyword(s):

United States ◽

Genetic Diversity ◽

Dna Markers ◽

Geographical Origin ◽

Cultivar Identification ◽

Rapd Analysis ◽

Random Amplified Polymorphic Dna ◽

The United States ◽

Randomly Amplified Polymorphic Dna ◽

Commercial Oil

Seventeen mint accessions representing the three species grown for commercial oil production in the United States were characterized using randomly amplified polymorphic DNA (RAPD) analysis. The RAPD profiles readily identified the different Mentha species; calculation of genetic distance, based on the number of shared bands, indicated that M. spicata L. is more closely related to M. × gracilis than to M. × piperita. The RAPD profiles also distinguished among eight peppermint accessions of different geographical origin. However, only limited polymorphism was observed among the most widely grown peppermint and Scotch spearmint cultivars. These results indicate a potential lack of genetic diversity in mint cultivars grown for oil in the United States.

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