scholarly journals Production of styrene oxide from styrene by a recombinant Escherichia coli with enhanced AcrAB-TolC efflux pump level in an aqueous-organic solvent two-phase system

2020 ◽  
Vol 84 (7) ◽  
pp. 1513-1520
Author(s):  
Noriyuki Doukyu ◽  
Shinichiro Iida
Keyword(s):  
Escherichia Coli ◽  
Organic Solvent ◽  
Efflux Pump ◽  
Styrene Oxide ◽  
Recombinant Escherichia Coli ◽  
Phase System ◽  
Pump Level ◽  
Two Phase

An aqueous two-phase system to pre-purify a heterologously produced siderophore

TECHNOLOGY ◽  
2016 ◽  
Vol 04 (03) ◽  
pp. 135-138
Author(s):  
Mahmoud Kamal Ahmadi ◽  
Samar Fawaz ◽  
Blaine A. Pfeifer
Keyword(s):  
Escherichia Coli ◽  
Natural Products ◽  
Cell Growth ◽  
Complex Nature ◽  
Phase System ◽  
Heterologous Production ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Purification System ◽  
Surrogate Host

Natural products span broad activities and applications; however, their access and production are often limited by native cellular sources. As a result, the heterologous production of a siderophore termed yersiniabactin (Ybt) was completed using the surrogate host Escherichia coli. Post-production and purification steps are complicated by the complex nature of most media used for cell growth, prompting the development in this work of an aqueous two-phase pre-purification system capable of rapidly and simply enhancing the concentration of the target Ybt compound.

scholarly journals Oxidative Bioconversion of Cholesterol by Pseudomonas sp. Strain ST-200 in a Water-Organic Solvent Two-Phase System

1994 ◽  
Vol 60 (7) ◽  
pp. 2518-2523 ◽  
Author(s):  
Rikizo Aono ◽  
Noriyuki Doukyu ◽  
Hideki Kobayashi ◽  
Harushi Nakajima ◽  
Koki Horikoshi
Keyword(s):  
Organic Solvent ◽  
Phase System ◽  
Pseudomonas Sp ◽  
Two Phase

One-step purification of histone deacetylase from Escherichia coli cell-lysate by counter-current chromatography using aqueous two-phase system

2007 ◽  
Vol 1151 (1-2) ◽  
pp. 158-163 ◽  
Author(s):  
Yoichi Shibusawa ◽  
Naoko Takeuchi ◽  
Kanako Tsutsumi ◽  
Shigeru Nakano ◽  
Akio Yanagida ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Histone Deacetylase ◽  
Coli Cell ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Cell Lysate ◽  
One Step ◽  
Counter Current

Extraction of plasmid DNA from Escherichia coli cell lysate in a thermoseparating aqueous two-phase system

2004 ◽  
Vol 1024 (1-2) ◽  
pp. 95-104 ◽  
Author(s):  
Cecilia Kepka ◽  
Jenny Rhodin ◽  
Raf Lemmens ◽  
Folke Tjerneld ◽  
Per-Erik Gustavsson
Keyword(s):  
Escherichia Coli ◽  
Plasmid Dna ◽  
Coli Cell ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Cell Lysate
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