Extraction of plasmid DNA from Escherichia coli cell lysate in a thermoseparating aqueous two-phase system

2004 ◽  
Vol 1024 (1-2) ◽  
pp. 95-104 ◽  
Author(s):  
Cecilia Kepka ◽  
Jenny Rhodin ◽  
Raf Lemmens ◽  
Folke Tjerneld ◽  
Per-Erik Gustavsson
Keyword(s):  
Escherichia Coli ◽  
Plasmid Dna ◽  
Coli Cell ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Cell Lysate

One-step purification of histone deacetylase from Escherichia coli cell-lysate by counter-current chromatography using aqueous two-phase system

2007 ◽  
Vol 1151 (1-2) ◽  
pp. 158-163 ◽  
Author(s):  
Yoichi Shibusawa ◽  
Naoko Takeuchi ◽  
Kanako Tsutsumi ◽  
Shigeru Nakano ◽  
Akio Yanagida ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Histone Deacetylase ◽  
Coli Cell ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Cell Lysate ◽  
One Step ◽  
Counter Current

An aqueous two-phase system to pre-purify a heterologously produced siderophore

TECHNOLOGY ◽  
2016 ◽  
Vol 04 (03) ◽  
pp. 135-138
Author(s):  
Mahmoud Kamal Ahmadi ◽  
Samar Fawaz ◽  
Blaine A. Pfeifer
Keyword(s):  
Escherichia Coli ◽  
Natural Products ◽  
Cell Growth ◽  
Complex Nature ◽  
Phase System ◽  
Heterologous Production ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Purification System ◽  
Surrogate Host

Natural products span broad activities and applications; however, their access and production are often limited by native cellular sources. As a result, the heterologous production of a siderophore termed yersiniabactin (Ybt) was completed using the surrogate host Escherichia coli. Post-production and purification steps are complicated by the complex nature of most media used for cell growth, prompting the development in this work of an aqueous two-phase pre-purification system capable of rapidly and simply enhancing the concentration of the target Ybt compound.

Pilot-scale extraction of an intracellular recombinant cutinase from E. coli cell homogenate using a thermoseparating aqueous two-phase system

2003 ◽  
Vol 103 (2) ◽  
pp. 165-181 ◽  
Author(s):  
Cecilia Kepka ◽  
Eric Collet ◽  
Josefine Persson ◽  
Åke Ståhl ◽  
Torgny Lagerstedt ◽  
...  
Keyword(s):  
Pilot Scale ◽  
Coli Cell ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
E Coli ◽  
Cell Homogenate

scholarly journals A Combined Aqueous Two-Phase System and Spot-Test Platform for the Rapid Detection of Escherichia coli O157:H7 in Milk

2017 ◽  
Vol 23 (1) ◽  
pp. 57-63 ◽  
Author(s):  
Sherine F. Cheung ◽  
Matthew F. Yee ◽  
Nguyen K. Le ◽  
Elizabeth A. Gomes ◽  
Zahra Afrasiabi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
Potassium Phosphate ◽  
The United States ◽  
Spot Test ◽  
Health Concern ◽  
Phase System ◽  
Escherichia Coli O157 ◽  
Two Phase ◽  
Aqueous Two Phase System

Foodborne illnesses are a public health concern in the United States and worldwide. Recent outbreaks of Escherichia coli O157:H7 have brought to light the need for improved ways to detect foodborne pathogens and minimize serious outbreaks. Unfortunately, current methods for the detection of foodborne pathogens are time intensive and complex. In this study, we designed a spot immunoassay that uses a UCON-potassium phosphate salt aqueous two-phase system (ATPS) for the preconcentration of O157:H7. This platform was tested with samples of O157:H7 spiked in phosphate-buffered saline and milk. The ATPS was found to improve the detection limit of the spot test, yielding detection at 106 cfu/mL within 30 min. This is the first known application of ATPSs to spot immunoassays. Moreover, detection was successfully achieved without upstream processing or dilution of the sample prior to testing, thereby further simplifying the detection process. This technology’s ease of use, sensitivity, and short time to result highlight its potential to advance the spot test as a viable diagnostic tool for foodborne pathogens.

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