scholarly journals Establishment of a monoclonal antibody-based indirect enzyme-linked immunosorbent assay for the detection of trimethoprim residues in milk, honey, and fish samples

2016 ◽  
Vol 27 (6) ◽  
pp. 830-840 ◽  
Author(s):  
Yanni Chen ◽  
Liqiang Liu ◽  
Shanshan Song ◽  
Hua Kuang ◽  
Chuanlai Xu
2006 ◽  
Vol 13 (3) ◽  
pp. 420-422 ◽  
Author(s):  
S. E. Burastero ◽  
C. Paolucci ◽  
D. Breda ◽  
G. Monasterolo ◽  
R. E. Rossi ◽  
...  

ABSTRACT The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcεRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.


2010 ◽  
Vol 120 (4) ◽  
pp. 1178-1184 ◽  
Author(s):  
Yuanyuan Xia ◽  
Qing X. Li ◽  
Shuangjun Gong ◽  
Yong Li ◽  
Yongsong Cao ◽  
...  

1987 ◽  
Vol 82 (1) ◽  
pp. 81-85 ◽  
Author(s):  
Mauro Schechter

Theoretically, serological assays with affinity purified marker antigens can allow strain-specific diagnosis even when parasites cannot be retrieved from and infected host. A Trypanosoma cruzi antigen was purified by affinity chromatography using a zymodeme (Z) 2 specific monoclonal antibody (2E2C11). An indirect enzyme-linked immunosorbent assay (ELISA) based on the purified antigen could discriminate between sera from rabbits immunized with T. cruzi zymodeme clones but could not discriminate between sera from mice infected with different zymodemes.


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