Utilization of waste glycerol for the production of biocontrol agents nigericin and niphimycin by Streptomyces hygroscopicus: bioprocess development

2021 ◽  
pp. 1-14
Author(s):  
Ivana Mitrović ◽  
Jovana Grahovac ◽  
Jovana Hrustić ◽  
Aleksandar Jokić ◽  
Jelena Dodić ◽  
...  
Keyword(s):  
Biocontrol Agents ◽  
Streptomyces Hygroscopicus ◽  
Bioprocess Development ◽  
Waste Glycerol

scholarly journals Production of plant protection agents in medium containing waste glycerol by Streptomyces hygroscopicus: Bioprocess analysis

2020 ◽  
Vol 49 (3) ◽  
pp. 270-277
Author(s):  
I. Mitrović ◽  
J. Grahovac ◽  
J. Dodić ◽  
A. Jokić ◽  
Z. Rončević ◽  
...  
Keyword(s):  
Plant Protection ◽  
Phytopathogenic Fungi ◽  
Global Market ◽  
Biodiesel Production ◽  
Streptomyces Hygroscopicus ◽  
Diffusion Method ◽  
Agitation Rate ◽  
Antifungal Metabolites ◽  
Waste Glycerol ◽  
Fruit Samples

The surplus of waste glycerol, by-product of the biodiesel production process, is available at the global market. Some species of the genera Streptomyces have the ability to assimilate glycerol and convert it into valuable metabolic products. In the present study, the ability of Streptomyces hygroscopicus to assimilate waste glycerol and convert it into metabolic compounds with antifungal activity against four phytopathogenic fungi obtained from apple fruit samples expressing rot symptoms, was investigated. Production of antifungal metabolites by S. hygroscopicus was carried out in 3 l stirred tank bioreactor through 7 days. Fermentation was carried out at 27 °C with aeration rate of 1.5 vvm and agitation rate of 100 r.p.m. The aim of this work was to analyse bioprocess parameters and to determine at which stage of bioprocess the production of antifungal metabolites occurs. Activity of the cultivation liquid on two isolates of Alternaria alternata and two isolates of Fusarium avenaceum were determined every 12 h using in vitro well diffusion method. It was found that the maximum production of antifungal metabolites occurred at 108 hour of cultivation. Formed inhibition zones have shown that the produced antifungal metabolites have high efficacy on tested phytopathogenic fungi (inhibition zone diameter higher than 35 mm for all test organisms).

Plants as biocontrol agents against phytoparasite nematodes

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
P Avato ◽  
M Argentieri ◽  
S Laquale ◽  
T D'Addabbo
Keyword(s):  
Biocontrol Agents

Exploration of Endophytes from Alfalfa (Medicago sativa L.) as Biocontrol Agents

2020 ◽  
pp. 1-8
Author(s):  
Beilei Wu ◽  
Beilei Wu ◽  
Mei Li ◽  
Xiaoli Chen ◽  
Xiliang Jiang ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Root Rot ◽  
Agricultural Production ◽  
Biocontrol Agents ◽  
Bacillus Spp ◽  
Medicago Sativa L ◽  
The Common ◽  
Seedling Germination ◽  
Germination Experiments ◽  
Alfalfa Root

Endophytes are increasingly investigated as biocontrol agents for agricultural production. The identification of new endophytes with high effectiveness against plant disease is very important. A total of 362 strains of endophytes, including fungi, bacteria, and actinomycete, were isolated from alfalfa (Medicago sativa L.) collected in Hebei, Inner Mongolia and Ningxia provinces of China. The three strains of endophytic bacteria (NA NX51R-5, NA NX90R-8, and NA NM1S-1) with strong biocontrol capability with >50% effectiveness were screened against the common alfalfa root rot pathogen Fusarium oxysporum F. sp. medicaginis in alfalfa seedling germination experiments on MS medium and pot experiments. Using phylogenetic analysis, the isolates of NA NM1S-1 and NA NX51R-5 were identified as Bacillus spp. by 16S rDNA, while NA NX90R-8 was found to be Pseudomonas sp.

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