A new CSN3 allele in Bos indicus cattle is characterised by Msp I PCR-RFLP

1999 ◽  
Vol 30 (2) ◽  
pp. 164-164 ◽  
Author(s):  
E-M. Prinzenberg ◽  
G. Erhardt
Keyword(s):  
Bos Indicus ◽  
Pcr Rflp

Studies on TLR2 gene variants and their association with milk yield and milk quality traits in Bos indicus (Deoni) cattle

2017 ◽  
Author(s):  
U. T. Mundhe ◽  
D. N. Das ◽  
R. S. Gandhi ◽  
P. Divya
Keyword(s):  
Milk Yield ◽  
Bos Indicus ◽  
Restriction Enzymes ◽  
Milk Quality ◽  
Quality Traits ◽  
Exon 2 ◽  
Allelic Frequencies ◽  
Tlr2 Gene ◽  
Pcr Rflp

Present study molecular characterization of exon 2 of TLR2 gene and its association with milk yield and milk quality traits in 104 Deoni cattle using PCR- RFLP technique was done. Polymorphism was observed through HaeIII, HhaI and EcoRV restriction enzymes in Created Restriction Site (CRS) exon 2-1, CRS exon 2-5 and exon 2-1 by PCR- RFLP, respectively. In CRS exon 2-1 allelic frequencies were observed as 0.793 for A and 0.206 for B alleles and that of genotypic frequencies were 0.58 and 0.41 for genotypes AA and AB. In CRS exon 2-5, two genotypes viz., AC and CC with corresponding allelic frequencies were observed as 0.221 for A and 0.778 for C allele and that of genotypic frequencies observed were 0.44 and 0.55 for AC and CC genotypes respectively. TLR2 exon 2-1 exhibited two alleles G and T with frequencies of 0.134 and 0.865 and their Corresponding genotypic frequencies were 0.009, 0.25 and 0.74for GG, GT and TT genotypes respectively. Higher count of somatic cells (SCC) in TT homozygous and TG heterozygous genotypes, and lower in GG homozygous genotypes were observed in exon 2-1. Strongly significant (P£0.01) effect for least squares means of Test Day milk yield (TDMY) and Somatic Cell Count of CRS exon 2-1 were observed.

scholarly journals Genetic Variation of Calpastation Gene of Indigenous Bali Cattle (Bos javanicus) in Indonesia

2019 ◽  
Vol 26 (1) ◽  
pp. 44
Author(s):  
Endang Tri Margawati ◽  
Slamet Diah Volkandari ◽  
Indriawati Indriawati ◽  
Emma M. Svensson
Keyword(s):  
Genetic Variation ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Meat Tenderness ◽  
Gene Markers ◽  
Bos Javanicus ◽  
Restriction Fragment Polymorphism ◽  
Pcr Rflp ◽  
Bali Cattle ◽  
Cast Gene

Calpastatin is one of gene markers affecting meat tenderness. The study aimed to evaluate genetic variation of calpastatin (CAST) gene of Bali cattle (Bos javanicus) in lndonesia. A total of 61 samples consisting of 21 Bali cattle, 22 Ongole cattle (Bos indicus), and 18 Friesian Holstein (FH) cattle (Bos taurus) were applied. The Ongole and FH cattle were involved for breed comparison. DNA was extracted from fresh blood using a High Salt method and measured their quality by a Spectrophotometer. A 523 bp of Calpastatin gene fragment was amplified by Polymerase Chain Reaction and Restriction Fragment Polymorphism (PCR-RFLP) technique with RsaI restriction enzyme for genotyping. Result showed that two variants alleles (C and G) and three genotypes (CC, GC, GG) were found in those Bali, Ongole and FH samples. Allele G was dominant allele with the highest G allele was in Bali cattle population (0.88). The higher percentage of allele C was found in Ongole and Friesian Holstein compared to that in Bali cattle. The Ongole breed tends to have a potential source of lean meat quality. This finding identified that genetic variation of CAST gene was exist in Bali cattle and adapted cattle of Ongole and FH in Indonesian.

Genetic Variants of β-casein Gene in Indigenous and Exotic Dairy Cattle Breeds in Sri Lanka

2020 ◽  
Author(s):  
S. Wickramasinghe ◽  
R. K. Rupasinghe ◽  
N. Shanjayan ◽  
L. G.S. Lokugalappatti
Keyword(s):  
Sri Lanka ◽  
Bos Indicus ◽  
Bovine Milk ◽  
Cattle Population ◽  
Health Issues ◽  
Genotype Frequencies ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium ◽  
White Cattle

Background: Bovine milk contains two types of β-casein (CSN2); A1 and A2, in which A1 type has shown associations with certain health issues. This study was conducted to identify the abundance of CSN2 A1/A2 variants in European and indigenous dairy breeds in Sri Lanka. Methods: The study included 123 Ayrshire, 101 Holstein Friesian and 92 indigenous White cattle (Bos indicus) populations. Genotyping was performed by PCR-RFLP method using Dde1. Allele and genotype frequencies and population genetic indices were calculated. Result: Results showed A1A2 to be predominant in both Holstein Friesian (0.63) and Ayrshire (0.68) populations while A2A2 (0.82) was predominant in the White cattle population. A1 and A2 allele frequencies were 0.52 and 0.48 in Ayrshire; 0.39 and 0.61 in Holstein Friesian and 0.09 and 0.91 in White cattle populations. Ayrshire and Holstein Friesian populations showed significant deviation from the Hardy-Weinberg equilibrium (p£0.05). When compared to the imported breeds, the White cattle population showed inbreeding and less genetic diversity.

DNA Polymorphism of BoLA-DRB3.2 Gene in Tharparkar Cattle (Bos indicus) by PCR-RFLP

2007 ◽  
Vol 32 (2) ◽  
pp. 187-190
Author(s):  
Bharat Bhushan ◽  
B. N. Patra ◽  
Chandan Paswan ◽  
Umang ◽  
Pushpendra Kumar ◽  
...  
Keyword(s):  
Dna Polymorphism ◽  
Bos Indicus ◽  
Tharparkar Cattle ◽  
Pcr Rflp

scholarly journals Genotyping of the polymorphism within exon 3 of prolactin gene in various dairy breeds by PCR RFLP (Brief report)

2008 ◽  
Vol 51 (3) ◽  
pp. 298-299 ◽  
Author(s):  
A. Ratna Kumari ◽  
K. M. Singh ◽  
K. J. Soni ◽  
R. K. Patel ◽  
J. B. Chauhan ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Dairy Cattle ◽  
Genetic Characterization ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Genotype Frequencies ◽  
Prolactin Gene ◽  
Pcr Rflp ◽  
Transition Mutation

Abstract. In mammals, especially dairy cattle the prolactin has important functions like the development of mammary gland affecting milk yield and composition. It has been mapped to chromosome 23 in Bovine (HALLERMAN et al., 1988). A silent A→G transition mutation at the codon for amino acid 103 in exon 3 of bovine prolactin (bPRL) gene gives rise to a polymorphic Rsa I site, has become a popular genetic marker used for genetic characterization of cattle populations by means of PCR-RFLP (MITRA et al., 1995; CHRENEK et al., 1998; DYBUS, 2005). The present study reports on the genotype frequencies observed in various Bos taurus and Bos indicus dairy cattle breeds.

Cloning, characterization and identification of polymorphism in TCR Zeta Gene in deoni cattle

2018 ◽  
Author(s):  
K. Swathi ◽  
M. Gnana Prakash ◽  
D. Sakaram ◽  
T. Raghunandan ◽  
A. Sarat Chandra ◽  
...  
Keyword(s):  
T Cell Receptor ◽  
Fragment Length Polymorphism ◽  
Bos Indicus ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Cell Receptor ◽  
Chain Reaction ◽  
Reading Frame ◽  
Pcr Rflp ◽  
Polymerase Chain

The cDNA encoding, T-cell receptor zeta (TCR z; CD247) molecule of Deoni cattle (Bos indicus), was isolated, cloned and sequenced in the present study. The CD247 cDNA comprised 1078 nucleotides including a 30 nucleotide 5¹-untranslated region (UTR), 495 nucleotide single open reading frame (ORF) and 553 nucleotide 3¹-UTR. Deduced amino acid of cattle CD247 sequence was two residues shorter than the corresponding sheep sequences. However, ruminant-specific insertions and substitutions in transmembrane (TM) and intra-cytoplasmic (IC) domain were present in cattle. Immunoreceptor tyrosine-based activation motifs (ITAMs), the important motifs for TCR signalling, were totally conserved among ruminants including cattle. The 3¹ - UTR region of the cattle CD247 was highly homologous to the corresponding region in the buffalo sequence and showed lack of polymorphism after polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using Hae III and Mse I restriction enzymes in cattle population. Phylogenetically, cattle sequence was closer to buffalo sequence under the ruminant’s lineage. The conserved nature of this gene ensures TCR integrity which is vital for induction of optimal and efficient immune response.

Sequence characterization and SNP identification of TNP1 gene in Indian cattle breeds

2017 ◽  
Author(s):  
Ashish Ranjan ◽  
K. Raja ◽  
Ranjana Sinha ◽  
I. Ganguly ◽  
I. Gupta ◽  
...  
Keyword(s):  
Promoter Region ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Coding Region ◽  
Sequence Characterization ◽  
Holstein Breed ◽  
Cattle Breeds ◽  
Pcr Rflp ◽  
Pcr Products ◽  
Indian Cattle

Present study was conducted on 50 bulls and 40 male calves of Sahiwal, Tharparkar and Karan Fries cattle maintained at ABRC and LRC, NDRI Karnal (Haryana) to characterize and identify genetic polymorphisms in TNP1 gene. A total of 1568 bp region of TNP-1 gene includes 490 bp of promoter region and two exon and one intron was sequenced and characterized in Bos indicus cattle breeds which are widely distributed in Indian sub-continent. Four sets of primers for TNP1 gene on the basis of Bos Taurus sequence (Acc. No- BK_006511) were designed using Primer3 software and PCR products of 487, 450, 455 and 250 bp were obtained. Amplicons were custom sequenced and subjected to Clustal W analysis which showed no nucleotide changes in coding region and non coding region in Indian cattle breeds as compared to Bos taurus. The 490 bp of promoter region was subjected to transcription factor binding site. Three TATA boxes and two CAAT boxes were identified in the studied fragment. Analysis of SNP was performed using restriction fragment length polymorphism (PCR-RFLP), to detect nucleotide changes in the sequence as reported (g.528G>A, SS1388116558) in Chinese Holstein breed. No polymorphisms were found for tested SNP. Only one genotype GG indicates the absence of variability in the sampled population.

scholarly journals Comparison of DNA Polymorphism of Bovine Pituitary-Specific Transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle Using PCR-RFLP

2010 ◽  
Vol 9 (11) ◽  
pp. 1660-1663
Author(s):  
Reza Nahavandi ◽  
Nader Asadzadeh ◽  
Adbdolreza Soleimani ◽  
S.M. Nurul Amin ◽  
Parivash Hafezamini ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Polymorphism ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Specific Transcription Factor ◽  
Pcr Rflp

scholarly journals Alternative genotyping method for the single nucleotide polymorphism A2959G (AF159246) of the bovine CAST gene

2008 ◽  
Vol 43 (5) ◽  
pp. 657-659 ◽  
Author(s):  
Rogério Abdallah Curi ◽  
Luis Artur Loyola Chardulo ◽  
Antônio Carlos Silveira ◽  
Henrique Nunes de Oliveira
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Bos Taurus ◽  
Direct Sequencing ◽  
Bos Indicus ◽  
Basic Structure ◽  
Meat Tenderness ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Pcr Rflp ◽  
Cast Gene

The objective of this work was to genotype the single nucleotide polymorphism (SNP) A2959G (AF159246) of bovine CAST gene by PCR-RFLP technique, and to report its use for the first time. For this, 147 Bos indicus and Bos taurus x Bos indicus animals were genotyped. The accuracy of the method was confirmed through the direct sequencing of PCR products of nine individuals. The lowest frequency of the meat tenderness favorable allele (A) in Bos indicus was confirmed. The use of PCR-RFLP for the genotyping of the bovine CAST gene SNP was shown to be robust and inexpensive, which will greatly facilitate its analysis by laboratories with basic structure.

scholarly journals ASSOCIATON OF KAPPA-CASEIN GENOTYPE AND THE LINEAR PARAMETER IN TWO INDIGENIOUS BOS INDICUS AND BOS TAURUS CATTLE IN NIGERIA

2020 ◽  
Vol 1 (1) ◽  
pp. 12-22
Author(s):  
B. M. Olanrewaju ◽  
E. B. Oghate ◽  
A. J. Adetunb ◽  
O. OlawaleJ ◽  
A. C Chineke
Keyword(s):  
Bos Taurus ◽  
Bos Indicus ◽  
The Body ◽  
Cow Milk ◽  
Physiological Processes ◽  
University Of Technology ◽  
Body Conformation ◽  
Pcr Rflp ◽  
Pcr Products ◽  
White Fulani

Kappa-casein as a mammalian milk protein is involved in a several important physiological processes and it’s about 80% of the total protein in cow milk. This study aimed at genotyping bovine Kappa casein (CSN3) in two indigenous Nigerian cattle populations and to determine the frequency distribution of Kappa casein variants as detected across the animals examined and their association with the body measurement. DNA was extracted from 100 blood samples of 50 White Fulani and 50 N’dama cattle for identification and genotyping of kappa-casein gene by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) test using HindIII restriction endonucleases. The PCR products of the specific primer K-F and K-R for the two cattle breeds give 530bp specific band. Digestion of 530bp amplified products of White Fulani and N’dama by restriction endonuclease HindIII generated three fragments of 530-, 370- and 160- bp each for the two breeds. Results of the cuts with this enzyme show the presence of genotypes AA, AB and BB in the samples. These findings suggest that BB genotype could be selected for increase body conformation and protein content of milk. Olanrewaju, B. M. | Department of Animal Production and Health, The Federal University of Technology Akure (FUTA), Ondo State, Nigeria.

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