Separation and Purification of Four Flavan-3-ols FromIris LacteaPall.var. Chinensis(Fisch.) Koidz by High-Speed Counter-Current Chromatography with Flow-Rate Gradient

Acanthopanax sessiliflorus (Rupr. & Maxim.) Seem. (Araliaceae) is one of the most abundant species of genus Acanthopanax. The fruits of A. sessiliflorus are used in traditional medical protocols as an analgesic, tonic, antidiabetic, antihypertensive, anti-inflammatory, antitumor, and immune-stimulating agent. In this work, we carried out a comprehensive investigation into the anthocyanin components in the fruits of A. sessiliflorus. The anthocyanin content in the fresh fruits of A. sessiliflorus was determined by high performance liquid chromatography-diode array detection (HPLC/DAD), and the anthocyanin component was isolated from these using high-speed counter-current chromatography (HSCCC) and elucidated by electro-spray ionization-mass spectrometry (ESI/MS), 1H- and 13C-NMR. Its antioxidant activity was evaluated by ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). We found that A. sessiliflorus contained a gross anthocyanin content of 121.35 mg/100 g. HSCCC was successfully used for separation and purification of the primary anthocyanin component, cyanidin 3-xylosyl-galactoside. The antioxidant and radical scavenging tests indicated that cyanidin 3-xylosyl-galactoside is a potent antioxidant.

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One-step separation and purification of 3,4-dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography

Journal of Chromatography A ◽

10.1016/j.chroma.2006.11.057 ◽

2007 ◽

Vol 1140 (1-2) ◽

pp. 107-111 ◽

Cited By ~ 44

Author(s):

Ming Gu ◽

Xiaolei Wang ◽

Zhiguo Su ◽

Fan Ouyang

Keyword(s):

High Speed ◽

Salvia Miltiorrhiza ◽

Salvianolic Acid B ◽

Separation And Purification ◽

Salvia Miltiorrhiza Bunge ◽

Salvianolic Acid ◽

One Step ◽

Counter Current

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Purification of Flavonoids from Mulberry Leaves via High-Speed Counter-Current Chromatography

Processes ◽

10.3390/pr7020091 ◽

2019 ◽

Vol 7 (2) ◽

pp. 91 ◽

Cited By ~ 3

Author(s):

Pian Zhang ◽

Kang-Ling Zhu ◽

Jun Zhang ◽

Yan Li ◽

Heng Zhang ◽

...

Keyword(s):

Flow Rate ◽

Mobile Phase ◽

High Speed ◽

Separation Efficiency ◽

Phase Flow ◽

Mobile Phase Flow Rate ◽

Mulberry Leaves ◽

Mulberry Leaf ◽

Solvent Systems ◽

Counter Current

In order to obtain high-purity flavonoid products, the extracts from mulberry leaves were separated and purified via high-speed counter-current chromatography (HSCCC). Moreover, the product was detected via high-performance liquid chromatography (HPLC). The characteristic absorption wavelength of the rutin standard for HSCCC detection and HPLC analysis at 257 nm was tested by ultraviolet scanning analysis. The effect of solvent systems and mobile phase flow rate on the separation efficiency were then researched. Finally, the solvent system of V(ethyl acetate):V(n-butanol):V(water) = 4:1:5 was selected as the operating system for HSCCC. This work theoretically analyzed the impact of the molecular structure and polarity of flavonoids on the choice of solvent systems. The results showed that the mobile phase flow rate had a great influence on the separation efficiency. Furthermore, the separation efficiency increased as the mobile phase flow rate decreased. When the mobile phase flow rate was 5 mL/min, the peak time for flavonoids was 140 min, the retention of the stationary phase was 56.4%, and the purity of the product reached 93.8%. The results of this study greatly improved the purity of flavonoids in mulberry leaf and provided a strong support for the separation and purification of mulberry leaf extract.

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