Rapid and sensitive determination of shikonin and its derivatives in the roots of Arnebia euchroma (Royle) Johnst using matrix solid-phase dispersion extraction and ultrahigh-performance liquid chromatography with photodiode array detector

2018 ◽  
Vol 41 (9) ◽  
pp. 489-497 ◽  
Author(s):  
Qi Zhang ◽  
Defu Cai ◽  
Liang Wang ◽  
Xueting Yang ◽  
Songjie Fan ◽  
...  
Keyword(s):  
Solid Phase ◽  
Array Detector ◽  
Matrix Solid Phase Dispersion ◽  
Phase Dispersion ◽  
Arnebia Euchroma ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Sensitive Determination ◽  
Ultrahigh Performance Liquid Chromatography

Determination of Niacin in Infant Formula by Solid-phase Clean-up and HPLC with Photodiode Array Detector

2009 ◽  
Vol 38 (3) ◽  
pp. 359-363
Author(s):  
Jee-Eun Hong ◽  
Mi-Ran Kim ◽  
Sang-Hee Cheon ◽  
Jung-Young Chai ◽  
Eun-Ryong Park ◽  
...  
Keyword(s):  
Infant Formula ◽  
Solid Phase ◽  
Array Detector ◽  
Photodiode Array Detector ◽  
Photodiode Array

scholarly journals Simultaneous Determination of Eleven Dyes and Their Aluminum Lakes in Drugs

2003 ◽  
Vol 86 (2) ◽  
pp. 215-221 ◽  
Author(s):  
Fusako Ishikawa ◽  
Sutemi Shigeoka ◽  
Machiko Nagashima ◽  
Misako Takahashi ◽  
Hisashi Kamimura
Keyword(s):  
Simultaneous Determination ◽  
Solid Phase ◽  
Indigo Carmine ◽  
Package Insert ◽  
Absorption Spectrometry ◽  
Array Detector ◽  
Organic Layer ◽  
Photodiode Array Detector ◽  
Photodiode Array

Abstract A 3-step extraction method was developed for the simultaneous determination of 11 dyes and their aluminum lakes in drugs. The dyes were first extracted with warm water (approximately 60°C) and were cleaned up by solid-phase extraction with a tC18 cartridge. Aluminum lake dyes that remained in the precipitate were extracted with 0.02M NaOH. Aluminum in the dye lakes was reextracted into the organic layer with acetylacetone–butyl acetate (1 + 9, v/v), as an acetylacetone chelate, and was quantified by atomic absorption spectrometry. The dye portions of the aluminum lakes remained in the aqueous layer and were cleaned up in the same way as the dyes. The dyes and the dye portions of the aluminum lakes were quantified by ion–pair liquid chromatography with a photodiode array detector within 20 min. The recoveries of dyes from drug fortified at 10 μg of each dye per pill were 87.0–102.2%, and the recoveries of dyes from drugs fortified at 50 μg of each dye lake per pill were 82.9–101.6%, except for recoveries of indigo carmine. In 40 ethical and over-the-counter drugs, dyes that were not indicated in the package insert information for drugs were detected in 5 samples. The highest amount of dye found in a drug was 1169.5 μg erythrosine, which was detected in a capsule of antibiotic. Aluminum lake dyes were detected in 8 samples of various dosage forms.

scholarly journals Ultrasound-assisted Matrix Solid Phase Dispersion for the HPLC-DAD analysis of amphenicols in shrimps

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Victoria F. Samanidou ◽  
Evgenia A. Makrygianni
Keyword(s):  
Solid Phase ◽  
Extraction Procedure ◽  
Array Detector ◽  
The European Union ◽  
Matrix Solid Phase Dispersion ◽  
Phase Dispersion ◽  
Cleanup Efficiency ◽  
Photodiode Array Detector ◽  
Ultrasound Assisted ◽  
The Impact

AbstractUltrasound-assisted matrix solid phase dispersive extraction was applied for the selective isolation and clean-up of three amphenicol antibiotics, chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FFC) from shrimp. The target antibiotics were separated on a LiChroCART-LiChrospher® 100 RP-18 (5 μm, 250 × 4 mm) analytical column in less than 9 min, with isocratic elution using a mixture of 70% ammonium acetate (0.05 M) and 30% acetonitrile (v/v). Matrix Solid Phase Dispersion protocol was optimized in terms of extraction sorbent and elution solvent. Two polymer based (Oasis and Nexus) sorbents and one silica based (Lichrolut C18) were compared and different elution solvents such as methanol, acetone, acetonitrile and isopropanol were evaluated based on the achieved recovery rates as well as on the cleanup efficiency. The extraction procedure was performed with and without sonication to evaluate the impact of ultrasounds. TAP and FFC were monitored at 234 nm and CAP at 280 nm by a photodiode array detector. The method was validated according to the European Union Decision 2002/657/EC in terms of linearity, selectivity, stability, accuracy, precision and sensitivity. Detection capability values (CCb) were 64.6 μg/kg for TAP and 1046.8 μg/kg for FFC and 63.8 μg/ kg for CAP.

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