Morphology of the pancreatic endocrine A and B cells in normal‐ and epinephrine‐treatedTriturus carnifex

2004 ◽  
Vol 71 (sup2) ◽  
pp. 85-88
Author(s):  
Silvana Buono ◽  
Rosalba Putti
Keyword(s):  
B Cells ◽  
A And B Cells

scholarly journals Characterization of voltage-dependent sodium and calcium channels in mouse pancreatic A- and B-cells

2006 ◽  
Vol 572 (3) ◽  
pp. 691-706 ◽  
Author(s):  
Sheila Vignali ◽  
Veronika Leiss ◽  
Rosi Karl ◽  
Franz Hofmann ◽  
Andrea Welling
Keyword(s):  
B Cells ◽  
Calcium Channels ◽  
Voltage Dependent ◽  
A And B Cells

scholarly journals CYTOCHEMICAL LOCALIZATION OF ADENYL CYCLASE ACTIVITY IN RAT ISLETS OF LANGERHANS

1972 ◽  
Vol 20 (11) ◽  
pp. 873-879 ◽  
Author(s):  
S. L. HOWELL ◽  
MARGARET WHITFIELD
Keyword(s):  
B Cells ◽  
Islets Of Langerhans ◽  
Adenosine Triphosphate ◽  
Adenyl Cyclase ◽  
Cyclase Activity ◽  
Cytochemical Localization ◽  
Adenyl Cyclase Activity ◽  
Rat Islets ◽  
Rat Islets Of Langerhans ◽  
A And B Cells

A cytochemical method has been used to investigate the localization of adenyl cyclase activity in A and B cells of isolated rat islets of Langerhans. Adenosine triphosphate was initially utilized as substrate, the pyrophosphate liberated being precipitated by lead ions at its site of production. The specificity of the method was increased by the use of adenylyl-imidodiphosphate as an alternative substrate; this adenosine triphosphate analogue was not hydrolyzed by adenosine triphosphatase but provided an effective substrate for adenyl cyclase. Adenyl cyclase activity, which was found to retain its glucagon and fluoride sensitivity in glutaraldehyde-fixed tissue, was found exclusively and almost uniformly in the plasma membranes of A and B cells. Storage granule membrane, incorporated into the plasma membrane during secretion of the granule content by exocytosis, appeared to be devoid of adenyl cyclase activity.

INFLUENCE OF THE ISLET A AND B CELLS ON THE EXOCRINE PANCREATIC TISSUE IN THE DUCK

1962 ◽  
Vol 48 (1-2) ◽  
pp. 137-141 ◽  
Author(s):  
Arne Wallgren ◽  
Bo Hellman
Keyword(s):  
B Cells ◽  
Pancreatic Tissue ◽  
A And B Cells

Neurohumoral regulation of the pancreatic islet A and B cells

Metabolism ◽  
1976 ◽  
Vol 25 (11) ◽  
pp. 1453-1456 ◽  
Author(s):  
Daniel Porte ◽  
Phillip H. Smith ◽  
John W. Ensinck
Keyword(s):  
B Cells ◽  
Pancreatic Islet ◽  
Neurohumoral Regulation ◽  
A And B Cells

scholarly journals Morphological and Functional Discrepancies in Endocrine Pancreas After Partial Hepatectomy in Dogs

HPB Surgery ◽  
1991 ◽  
Vol 3 (2) ◽  
pp. 103-116 ◽  
Author(s):  
Tetsuya Hirano ◽  
Tadao Manabe ◽  
Takayoshi Tobe
Keyword(s):  
B Cells ◽  
Partial Hepatectomy ◽  
Endocrine Pancreas ◽  
Point Of View ◽  
Remnant Liver ◽  
Glucose Load ◽  
Pancreatic Glucagon ◽  
Islet Size ◽  
Control Sham ◽  
A And B Cells

To clarify the changes in pancreatic hormones and their role in the regeneration of the liver after partial hepatectomy, we measured the portal levels of insulin and pancreatic glucagon and their responses to a glucose load after about 40% hepatectomy in dogs. The changes in the A and B cells of the islets of Langerhans were examined histologically. In the early stages after hepatectomy portal insulin levels decreased significantly, and the response of portal insulin to a glucose load was lower than in the control sham-operated dogs. Both islet size and the number of B cells increased significantly after hepatectomy. Portal pancreatic glucagon levels increased significantly after hepatectomy, and the response of pancreatic glucagon to a glucose load was not suppressed. The number of A cells also increased significantly.Thus, there were differencies between insulin and pancreatic glucagon in their morphological and functional effects after hepatectomy. Although this difference is not clearly understood, there is a possibility that insulin consumption is accelerated in the remnant liver after hepatectomy. Insulin and pancreatic glucagon appear to play important but different roles in the regenerating liver from the morphological point of view.

scholarly journals Sensitivity of rat pancreatic A and B cells to somatostatin

Diabetologia ◽  
1989 ◽  
Vol 32 (3) ◽  
pp. 207-212 ◽  
Author(s):  
F. C. Schuit ◽  
M. -P. Derde ◽  
D. G. Pipeleers
Keyword(s):  
B Cells ◽  
A And B Cells

scholarly journals AN APPROACH TO THE QUANTITATIVE HISTOCHEMISTRY OF THE A-CELLS IN THE PANCREATIC ISLETS

1966 ◽  
Vol 14 (1) ◽  
pp. 49-52 ◽  
Author(s):  
SVEN E. BROLIN ◽  
ARNE OHLSSON ◽  
ERIK BORGLUND
Keyword(s):  
B Cells ◽  
Islet Cells ◽  
Lactic Dehydrogenase ◽  
Glutamic Oxaloacetic Transaminase ◽  
Malic Dehydrogenase ◽  
Quantitative Histochemistry ◽  
A Cells ◽  
Fresh Frozen ◽  
Darkfield Microscopy ◽  
A And B Cells

Our present knowledge of the quantitative histochemistry of the pancreas is mainly related to the enzymatic properties of the exocrine parenchyma and the B-cells. Appropriate analyses of A-cells require their isolations, which can be accomplished in animals with different islet cells located in separate groups. In fresh frozen sections of the pancreas from ducks groups of A- and B-cells were recognized by darkfield microscopy combined with ordinary microscopic examination after fixation and staining. Each adjacent section was lyophilized and separate samples of acini, A-and B-cells were dissected by free hand. Using quantitative microchemical techniques malic dehydrogenase, lactic dehydrogenase and glutamic-oxaloacetic transaminase activities were measured. The enzymatic distribution found between samples of B-cells and acini was in accordance with earlier findings in mammals, as B-cells showed higher activities except for lactic dehydrogenase. The A-cells differed from the B-cells by lower activities of malic dehydrogenase and glutamic-oxaloacetic transaminase and from the acini by lower activities of lactic dehydrogenase.

scholarly journals Differences in glucose handling by pancreatic A- and B-cells.

1984 ◽  
Vol 259 (2) ◽  
pp. 1196-1200 ◽  
Author(s):  
F K Gorus ◽  
W J Malaisse ◽  
D G Pipeleers
Keyword(s):  
B Cells ◽  
A And B Cells

Cryo-Ultramicrotomy of Islets of Langerhans

1974 ◽  
Vol 15 (3) ◽  
pp. 591-603
Author(s):  
S. L. HOWELL ◽  
MARGARET TYHURST
Keyword(s):  
Polyethylene Glycol ◽  
B Cells ◽  
Islets Of Langerhans ◽  
Structural Features ◽  
Silicotungstic Acid ◽  
Frozen Sections ◽  
Glutaraldehyde Fixation ◽  
Ultrathin Frozen Sections ◽  
Direct Immersion ◽  
A And B Cells

A procedure is described for the preparation of ultrathin frozen sections of glutaraldehyde-fixed or unfixed islets of Langerhans by cryo-ultramicrotomy. Freezing of the tissue was accomplished by direct immersion of isolated islets in liquid nitrogen. Sectioning was performed at a specimen temperature of -80 °C and a knife temperature of -40 °C, the ribbon of sections being collected on a trough containing 60 % dimethyl sulphoxide. Staining was accomplished with 4 % silicotungstic acid and sections were protected from drying artifacts by rinsing with 0.5% polyethylene glycol. Even in tissue not subjected to prior glutaraldehyde fixation, most of the structural features of A and B cells were well preserved in frozen sections, which were obtained in a number and quality which should render them suitable for ultrastructural, cytochemical or radioautographic studies.

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