Plant species delimitation: A comparison of morphological and molecular markers

2009 ◽  
Vol 143 (3) ◽  
pp. 528-542 ◽  
Author(s):  
J. Duminil ◽  
M. Di Michele
Keyword(s):  
Molecular Markers ◽  
Plant Species ◽  
Species Delimitation

scholarly journals Species delimitation and phylogeny of a New Zealand plant species radiation

2009 ◽  
Vol 9 (1) ◽  
pp. 111 ◽  
Author(s):  
Heidi M Meudt ◽  
Peter J Lockhart ◽  
David Bryant
Keyword(s):  
New Zealand ◽  
Plant Species ◽  
Species Delimitation ◽  
Species Radiation

scholarly journals Characterization of rbcL and trnL Plastid DNA Sequences of Vuralia turcica (Fabaceae; Papilionoideae)

2019 ◽  
Vol 7 (6) ◽  
pp. 908
Author(s):  
Dilek Tekdal
Keyword(s):  
Molecular Markers ◽  
Phylogenetic Tree ◽  
Plant Species ◽  
Dna Sequences ◽  
Its Region ◽  
Plastid Dna ◽  
Accession Number ◽  
Molecular Information

Vuralia turcica is endemic to Turkey and currently endangered. Little molecular information is available for this plant. Previous characterization and classification of V. turcica have been based on the DNA sequences of the ITS region. Molecular markers are essential for studying of genotyping and biogeography, but any of each marker is not enough to characterize a plant species in its use alone. In this study, the chloroplast rbcL and trnL regions were amplified in V. turcica using the primers that have been published in the previous studies. Successfully amplified DNA fragments were extracted and commercially sequenced. The partial rbcL and trnL sequences were submitted to the NCBI database (accession number KX164510, KX164511, respectively). Amplified both DNA of two regions of rbcL and trnL were used to construct a phylogenetic tree.

scholarly journals Molecular fingerprinting of lal teer chili variety “morich super f1”

2019 ◽  
Author(s):  
Hossain Sohrawardy ◽  
Salina Sultana
Keyword(s):  
Molecular Markers ◽  
Dna Fingerprinting ◽  
Plant Species ◽  
Polymorphic Band ◽  
Molecular Fingerprinting ◽  
Genetic Purity ◽  
Seed Industry

Abstract Background : Molecular markers do not influence the environment and age of the plant species. DNA fingerprinting is a useful tool for varietal protection for the seed industry. Molecular fingerprinting helps to maintain the genetic purity of parent lines and hybrids. Result: Primer PP 24 and PP 53 showed polymorphic band to Morich Super F 1 and its parents. Conclusion: Morich Super hybridity test conducts at the Lal Teer Biotech Laboratory using these primers.

scholarly journals Termite Taxonomy, Challenges and Prospects: West Africa, A Case Example

Insects ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 32 ◽  
Author(s):  
Judith Korb ◽  
Boris Kasseney ◽  
Yvonne Cakpo ◽  
Robin Casalla Daza ◽  
Jean Gbenyedji ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Species Delimitation ◽  
Morphological Traits ◽  
Global Scale ◽  
West African ◽  
Species Level ◽  
Ecological Research ◽  
Species Specific ◽  
Structured Approach

Termites are important ecosystem engineers. Yet they are often difficult to identify due to the lack of reliable species-specific morphological traits for many species, which hampers ecological research. Recently, termitologists working with West African termites (West African Termite Taxonomy Initiative) convened for a workshop with the aim of beginning to address this problem. Repeated determination of the same termite samples by the most renowned taxonomists for West African termites identified the huge scale of the problem, as less than 10% of all species could be unambiguously determined to the species level. Intensive discussions and comparisons increased the identification success to around 25% at the end of the workshop. Yet many groups remained problematic and molecular markers and barcoding techniques combined with species delimitation approaches will be needed to help resolve these existing taxonomic problems. Based on the outcome of this workshop, we propose concerted initiatives to address termite taxonomy on a global scale. We are convinced that dedicated workshops on regional taxonomy that follow a similar structured approach, with repeated determination of the same sample, will help overcome the difficulties that termite taxonomy faces. This initiative can also serve as a blueprint for other taxonomical groups that are difficult to identify.

scholarly journals Linkage analysis in a full-sib family of an outbreeding plant species: overview and consequences for applications

1997 ◽  
Vol 70 (3) ◽  
pp. 237-250 ◽  
Author(s):  
C. MALIEPAARD ◽  
J. JANSEN ◽  
J. W. VAN OOIJEN
Keyword(s):  
Molecular Markers ◽  
Maximum Likelihood ◽  
Linkage Analysis ◽  
Plant Species ◽  
Recombination Frequency ◽  
Maximum Likelihood Estimators ◽  
Lod Score ◽  
Linkage Phase ◽  
Map Construction ◽  
Sib Families

Linkage analysis and map construction using molecular markers is far more complicated in full-sib families of outbreeding plant species than in progenies derived from homozygous parents. Markers may vary in the number of segregating alleles. One or both parents may be heterozygous, markers may be dominant or codominant and usually the linkage phases of marker pairs are unknown. Because of these differences, marker pairs provide different amounts of information for the estimation of recombination frequencies and the linkage phases of the markers in the two parents, and usually these have to be estimated simultaneously. In this paper we present a complete overview of all possible configurations of marker pairs segregating in full-sib families. Maximum likelihood estimators for the recombination frequency and LOD score formulas are presented for all cases. Statistical properties of the estimators are studied analytically and by simulation. Specific problems of dominant markers, in particular with respect to the probability of detecting linkage, the probability of obtaining zero estimates, and the ability to distinguish linkage phase combinations, and consequences for mapping studies in outbred progenies are discussed.

scholarly journals Molecular markers for plant species identification and phylogenetics

2011 ◽  
Vol 9 (1) ◽  
pp. 32-43 ◽  
Author(s):  
Tatyana V Matveeva ◽  
Olga A Pavlova ◽  
Denis I Bogomaz ◽  
Andrey E Demkovich ◽  
Ludmila A Lutova
Keyword(s):  
Molecular Markers ◽  
Species Identification ◽  
Plant Species ◽  
Nuclear Dna ◽  
Sequencing Data ◽  
Phylogenetic Studies ◽  
Plant Species Identification

 In this review we summarized the information on application of molecular markers for plant species identification and phylogenetics: positive sides and limitations of main markers, representing sequencing data of taxonomically important chloroplast and nuclear DNA regions. Markers, based on polymorphism of PCR and restriction products, are also discussed as accessorial markers in phylogenetic studies. 

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