Repair process and enzymatic activity associated with induction of shell regeneration in the invasive speciesPomacea canaliculata

2016 ◽  
Vol 36 (3) ◽  
pp. 207-212 ◽  
Author(s):  
Song Yang ◽  
Lin-kang Ni ◽  
Liu-lan Zhao ◽  
Jin-wei Yang ◽  
Qiao Liu ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Repair Process ◽  
Shell Regeneration

PEMFs modulate the enzymatic activity during the bone repair process

Bone ◽  
1996 ◽  
Vol 19 (3) ◽  
pp. 133
Author(s):  
V. Canè ◽  
D. Zaffe ◽  
F. Cavani ◽  
P. Botti ◽  
S. Soana
Keyword(s):  
Enzymatic Activity ◽  
Bone Repair ◽  
Repair Process

scholarly journals Indicators of trypsin-like activity of mixed saliva after installing the endosseous implants in the presence of gastric ulcer and duodenal ulcer

2019 ◽  
pp. 13-22
Author(s):  
Mariya Kuznetsova ◽  
Anna Nikonova ◽  
Ivan Kuznetsov ◽  
Mariya Sevbitova ◽  
Mariya Timoshina
Keyword(s):  
Enzymatic Activity ◽  
Negative Impact ◽  
Repair Process ◽  
Peptic Ulcers ◽  
Duodenal Ulcers ◽  
Ulcer Disease ◽  
Implant Placement ◽  
Mixed Saliva ◽  
Orthopedic Patients

The trypsin-like activity of mixed saliva in orthopedic patients after implant placement was monitored. Proved negative impact on the processes of repair of background somatic pathology (peptic ulcers and 12 duodenal ulcers), justify the application of immunomodulator "Erbisol" as a drug that speeds up the repair processes during implantation. At present, the relevant issues are those related to the development of inflammatory complications at the stage of rehabilitation of patients, especially in the presence of somatic pathology and the study of the tissue complex of the implantation zone. Peptic ulcer disease occupies one of the main places in the structure of lesions of the digestive system. The close interdependence between the pathology of internal organs and the oral cavity is confirmed by numerous observations and studies.Implant placement is accompanied by changes in the enzymatic activity of mixed saliva. The presence of somatic pathology (ulcerative disease of the stomach and 12 duodenal ulcers) often leads to more long-term violations of the enzymatic activity of saliva. The use of the drug "Erbisol" in orthopedic dentistry accelerates the repair process, as evidenced by the earlier periods of normalization of the enzymatic activity of mixed saliva, after the installation of implants.Regular and timely monitoring of the condition of the periarticular tissues, as well as objective diagnosis of early inflammatory complications, are necessary to ensure the reliability and long-term functioning of prosthetic structures installed on implants in the oral cavity.It can be concluded that the purpose of our study was to study the trypsin-like activity (TPA) of mixed saliva after implant placement on the background of somatic pathology.

scholarly journals INDICATORS OF TRYPSIN-LIKE ACTIVITY OF MIXED SALIVAAFTER INSTALLING THE ENDOSSEOUS IMPLANTS IN THE PRESENCE OF GASTRIC ULCER AND DUODENAL ULCER

2019 ◽  
Vol 27 (27) ◽  
pp. 13-22
Author(s):  
Mariya KUZNETSOVA ◽  
Anna NIKONOVA ◽  
Ivan KUZNETSOV ◽  
Mariya SEVBITOVA ◽  
Mariya TIMOSHINA
Keyword(s):  
Enzymatic Activity ◽  
Negative Impact ◽  
Repair Process ◽  
Peptic Ulcers ◽  
Duodenal Ulcers ◽  
Ulcer Disease ◽  
Implant Placement ◽  
Mixed Saliva ◽  
Orthopedic Patients

The trypsin-like activity of mixed saliva in orthopedic patients after implant placement was monitored. Proved negative impact on the processes of repair of background somatic pathology (peptic ulcers and 12 duodenal ulcers), justify the application of immunomodulator "Erbisol" as a drug that speeds up the repair processes during implantation. At present, the relevant issues are those related to the development of inflammatory complications at the stage of rehabilitation of patients, especially in the presence of somatic pathology and the study of the tissue complex of the implantation zone. Peptic ulcer disease occupies one of the main places in the structure of lesions of the digestive system. The close interdependence between the pathology of internal organs and the oral cavity is confirmed by numerous observations and studies.Implant placement is accompanied by changes in the enzymatic activity of mixed saliva. The presence of somatic pathology (ulcerative disease of the stomach and 12 duodenal ulcers) often leads to more long-term violations of the enzymatic activity of saliva. The use of the drug "Erbisol" in orthopedic dentistry accelerates the repair process, as evidenced by the earlier periods of normalization of the enzymatic activity of mixed saliva, after the installation of implants.Regular and timely monitoring of the condition of the periarticular tissues, as well as objective diagnosis of early inflammatory complications, are necessary to ensure the reliability and long-term functioning of prosthetic structures installed on implants in the oral cavity.It can be concluded that the purpose of our study was to study the trypsin-like activity (TPA) of mixed saliva after implant placement on the background of somatic pathology.

A Method for the Colorimetric Determination of β-Glucuronidase in Urine, Serum, Tissue; Assay of Enzymatic Activity in Health and Disease

1959 ◽  
Vol 36 (2) ◽  
pp. 193-201 ◽  
Author(s):  
Julius A. Goldbarg ◽  
Esteban P. Pineda ◽  
Benjamin M. Banks ◽  
Alexander M. Rutenburg
Keyword(s):  
Enzymatic Activity ◽  
Colorimetric Determination ◽  
Health And Disease ◽  
Urine Serum

Isolation of Antithrombin III from Normal and α1-Antitrypsin-Deficient Human Plasma

1977 ◽  
Vol 38 (02) ◽  
pp. 0475-0485 ◽  
Author(s):  
Anna D. Borsodi ◽  
Ralph A. Bradshaw
Keyword(s):  
Enzymatic Activity ◽  
Isoelectric Focusing ◽  
Antithrombin Iii ◽  
Factor Xa ◽  
Antithrombin Activity ◽  
Bovine Trypsin ◽  
Normal Plasma ◽  
Antitrypsin Deficiency ◽  
Simplified Procedure ◽  
Stimulation Of

SummaryThe plasma of individuals, hetero- or homozygous for α1-antitrypsin deficiency, contains greatly decreased amounts of antithrombin activity as assayed against factor Xa. However, heparin stimulation of the residual antithrombin activity is observed, which is comparable to that of normal plasma. Antithrombins isolated from both normal and α1-antitrypsin deficient plasma by a simplified procedure are indistinguishable in both properties and yields. The microheterogeneity observed on isoelectric focusing of both preparations can be eliminated by treatment with neuraminidase. Neither purified human antithrombin nor α1-antitrypsin, when assayed against bovine trypsin, is stimulated by heparin. These results clearly establish the unique natures of antithrombin and α1-antitrypsin and show that about 75% of the antithrombin activity measured in normal plasma is due to α1-antitrypsin. Estimates of anti thrombin III activity in normal plasma by assays dependent on enzymatic activity can probably be obtained only in the presence of heparin.

Mechanism of Inhibitory Action on Platelet Activation of a Phospholipase A2 Isolated from Lachesis muta (Bushmaster) Snake Venom

1997 ◽  
Vol 78 (05) ◽  
pp. 1372-1380 ◽  
Author(s):  
André L Fuly ◽  
Olga L T Machado ◽  
Elias W Alves ◽  
Célia R Carlinis
Keyword(s):  
Platelet Aggregation ◽  
Enzymatic Activity ◽  
Phospholipase A2 ◽  
Inhibitory Activity ◽  
Thermal Inactivation ◽  
Anticoagulant Activity ◽  
Gel Filtration ◽  
Apparent Molecular Weight ◽  
Crude Venom ◽  
Lachesis Muta

SummaryCrude venom from Lachesis muta exhibited procoagulant, proteolytic and phospholipase A2 activities. A phospholipase A2, denoted LM-PLA2 was purified from L. muta venom to homogeneity, through a combination of chromatographic steps involving gel-filtration on Sephacryl S-200 HR and reverse phase chromatography on a C2/C18 column. LM-PLA2 presented a single polypeptide chain with an isoelectric point at pH 4.7 and apparent molecular weight of 17 kDa. Partial aminoacid sequence indicated a high degree of homology for LM-PLA2 with other PLA2 from different sources.LM-PLA2 displayed a potent enzymatic activity as measured by indirect hemolysis of red blood cells but it was neither lethal when injected i.p. into mice nor did it present anticoagulant activity. Furthermore, LM-PLA2 displayed a moderate inhibitory activity on the aggregation of rabbit platelets induced by low levels of ADP, thrombin and arachidonate. In contrast, platelet aggregation induced by high doses of collagen was strongly inhibited by LM-PLA2 as well as ATP-release. Treatment of the protein with p-bromophenacyl bromide or 2-mercapto-ethanol, as well as thermal inactivation studies, suggested that the platelet inhibitory effect of LM-PLA2 is dependent on its enzymatic activity. Thus, the platelet inhibitory activity of LM-PLA2 was shown to be dependent on the hydrolysis of plasma phospholipids and/or lipoproteins, most probably those rich in phosphatidylcholine. Surprisingly, lyso-phosphatidylcholine released by LM-PLA2 from plasma was shown to preferentially inhibited collagen-induced platelet aggregation, in contrast to other PLA2s, whose plasma hydrolytic products indistinctly affect platelet’s response to several agonists.

Degradation of 2,4-dichlorophenol by melamine amine celluloseimmobilized lacasses

TAPPI Journal ◽  
2017 ◽  
Vol 16 (10) ◽  
pp. 559-564 ◽  
Author(s):  
ZHEN WANG ◽  
PINGPING BIN ◽  
YING LIU ◽  
YU LIU ◽  
GUIHUA YANG ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Removal Efficiency ◽  
Immobilized Laccase ◽  
Dialdehyde Cellulose

Epoxidized dialdehyde cellulose (EDC) was prepared and grafted with melamine to obtain melamine grafted epoxidized dialdehyde cellulose (EDC-melamine); the products were characterized by various methods and were used as carriers to immobilize laccase. Results show EDC-melamine can immobilize laccase effectively and have higher enzymatic activity compared with EDC. Furthermore, the enzymatic activity of EDC-melamine was found to be as high as 865 U•mg-1, compared with 140U•mg-1 for EDC. The removal efficiency of 2,4-dichlorophenol (2,4-DCP) for EDC-melamine immobilized laccase was about 71.5% at 40°C for 4 h at 10.0 mg•L-1 and dosage of laccase = 0.2 g/L. The removal efficiency can remain greater than 63%, even after six cycles.

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