PEMFs modulate the enzymatic activity during the bone repair process

Bone ◽  
1996 ◽  
Vol 19 (3) ◽  
pp. 133
Author(s):  
V. Canè ◽  
D. Zaffe ◽  
F. Cavani ◽  
P. Botti ◽  
S. Soana
Keyword(s):  
Enzymatic Activity ◽  
Bone Repair ◽  
Repair Process

Enhancement of Bone Healing by Local Administration of Carbon Nanotubes Functionalized with Sodium Hyaluronate in Rat Tibiae

2017 ◽  
Vol 204 (3-4) ◽  
pp. 137-149 ◽  
Author(s):  
Vanessa B. Andrade ◽  
Marcos A. Sá ◽  
Renato M. Mendes ◽  
Paulo A. Martins-Júnior ◽  
Gerluza A.B. Silva ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Bone Repair ◽  
Bone Matrix ◽  
Sodium Hyaluronate ◽  
Repair Process ◽  
Bone Defects ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Bone Trabeculae ◽  
Endothelial Growth Factor

It has been reported that carbon nanotubes (CNTs) serve as nucleation sites for the deposition of bone matrix and cell proliferation. Here, we evaluated the effects of multi-walled CNTs (MWCNTs) on bone repair of rat tibiae. Furthermore, because sodium hyaluronate (HY) accelerates bone restoration, we associated CNTs with HY (HY-MWCNTs) in an attempt to boost bone repair. The bone defect was created by a 1.6-mm-diameter drill. After 7 and 14 days, tibiae were processed for histological and morphometric analyses. Immunohistochemistry was used to evaluate the expression of vascular endothelial growth factor (VEGF) in bone defects. Expression of osteocalcin (OCN), bone morphogenetic protein-2 (BMP-2), and collagen I (Col I) was assessed by real-time PCR. Histomorphometric analysis showed a similar increase in the percentage of bone trabeculae in tibia bone defects treated with HY and HY-MWCNTs, and both groups presented more organized and thicker bone trabeculae than nontreated defects. Tibiae treated with MWCNTs or HY- MWCNTs showed a higher expression of VEGF. Treatment with MWCNTs or HY-MWCNTs increased the expression of molecules involved in the bone repair process, such as OCN and BMP-2. Also, HY- and MWCNT-treated tibiae had an increased expression of Col I. Thus, it is tempting to conclude that CNTs associated or not with other materials such as HY emerged as a promising biomaterial for bone tissue engineering.

EFFECTS OF Justicia gendarussa ETHANOLIC EXTRACT ON OSTEOBLASTIC ACTIVITY OF MC3T3-E1 CELL

2015 ◽  
Vol 77 (3) ◽  
Author(s):  
Kavita Supparmaniam ◽  
Siti Pauliena Mohd Bohari
Keyword(s):  
Bone Repair ◽  
Bone Fractures ◽  
Bone Mineralization ◽  
Ethanolic Extract ◽  
Repair Process ◽  
New Bone Formation ◽  
Osteoblast Cells ◽  
Osteoblastic Activity ◽  
Alp Activity ◽  
Ic50 Value

Justicia gendarussa (Acanthaceae) or commonly known as Gendarussa has traditionally been used to treat bone fractures. Bone fracture is a clinical condition that need bone repair and new bone formation. To date, the mechanism of Justicia gendarussa acting in enhancing the bone mineralization has not been proven scientifically. The present study aimed to investigate the cytotoxicity and alkaline phosphatase (ALP) activity on osteoblast cells when treated with Justicia gendarussa ethanolic leaves extract. MTT and ALP assays were performed on osteoblast cells after being treated with different concentrations of the extract. For cell viability, the result showed that IC50 value of the osteoblast cells was 89.1μg/ml. While, ALP assay is used as a biochemical marker for early detection of osteoblast mineralization. The highest amount of ALP activity was at the 37.5 μg/ml when compared to the control. From this study, it shows that Justicia gendarussa has potential in enhancing bone mineralization during the bone repair process.  

scholarly journals Fibrin Sealant Derived from Human Plasma as a Scaffold for Bone Grafts Associated with Photobiomodulation Therapy

2019 ◽  
Vol 20 (7) ◽  
pp. 1761 ◽  
Author(s):  
Karina Torres Pomini ◽  
Daniela Vieira Buchaim ◽  
Jesus Carlos Andreo ◽  
Marcelie Priscila de Oliveira Rosso ◽  
Bruna Botteon Della Coletta ◽  
...  
Keyword(s):  
Bone Formation ◽  
Support System ◽  
Fibrin Sealant ◽  
Bone Repair ◽  
Blood Clot ◽  
Repair Process ◽  
Volume Density ◽  
Photobiomodulation Therapy ◽  
Positive Effect ◽  
Therapy Protocol

Fibrin sealants derived from human blood can be used in tissue engineering to assist in the repair of bone defects. The objective of this study was to evaluate the support system formed by a xenograft fibrin sealant associated with photobiomodulation therapy of critical defects in rat calvaria. Thirty-six rats were divided into four groups: BC (n = 8), defect filled with blood clot; FSB (n = 10), filled with fibrin sealant and xenograft; BCPBMT (n = 8), blood clot and photobiomodulation; FSBPBMT (n = 10), fibrin sealant, xenograft, and photobiomodulation. The animals were killed after 14 and 42 days. In the histological and microtomographic analysis, new bone formation was observed in all groups, limited to the defect margins, and without complete wound closure. In the FSB group, bone formation increased between periods (4.3 ± 0.46 to 6.01 ± 0.32), yet with lower volume density when compared to the FSBPBMT (5.6 ± 0.45 to 10.64 ± 0.97) group. It was concluded that the support system formed by the xenograft fibrin sealant associated with the photobiomodulation therapy protocol had a positive effect on the bone repair process.

Systemic Mesenchymal Stem Cell Delivery and Axial Mechanical Stimulation Accelerate Fracture Healing

2008 ◽  
Author(s):  
Aaron S. Weaver ◽  
Yu-Ping Su ◽  
Dana L. Begun ◽  
Ralph T. Zade ◽  
Andrea I. Alford ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Bone Repair ◽  
Local Environment ◽  
Cell Types ◽  
Repair Process ◽  
Fracture Site ◽  
Fracture Repair ◽  
Stem Cell Delivery ◽  
Numerous Cell ◽  
Local Cell

Fracture healing is a complex process involving numerous cell types, whose actions are regulated by many factors in their local environment. Mechanical factors are known to exert a strong influence on the actions of these cells and the progression of the repair process. While prior studies have investigated the effect of physical forces on cell differentiation, biofactor expression, and mechanical competence of repair, the mechanosensory and response mechanisms are poorly understood. This study was designed to explore the influence of a controlled mechanical environment on temporal aspects of the bone repair process. Specifically, this study examines how the timing of an applied strain influences local cell behavior during fracture repair, and how this load affects the migration of systemically introduced mesenchymal stem cells (MSCs) to the fracture site.

Bone Repair with Compressive Sensing Based on MRI

2013 ◽  
Vol 380-384 ◽  
pp. 4019-4022
Author(s):  
Feng Zhou ◽  
Yi Jian Pei ◽  
Hao Wu ◽  
Zhi Jun Chen
Keyword(s):  
Bone Repair ◽  
Sparse Matrix ◽  
Wavelet Coefficient ◽  
Medical Science ◽  
Repair Process ◽  
Treatment Programs ◽  
Pathological Features ◽  
High Quality ◽  
Compressed Images ◽  
Visualization Techniques

With the development of society, much technology has been invented, many problems have been conquered. But it is still very hard for medical science. For example, physicians usually obtain the lesion information by means of medical imaging equipment and computer visualization techniques, to judge the symptoms, and develop appropriate treatment programs. However, it is hard for Magnetic Resonance Imaging (MRI) equipment to get high-quality image of the lesion, and then extract the pathological features of high quality. This paper will introduce a method to overcome this tough stuff. This paper attempts to adopt compressed sensing technology for image processing in the bone repair process. Lesion images are first acquired via MRI, then with the wavelet transform, to get the sparse matrix by the wavelet coefficient sparse representation. The method can obtain compressed images, and extract the corresponding pathological features without reducing the image quality.

scholarly journals Repair of Bone Defects with Chitosan-Collagen Biomembrane and Scaffold Containing Calcium Aluminate Cement

2017 ◽  
Vol 28 (3) ◽  
pp. 287-295 ◽  
Author(s):  
Paola Castro de Moraes ◽  
Isabela Cristina de Souza Marques ◽  
Fernanda Gonçalves Basso ◽  
Hebert Luis Rossetto ◽  
Fernanda de Carvalho Panzeri Pires-de-Souza ◽  
...  
Keyword(s):  
Calcium Aluminate ◽  
Bone Repair ◽  
Repair Process ◽  
Bone Defects ◽  
Calcium Aluminate Cement ◽  
Histopathological Analysis ◽  
Body Type ◽  
Significant Difference ◽  
Autologous Bone ◽  
Aluminate Cement

Abstract Innovative biomaterials can provide a promising new direction for the treatment of bone defects, stimulating a proper repair process, with no damage to adjacent tissues. The purpose of this in vivo study was to evaluate the biocompatibility and the osteoinductive capacity of chitosan-collagen biomembrane and scaffold containing calcium aluminate cement. Eighteen New Zealand white rabbits (Oryctolagus cuniculus) were distributed according to the experimental times of analysis (7, 15 and 30 days). Four bone defects were created in the rabbits calvaria, which were individually filled with the biomembrane, scaffold, blood clot (negative control) and autologous bone (positive control). Histopathological analysis was performed using optical microscope at 32´, 64´, 125´ and 320´ magnifications. Cell response to inflammation and new bone tissue formation was quantified using a score system. The biomembrane group presented greater inflammatory response at 15 days, with significant difference to autologous bone group (p<0.05). There was no statistically significant difference for foreign body type reaction among groups (p>0.05). Concerning new bone formation, linear closure of the defect area was observed more evidently in the group with autologous bone. The scaffold group presented similar results compared with the autologous bone group at 30 days (p>0.05). Both tested biomaterials presented similar biocompatibility compared with the control groups. In addition, the biomembrane and scaffold presented similar osteoinductive capacity, stimulating bone repair process in the course of the experimental time intervals.

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