Development of a dot blot technique for rapid identi®cation of Botrytis cinerea, the causal organism of grey mould in greenhouse tomatoes

Grey mould, caused by Botrytis cinerea Pers.:Fr. is one of the most important strawberry diseases in Lithuania, like in other countries, where strawberries are grown. The efficiency of different disease management systems were analyzed at the Institute of Horticulture in 2010-2011. The B. cinerea risk probability at various regions of Lithuania was analyzed according to iMETOS ®sm grey mould risk forecasting model. Strawberry grey mould risk forecasting model indicates the risk of infection periods on the basis of the interaction between air temperature and leaf wetness duration. The model calculates how favourable is the period for the risk of infection. In periods where the risk is consistent (more than three days), higher than 60 points, a spray against grey mould should be applied. iMETOS®sm grey mould risk forecasting model gives the opportunity to optimize the usage of fungicides and reduce the number of applications and allows more efficient, ecologically and economically accepted control of strawberries grey mould.

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Improved leaf pruning reduces development of stem cankers caused by grey mould in greenhouse tomatoes

Agronomy for Sustainable Development ◽

10.1051/agro/2009030 ◽

2010 ◽

Vol 30 (2) ◽

pp. 465-472 ◽

Cited By ~ 8

Author(s):

V. Decognet ◽

F. Ravetti ◽

C. Martin ◽

P. C. Nicot

Keyword(s):

Grey Mould ◽

Greenhouse Tomatoes ◽

Stem Cankers

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Host‐induced gene silencing of BcTOR in Botrytis cinerea enhances plant resistance to grey mould

Molecular Plant Pathology ◽

10.1111/mpp.12873 ◽

2019 ◽

Vol 20 (12) ◽

pp. 1722-1739 ◽

Cited By ~ 1

Author(s):

Fangjie Xiong ◽

Mei Liu ◽

Fengping Zhuo ◽

Huan Yin ◽

Kexuan Deng ◽

...

Keyword(s):

Gene Silencing ◽

Botrytis Cinerea ◽

Plant Resistance ◽

Grey Mould

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Supression of Botrytis cinerea causing grey mould disease of grape vine (Vitis vinifera) and its pectinolytic activities by a soil bacterium

Microbiological Research ◽

10.1016/s0944-5013(97)80060-2 ◽

1997 ◽

Vol 152 (4) ◽

pp. 413-420 ◽

Cited By ~ 15

Author(s):

Bernard Paul ◽

Irène Girard ◽

Tej Bhatnagar ◽

Philippe Bouchet

Keyword(s):

Vitis Vinifera ◽

Botrytis Cinerea ◽

Grey Mould ◽

Soil Bacterium ◽

Grape Vine

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Comparison Strawberry Field Production and Occurrence of Grey Mould at Strawberry Growing on System with Addition of Apple Tree Woody Chips

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis ◽

10.11118/actaun201765041253 ◽

2017 ◽

Vol 65 (4) ◽

pp. 1253-1264

Author(s):

David Novotný ◽

Jana Brožová ◽

Pavla Růžičková ◽

Josef Sus ◽

Martin Koudela ◽

...

Keyword(s):

Botrytis Cinerea ◽

Apple Tree ◽

Grey Mould ◽

Fruit Weight ◽

Wood Chips ◽

Average Weight ◽

Tree Wood ◽

Significant Difference ◽

And Control ◽

Field Production

In the years 2014 to 2016 the influence of apple tree wood chips and composted apple tree wood chips in which oyster mushroom grew and yielded which were supplemented into soil on strawberry field production and occurrence of grey mould at strawberry (cv. ‘Sonata’) was evaluated. Average weight and number of fruits per plant, average fruit weight, average weight and number of fruits affected by Botrytis cinerea per plant, percentage of weight and number of fruits affected by Botrytis cinerea was calculated. There were recorded differences concerning strawberry yield among the investigated variants of plots, but mainly there were not statistically significant. In 2015 and 2016 the highest average weight of yield and the highest number of fruits was reached at plants cultivated on plots with apple tree wood chips. Average weight of yield on plots with apple tree wood chips was 663.72 g and 822.41 g in 2015 and 2016, respectively. Number of fruits per plant on plots with apple tree wood chips was 67.88 pieces and 65.29 pieces in 2015 and 2016, respectively. Occurrence of grey mould caused by Botrytis cinerea on all variants of plots was similar and small differences was found out only but statistically significant difference was among the years. Grey mould was the most frequently found out in the year 2016. B. cinerea affected 48.27, 56.89 and 55.94 grams of fruits per plant from plots with composted apple tree wood chips, plots with apple tree wood chips and control plots in 2016, respectively.

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Development and standardization of the Dot Blot test for serological diagnosis of bovine leptospirosis

Research Society and Development ◽

10.33448/rsd-v10i6.15091 ◽

2021 ◽

Vol 10 (6) ◽

pp. e22710615091

Author(s):

Higor Oliveira Silva ◽

Mariana Assunção de Souza ◽

Tatiane Cristina Fernandes Tavares ◽

Pollyanna Mafra Soares ◽

Anna Monteiro Correia Lima

Keyword(s):

Membrane Protein ◽

Outer Membrane ◽

Outer Membrane Protein ◽

Predictive Value ◽

Bovine Serum ◽

Low Cost ◽

High Sensitivity ◽

Dot Blot ◽

Blot Technique ◽

Bovine Leptospirosis

Leptospirosis a public health problem and an endemic zoonosis in Brazil, is diagnosed by serological methods. Therefore, low-cost and easy to execute methodologies with good/high sensitivity, such as Dot Blot, are an important diagnostic tool. The aim of this study was to standardize and validation the dot-blot technique for the serological diagnosis of bovine leptospirosis. Several concentrations of antigens applied to nitrocellulose membranes, and different dilutions of conjugated bovine serum were evaluated to develop and standardize the test. The best distinction/contrast between positive and negative samples was observed for 1μL antigen (0.11μg/μL outer membrane protein of the Hardjo serovar (OMPH) and 0.08 μg/μL outer membrane protein of the Wolffi serovar (OMPW)), 1:500 and 1:10000 bovine serum dilution and conjugate, respectively. The Dot Blot test efficiency was 71.87% and kappa index, 0.46 (p<0.0001). The other parameters measured were: sensitivity 91.89%; specificity 59.32%; positive predictive value 58.62%; and, negative predictive value 59.32%. In addition to high sensitivity, other advantages of the Dot Blot technique have been identified, such as practicality, low cost since it does not require sophisticated devices and the fact that the Hardjo and Wolffi OMP also reacted with serovars from other pathogenic serogroups. The results provided positive expectations for the use of Dot Blot as support in the diagnosis of bovine leptospirosis, especially if used as a screening test, stimulating further research for the future development of kits for diagnostic purposes.

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