Genetic Variability of Klebsiella Variicola by RAPD-PCR Technique and Bioremoval of Pb2+ and Cd2+ from Simulated Contaminated Soils

Blood samples were collected from Pantaneiro Horses in five regions of Mato Grosso do Sul and Mato Grosso States. Arabian, Mangalarga Marchador and Thoroughbred were also included to estimate genetic distances and the existing variability among and within these breeds by RAPD-PCR (Random Amplified Polymorphic DNA - Polymerase Chain Reaction) molecular markers. From 146 primers, 13 were chosen for amplification and 44 polymorphic bands were generated. The analysis of molecular variance (AMOVA) indicated that the greatest portion of detected variability was due to differences between individuals within populations (75.47%). Analysis of the genetic variability between pairs of populations presented higher estimates for the five Pantaneiro populations with the Arabian breed, while lowest estimates were presented by pairs formed among the Pantaneiro populations with the Mangalarga Marchador. Highest genic diversity was shown by the Pantaneiro (0.3396), which also showed highest genetic distance with the Arabian and lowest with Mangalarga Marchador breed. UPGMA dendrogram showed distinct differences between naturalized (Pantaneiro and Mangalarga Marchador) and exotic (Arabian and Thoroughbred) breeds. In the dendrogram generated by UPGMA method, the similarity matrix generated by the Jaccard coefficient showed distinction between the naturalised breeds, Pantaneiro and Mangalarga Marchador, and the exotic breeds, Árab and English Thoroughbred. Results suggest that the Pantaneiro presents a higher genetic variability than the other studied breeds and has a close relationship with the Mangalarga Marchador.

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Genetic diversity of captive spotted paca (Agouti paca) from south east Brazil assessed by the RAPD-PCR technique

Revista Brasileira de Zootecnia ◽

10.1590/s1516-35982010000200006 ◽

2010 ◽

Vol 39 (2) ◽

pp. 268-272

Author(s):

Karine Vieira Antunes ◽

Théa Mírian Medeiros Machado ◽

Nicola Vergara Lopes Serão ◽

Simone Eliza Facione Guimarães ◽

Samuel Rezende Paiva

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Population Genetic ◽

Geographical Information ◽

Breeding Sites ◽

Rapd Pcr ◽

Reproductive Male ◽

Males And Females ◽

Structured Groups ◽

Breeding Farms

The genetic diversity was analyzed among spotted paca (Agouti paca) from three commercial flocks located in Brazil. As the genome of this species is unknown, the RAPD-PCR technique was used. Ten primers generated sixty polymorphic bands. The among and within population genetic variability estimated by analysis of molecular variance (AMOVA) was 12.55 and 87.45%, respectively. The shortest Nei distance value was 11.76% among the Carangola (CG) and São Francisco do Glória (SF) populations. This value can be explained by the exchange of reproduction males and females between the two geographically close breeding sites. The analysis of principal components showed well defined and structured groups aggregating animals according their population of origin, with some exceptions. Lower diversity was found in the São Francisco population than in the Carangola and Castelo (CS) populations. This result suggested the variability is better conserved in breeding farms with fifty or sixty animals (CG and CS) than in the breeding farm with a dozen animals (SF). The RADP-PCR technique proved to be informative for the quantification of among and within population genetic variability of the spotted paca. The phenogram generated by UPGMA using the NTSYS-PC software from the Nei Distance, grouped CG and SF on a single branch connected to the CS, with 76 and 100% accuracy, respectively, to the bootstrap. This result was not only consistent with the historical and geographical information on flocks, but also shows the need for periodic reproductive male replacement. Future studies should be developed with co-dominant markers and include spotted paca from more distant places.

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Optimization of DNA isolation and PCR parameters for RAPD analysis of Gonyostomum semen (Raphidophyceae)

Botanica Lithuanica ◽

10.2478/v10279-012-0006-6 ◽

2012 ◽

Vol 18 (1) ◽

pp. 40-45

Author(s):

Elena Servienė ◽

Irena Kemežienė ◽

Jūratė Kasperovičienė ◽

Brigita Čapukoitienė ◽

Vida Rančelienė ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Genetic Variability ◽

Genomic Dna ◽

Dna Isolation ◽

Rapd Analysis ◽

Dna Purification ◽

High Quality ◽

Purification Method ◽

Rapd Pcr ◽

Gonyostomum Semen

Abstract Servienė E., Kemežienė I., Kasperovičienė J., čapukoitienė B., Rančelienė V., Koreivienė J., 2012: Optimization of DNA isolation and PCR parameters for RAPD analysis of Gonyostomum semen (Raphidophyceae) [DNR izoliavimas ir PGR parametrų optimizavimas Gonyostomum semen (Raphidophyceae) dumblių RAPD analizei]. - Bot. Lith., 18(1): 40-45. The genomic DNA purification method for Gonyostomum semen algae was optimized by applying different DNA purification techniques and rational modifications. This method allowed to obtain high quality DNA preparations suitable for the phylogenetic analysis and genetic variability investigation of algae. DNA isolated by this method yielded strong and reliable amplification products showing their applicability for RAPD-PCR using random decamer primers. In the present study, the RAPD protocol was optimized for the evaluation of Gonyostomum biodiversity.

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Study of Genetic Variability Among Monopycnidial and Monopycnidiospore Isolates Derived from Single Pycnidia of Stagonospora ssp. and Septoria tritici with the use of RAPD-PCR, MP-PCR and rep-PCR Techniques

Journal of Phytopathology ◽

10.1046/j.1439-0434.1999.00434.x ◽

1999 ◽

Vol 147 (9) ◽

pp. 539-546 ◽

Cited By ~ 3

Author(s):

P. C. Czembor ◽

E. Arseniuk

Keyword(s):

Genetic Variability ◽

Septoria Tritici ◽

Rapd Pcr ◽

Pcr Techniques ◽

Pcr Mp

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Evaluation of the Genetic Variability among a Wild Peganum Harmala L. Populations with RAPD-PCR

MOJ Biology and Medicine ◽

10.15406/mojbm.2017.01.00015 ◽

2017 ◽

Vol 1 (3) ◽

Author(s):

Ibrahim Mohammad Al Rawashdeh

Keyword(s):

Genetic Variability ◽

Peganum Harmala ◽

Rapd Pcr

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Genetic variability estimated with RAPD-PCR markers in two populations of Heterodera glycines Ichinohe, 1952 (Nematoda: Heteroderidae) from Argentina

Nematology ◽

10.1163/156854104323072874 ◽

2004 ◽

Vol 6 (1) ◽

pp. 13-21 ◽

Cited By ~ 3

Author(s):

Paola Lax ◽

Juan Rondan Dueñas ◽

Cristina Gardenal ◽

Marcelo Doucet

Keyword(s):

Genetic Variability ◽

Management Practices ◽

Heterodera Glycines ◽

Control Strategies ◽

Random Amplified Polymorphic Dna ◽

Allele Frequencies ◽

Economic Losses ◽

Genetic Population ◽

Rapd Pcr ◽

Two Populations

Abstract Knowledge of genetic variability within and between phytophagous nematode populations is important for the selection of suitable control strategies. The nematode Heterodera glycines is the most important pathogen of soybean in many producer countries, causing significant economic losses. The levels of variability were analysed in two populations of the nematode from Argentina, using Random Amplified Polymorphic DNA markers in order to evaluate the genetic population structure of this species. DNA was extracted from single females of race 1 and race 3 populations. Five primers were selected and 50 consistent fragments were considered for further analysis. Populations studied showed high levels of genetic diversity. Most of the total variation detected in allele frequencies was attributed to variability among individuals of the same population (83%) rather than inter-population differences (17%). None of the populations demonstrated exclusive bands. However, 68% of fragments showed significant differences in their allele frequencies. This study revealed an important degree of genetic differentiation between both populations, probably as a consequence of limited gene flow between them or because each population was under different management practices at its site of origin. Results herein represent the first analysis of genetic structure in H. glycines populations using RAPD-PCR technique.

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Assessment of genetic variation in wild populations of the redclaw crayfish (Cherax quadricarinatus, von Martens 1868) by means of allozyme and RAPD-PCR markers

Marine and Freshwater Research ◽

10.1071/mf9951217 ◽

1995 ◽

Vol 46 (8) ◽

pp. 1217 ◽

Cited By ~ 30

Author(s):

JM Macaranas ◽

PB Mather ◽

P Hoeben ◽

MF Capra

Keyword(s):

Genetic Variability ◽

Rapd Analysis ◽

Electrophoretic Analysis ◽

Geographic Proximity ◽

Pcr Markers ◽

River Population ◽

Cherax Quadricarinatus ◽

Rapd Pcr ◽

Low Levels ◽

Redclaw Crayfish

The population structure of the redclaw crayfish (Cherax quadricarinatus) was investigated by analyses of allozymes and RAPD (randomly amplified polymorphic DNA) markers. Electrophoretic analysis of 28 enzyme loci in 12 redclaw populations from the Northern Territory (NT) and North Queensland (NQ) revealed generally low estimates of heterozygosity within each population and low estimates of genetic differentiation among populations except for a fixed allelic difference at the carbonic anhydrase (CA*) locus between NT and NQ populations. Low levels of genetic variability among the NQ populations is possibly a reflection of their recent radiation across the Gulf of Carpentaria after its inundation between 18000 and 6000 BP. RAPD analysis of seven redclaw populations could distinguish each river population and also grouped them according to geographic proximity. RAPD analyses also revealed significant genetic variability both within the species and within individual populations, which could be used to improve their culture. On a preliminary scale, RAPD fragments provided a useful tool for differentiating redclaw strains and constitute a marking system that could be used in crayfish genetic improvement programmes.

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