Selection of Crystal Face of Calcium Oxalate Monohydrate Beneath Dppc Monolayers in the Presence of Chondroitin Sulfate A

The influence of urinary macromolecule chondroitin sulfate A (C4S) on Zeta potential, aggregation and sedimentation of calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) nanocrystallites with a diameter of about 50 nm were investigated using nanoparticle size Zeta potential analyzer and transmission electron microscope. C4S could increase the absolute value of Zeta potential on surface of nanoCOM and nanoCOD crystallites by adsorbing on crystal surface, it led an increase of the electrostatic repulsion force between the crystallites, so the aggregation and sedimentation of these crystallites were inhibited, and the stability of the suspension of nanoCOM and nanoCOD was improved. In conclusion, C4S could inhibit the formation of CaOxa stones。

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Chondroitin Sulfate: A Critical Review of Generic and Specific Problems in Its Characterization and Determination—An Exemplar of a Material with an Unknown or Variable Composition (UVCB)

Journal of AOAC International ◽

10.5740/jaoacint.17-0115 ◽

2018 ◽

Vol 101 (1) ◽

pp. 196-202 ◽

Cited By ~ 3

Author(s):

Duncan Thorburn Burns ◽

Michael John Walker ◽

Christopher Mussell

Keyword(s):

Chondroitin Sulfate ◽

Critical Review ◽

Analytical Methods ◽

Cetylpyridinium Chloride ◽

Variable Composition ◽

Reference Samples ◽

Selection Of ◽

Chondroitin Sulfate A

Abstract This review discusses the criteria for the selection of appropriate reference samples of chondroitin sulfate (CS) and the properties and specific problems of analytical methods for CS, namely titration with cetylpyridinium chloride; various separations; and UV-Vis, NMR, MS, and IR spectroscopies. Suggestions are put forward with regard to acceptable protocols for manufactures’ and for official/referee analysts for the analysis of CS in products.

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Adhesion of calcium oxalate monohydrate crystals to renal epithelial cells is inhibited by specific anions

AJP Renal Physiology ◽

10.1152/ajprenal.1995.268.4.f604 ◽

1995 ◽

Vol 268 (4) ◽

pp. F604-F612 ◽

Cited By ~ 36

Author(s):

J. C. Lieske ◽

R. Leonard ◽

F. G. Toback

Keyword(s):

Epithelial Cells ◽

Cell Surface ◽

Calcium Oxalate ◽

Chondroitin Sulfate ◽

Kidney Stones ◽

Calcium Oxalate Monohydrate ◽

Apical Surface ◽

Dependent Manner ◽

Renal Epithelial Cells ◽

Renal Tubular Cells

Adhesion of urinary crystals to the apical surface of renal tubular cells could be a critical step in the formation of kidney stones. The interaction between renal epithelial cells (BSC-1 line) and the most common crystal in kidney stones, calcium oxalate monohydrate (COM), was studied in a tissue culture model system. COM crystals bound to the cell surface within seconds in a concentration-dependent manner to a far greater extent than did brushite, another calcium-containing crystal found in urine. Adhesion of COM crystals to cells was blocked by the polyanion, heparin. Other glycosaminoglycans including chondroitin sulfate A or B, heparan sulfate, and hyaluronic acid, but not chondroitin sulfate C, prevented binding of COM crystals. Two nonsulfated polyanions, polyglutamic acid and polyaspartic acid, also blocked adherence of COM crystals. Three molecules found in urine, nephrocalcin, uropontin, and citrate, each inhibited binding of COM crystals, whereas Tamm-Horsfall glycoprotein (THP) did not. Prior exposure of crystals but not cells to inhibitory molecules blocked adhesion, suggesting that these agents exert their effect at the crystal surface. Inhibition of crystal binding followed a linear Langmuir adsorption isotherm for each inhibitor identified, suggesting that these molecules bind to a single class of sites on the crystal that are important for adhesion to the cell surface. Inhibition of crystal adhesion by heparin was rapidly overcome by the polycation protamine, suggesting that the glycosaminoglycan regulates cell-crystal interactions in a potentially reversible manner.(ABSTRACT TRUNCATED AT 250 WORDS)

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Effects of dipalmitoylphosphatidylcholine monolayers to the crystallization of calcium oxalate monohydrate from the solution containing chondroitin sulfate C

Colloids and Surfaces A Physicochemical and Engineering Aspects ◽

10.1016/j.colsurfa.2004.10.047 ◽

2005 ◽

Vol 257-258 ◽

pp. 47-50 ◽

Cited By ~ 9

Author(s):

Sui-Ping Deng ◽

Jian-Ming Ouyang

Keyword(s):

Calcium Oxalate ◽

Chondroitin Sulfate ◽

Calcium Oxalate Monohydrate

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Adsorption of dextran and dextran sulfate on precipitated calcium oxalate monohydrate

Journal of Colloid and Interface Science ◽

10.1016/s0021-9797(84)80054-5 ◽

1984 ◽

Vol 98 (1) ◽

pp. 373-384 ◽

Cited By ~ 2

Author(s):

V HLADY

Keyword(s):

Calcium Oxalate ◽

Dextran Sulfate ◽

Calcium Oxalate Monohydrate

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Can stone composition be predicted by plain X-ray and/or non-contrast CT? A study validated by X-ray diffraction analysis

African Journal of Urology ◽

10.1186/s12301-020-00080-3 ◽

2020 ◽

Vol 26 (1) ◽

Author(s):

Mohamed Gadelmoula ◽

Ahmed M. Moeen ◽

Ahmed Elderwy ◽

Mohamed S. Abdel-Kader ◽

Ayman Elqady ◽

...

Keyword(s):

Calcium Oxalate ◽

Diffraction Analysis ◽

Great Influence ◽

Calcium Oxalate Monohydrate ◽

Categorical Variables ◽

Stone Analysis ◽

Stone Composition ◽

Cutoff Value ◽

X Ray ◽

Contrast Ct

Abstract Background The stone composition has a great influence on the outcome of its treatment. There are several tests to predict the composition of stones preoperatively and stone analysis postoperatively. Herein, we want to evaluate if the stone composition could be predicted from plain X-ray KUB (PKUB) and/or non-contrast CT (NCCT) validated by in vitro X-ray powder diffraction analysis (XRD). Methods Between April 2014 and March 2016, 100 cases with urinary tract stones were included in the study. The radio-opacity of the stones in PKUB, stone density by NCCT, and after stone extraction, XRD were performed. Statistical analysis for the results was performed using Chi-square and Fisher exact tests for categorical variables and Mann–Whitney U and Kruskal–Wallis H for the nonparametric variables. The receiver operating characteristic curve was constructed to determine the best cutoff value. Results This study included 74 males and 26 females with a median age of 32 years (range 2–70). Regarding the radio-opacity by PKUB, there were 30 stones dense opaque, 44 opaque, 21 faint opaque, and 5 radiolucent. XRD revealed 97 mixed and 3 pure stones. The calcium oxalate monohydrate (COM) stone composition could be predicted in dense opaque stone by PKUB in 75.9% and urate composition in the radiolucent stone by 40%. The cutoff value of HU density by NCCT to the dense opaque stones in the PKUB was > 1020 and for radiolucent stones was < 590. Conclusion Stone radio-opacity by PKUB and its attenuation value by NCCT could successfully predict its calcium oxalate monohydrate, struvite, and urate composition. However, the chemical stone analysis is still required as most stones are mixed.

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