Sampling and Analytical Method Development and Hand Wipe Measurements of Dermal Exposures to Polycyclic Aromatic Hydrocarbons

AbstractThe aim of this study was to develop an analytical method, which separates selected chlorinated polycyclic aromatic hydrocarbons (Cl-PAHs) from fat, and fat-free or vegetable matrices. The method contains extraction-, cleanup-, and quantification steps. Integration of automated analysis actions, as in extraction and cleanup, should enhance the reproducibility, precision, and efficiency of the method. This was confirmed by validation of the overall analytical process. In the end, as a performance check, the developed method was applied on different matrices, e.g., tea, rice, grilled pork, and eel and predator eggs, as a non-food example. An inter-laboratory check was initiated as replacement for the lack of proficiency tests. Due to the high level of automation, both personnel and time effort are very low. In addition, the method is very robust with regard to the variability of the solvent selection and the loss of analytes by evaporation to dryness. It could be demonstrated that the developed method is applicable to different matrices with reproducible and precise results. This applies also to low-fat food and feed.

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An environmentally friendly method for the determination of polycyclic aromatic hydrocarbons in different soil typologies

Environmental Chemistry ◽

10.1071/en19073 ◽

2019 ◽

Vol 16 (7) ◽

pp. 517

Author(s):

Flavia De Nicola ◽

Estefanía Concha-Graña ◽

Enrica Picariello ◽

Valeria Memoli ◽

Giulia Maisto ◽

...

Keyword(s):

Polycyclic Aromatic Hydrocarbons ◽

Human Health ◽

Aromatic Hydrocarbons ◽

Analytical Method ◽

Solid Phase ◽

Environmentally Friendly ◽

Limit Of Quantification ◽

Industrial Soil ◽

Relative Standard ◽

Polycyclic Aromatic

Environmental contextPolycyclic aromatic hydrocarbons (PAHs) are widespread organic pollutants that tend to accumulate in soil. We developed an environmentally friendly analytical method for PAHs to evaluate human health risks associated with their presence in soils. The method is feasible for the analysis of soils with widely varying PAH contamination levels, and is well suited to environmental monitoring studies of relevance to human health. AbstractA microwave-assisted extraction, with a dispersive solid-phase purification step followed by programmed temperature vaporisation–gas chromatography–tandem mass spectrometry, is proposed as an environmentally friendly, simple and cheap analytical method for polycyclic aromatic hydrocarbons (PAHs) in soil. Different extraction and clean-up operating variables were tested to achieve satisfactory analytical performances: trueness from 92 to 114%, limit of quantification (LOQ) from 0.4 to 2µgkg−1 for most PAHs and intermediate precision, calculated as relative standard deviation (RSD), below 10%. The method was validated using both Certified Reference Material and real soil samples collected at sites subjected to different human activities. PAH contents ranged from 0.11 (in holm oak forest soil) to 1mgkg−1 d.w. (in an industrial soil) according to the anthropic gradient. The soil PAH contents measured were used to estimate the risk to human health, which suggested the exposure to the PAHs in soil as a potential risk for human health, especially at the industrial site. The feasibility of the method for soils with different PAH contamination degrees makes it relevant in monitoring programs.

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Liquid Chromatographic Determination of Polycyclic Aromatic Hydrocarbons in Fish and Shellfish

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/68.5.945 ◽

1985 ◽

Vol 68 (5) ◽

pp. 945-949

Author(s):

Keigo Takatsuki ◽

Shigeru Suzuki ◽

Nobutoshi Sato ◽

Isamu Ushizawa

Keyword(s):

Polycyclic Aromatic Hydrocarbons ◽

Silica Gel ◽

Aromatic Hydrocarbons ◽

Ethyl Ether ◽

Analytical Method ◽

Alkaline Digestion ◽

Polycyclic Aromatic ◽

Liquid Chromatographic ◽

Fish And Shellfish

Abstract A simple and accurate analytical method for determination of polycyclic aromatic hydrocarbons (PAHs) in fish and shellfish is presented, which is considered to be useful for routine analyses and for screening purposes. The procedure involves alkaline digestion, extraction with n-hexane, silica gel column chromatography, and liquid chromatographic (LC) determination with fluorometric detection. During development of the analytical method for determination of PAHs, it was found that benzo[a]pyrene, a representative PAH, was decomposed easily by the analytical procedure, and this tendency was investigated for the experimental conditions used. Benzo[a]pyrene was decomposed by the coexistence of alkaline conditions, light, and oxygen; by peroxides in aged ethyl ether; and by oxygen when adsorbed on silica gel. Thus, to obtain good recoveries and precise analytical results, these decomposition conditions must be avoided. The following precautions are recommended: protection from light through all analytical steps; addition of Na2S to alkaline digestion mixture as an antioxidant; complete removal of peroxides from ethyl ether just before use; quick column chromatography on silica gel; and prevention of air from contact with adsorbent. When this simple method was applied to fish and shellfish samples, very good recoveries of PAHs from fortified fish samples were obtained, and no serious interferences were observed in fish and shellfish extracts

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Simple Analytical Method for Polycyclic Aromatic Hydrocarbons [PAHs] with Fluorescence Spectrophotometer

Journal of Japan Society on Water Environment ◽

10.2965/jswe.28.249 ◽

2005 ◽

Vol 28 (4) ◽

pp. 249-255 ◽

Cited By ~ 1

Author(s):

Tasuma SUZUKI ◽

Yoshihisa SHIMIZU

Keyword(s):

Polycyclic Aromatic Hydrocarbons ◽

Aromatic Hydrocarbons ◽

Analytical Method ◽

Simple Analytical Method ◽

Polycyclic Aromatic

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Distribution and Source of Polycyclic Aromatic Hydrocarbons ( PAHs) in Soils of XinLuo District, Longyan City

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.71-78.2907 ◽

2011 ◽

Vol 71-78 ◽

pp. 2907-2911

Author(s):

Ru Ping Zhang ◽

Xia Hong Zhang ◽

Li Fang He

Keyword(s):

Polycyclic Aromatic Hydrocarbons ◽

Aromatic Hydrocarbons ◽

Analytical Method ◽

Soxhlet Extraction ◽

Industrial Area ◽

Constant Energy ◽

Synchronous Fluorescence Spectrometry ◽

Ultrasonic Assisted ◽

Polycyclic Aromatic

The analytical method for the determination of trace polycyclic aromatic hydrocarbons (PAHs) in environmental soil by ultrasonic assisted soxhlet extraction coupled with constant energy synchronous fluorescence spectrometry was described.The results indicated that were taken in Longyan City XinLuo District of soil containing 9 components: Benzo(k)fluorathene, Anthracene, Fluorathene, 1,12-benzoperylene, 2,3-benzanthracene, 1,2,5,6-dibenzo-anthracene, Fluorene, 2,3-Benzofluorene, Coronene and that the concentrations of PA Hs are in the range of 9.344~372.986 ng.g-1. Industrial area around the PAHs were relatively higher in concentration in the soil, non-industrial area around the PAHs increased with the increase of human activity increased.

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