scholarly journals Genome-wide identification and expression analysis of SABATH methyltransferases in tea plant (Camellia sinensis): insights into their roles in plant defense responses

2020 ◽  
Vol 15 (10) ◽  
pp. 1804684
Author(s):  
Yan Guo ◽  
Dahe Qiao ◽  
Chun Yang ◽  
Juan Chen ◽  
Yan Li ◽  
...  
Keyword(s):  
Plant Defense ◽  
Expression Analysis ◽  
Camellia Sinensis ◽  
Defense Responses ◽  
Tea Plant ◽  
Plant Defense Responses ◽  
Genome Wide

scholarly journals Genome-Wide Identification of the Tify Gene Family and Their Expression Profiles in Response to Biotic and Abiotic Stresses in Tea Plants (Camellia sinensis)

2020 ◽  
Vol 21 (21) ◽  
pp. 8316
Author(s):  
Xin Zhang ◽  
Wei Ran ◽  
Jin Zhang ◽  
Meng Ye ◽  
Songbo Lin ◽  
...  
Keyword(s):  
Gene Family ◽  
Camellia Sinensis ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Functional Characterization ◽  
Defense Responses ◽  
Tea Plant ◽  
Specific Gene ◽  
Mechanical Wounding ◽  
Genome Wide

The TIFY family is a plant-specific gene family that is involved in regulating a variety of plant processes, including developmental and defense responses. The chromosome-level genome of the tea plant (Camellia sinensis) has recently been released, but a comprehensive view of the TIFY family in C. sinensis (the CsTIFY genes) is lacking. The current study performed an extensive genome-wide identification of CsTIFY genes. The phylogenetics, chromosome location, exon/intron structure, and conserved domains of these genes were analyzed to characterize the members of the CsTIFY family. The expression profiles of the CsTIFY genes in four organs were analyzed, and they showed different spatial expression patterns. All CsJAZ genes were observed to be induced by jasmonate acid (JA) and exhibited different responses to abiotic and biotic stresses. Six of seven CsJAZ genes (CsJAZ1, CsJAZ2, CsJAZ3, CsJAZ4, CsJAZ7, and CsJAZ8) were upregulated by mechanical wounding and infestation with the tea geometrid (Ectropis obliqua), while infection with tea anthracnose (Colletotrichum camelliae) primarily upregulated the expression levels of CsJAZ1 and CsJAZ10. In addition, CsJAZs were observed to interact with CsMYC2 and AtMYC2. Therefore, the results of this study may contribute to the functional characterization of the CsTIFY genes, especially the members of the JAZ subfamily, as regulators of the JA-mediated defense response in tea plant.

Cloning and Expression Analysis of Auxin Efflux Carrier GeneCsPIN3in Tea Plant(Camellia sinensis)

2016 ◽  
Vol 42 (1) ◽  
pp. 58 ◽  
Author(s):  
Bo WANG ◽  
Hong-Li CAO ◽  
Yu-Ting HUANG ◽  
Yu-Rong HU ◽  
Wen-Jun QIAN ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Camellia Sinensis ◽  
Tea Plant ◽  
Cloning And Expression ◽  
Auxin Efflux Carrier

scholarly journals An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mari Kurokawa ◽  
Masataka Nakano ◽  
Nobutaka Kitahata ◽  
Kazuyuki Kuchitsu ◽  
Toshiki Furuya
Keyword(s):  
Immune Responses ◽  
Plant Defense ◽  
Pseudomonas Syringae ◽  
Large Scale ◽  
Plant Cells ◽  
Defense Responses ◽  
Root Tip ◽  
General Strategy ◽  
Plant Defense Responses ◽  
Microbe Interactions

AbstractMicroorganisms that activate plant immune responses have attracted considerable attention as potential biocontrol agents in agriculture because they could reduce agrochemical use. However, conventional methods to screen for such microorganisms using whole plants and pathogens are generally laborious and time consuming. Here, we describe a general strategy using cultured plant cells to identify microorganisms that activate plant defense responses based on plant–microbe interactions. Microbial cells were incubated with tobacco BY-2 cells, followed by treatment with cryptogein, a proteinaceous elicitor of tobacco immune responses secreted by an oomycete. Cryptogein-induced production of reactive oxygen species (ROS) in BY-2 cells served as a marker to evaluate the potential of microorganisms to activate plant defense responses. Twenty-nine bacterial strains isolated from the interior of Brassica rapa var. perviridis plants were screened, and 8 strains that enhanced cryptogein-induced ROS production in BY-2 cells were selected. Following application of these strains to the root tip of Arabidopsis seedlings, two strains, Delftia sp. BR1R-2 and Arthrobacter sp. BR2S-6, were found to induce whole-plant resistance to bacterial pathogens (Pseudomonas syringae pv. tomato DC3000 and Pectobacterium carotovora subsp. carotovora NBRC 14082). Pathogen-induced expression of plant defense-related genes (PR-1, PR-5, and PDF1.2) was enhanced by the pretreatment with strain BR1R-2. This cell–cell interaction-based platform is readily applicable to large-scale screening for microorganisms that enhance plant defense responses under various environmental conditions.

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