AbstractElectrospinning, a fabrication technique used to create non-woven, porous templates from natural and synthetic polymers, is commonly used in tissue engineering because it is highly tailorable. However, traditional electrospinning creates restrictive pore sizes that limit the required cell migration. Therefore, tissue engineering groups have sought to enhance and regulate porosity of tissue engineering templates. Air-impedance electrospinning generates templates with tailorable, patterned areas of low and high density fiber deposition. Here we demonstrate an improved air-impedance electrospinning system, consisting of a newly designed funnel equipped to hold changeable porous deposition plates and administer air flow in a confined and focused manner, with parameters that maintain template integrity. In this preliminary study, we quantify the increase in porosity of polydioxanone templates with use of traditional fiber and pore analysis as well as with mercury porosimetry. Additionally, we validate the system’s significance in fabricating enhanced porosity templates that maintain their mechanical properties (i.e. elastic modulus, tensile strength, and suture retention strength) despite the deliberate increase in porosity. This is of exceptional value to the template’s integrity and efficacy as these parameters can be further optimized to induce the desired template porosity, strength, and texture for a given application.
Fabrication and characterization of chitosan–gelatin/nanohydroxyapatite–polyaniline composite with potential application in tissue engineering scaffolds
