Fabrication and Characterization of in vitro 2D Skin Model - An attempt to establish Scaffold for Tissue Engineering

Author(s):  
Rajesh Pandiyan ◽  
Abimanyu Sugumaran ◽  
Sumathi Samiappan ◽  
Parameshwaran Sengottaiyan ◽  
Sivasankaran Ayyaru ◽  
...  
2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Daniel Aparecido Lopes Vieira da Cunha ◽  
Paulo Inforçatti Neto ◽  
Kelli Cristina Micocci ◽  
Caroline Faria Bellani ◽  
Heloisa Sobreiro Selistre-de-Araujo ◽  
...  

Scaffolds of poly(ε-caprolactone) (PCL) and their biocomposites with 0, 1, 3, and 5 wt.% Biosilicate® were fabricated by the generative manufacturing process coupled with a vertical miniscrew extrusion head to application for restoration of bone tissue. Their morphological characterization indicated the designed 0°/90° architecture range of pore sizes and their interconnectivity is feasible for tissue engineering applications. Mechanical compression tests revealed an up to 57% increase in the stiffness of the scaffold structures with the addition of 1 to 5 wt.% Biosilicate® to the biocomposite. No toxicity was detected in the scaffolds tested by in vitro cell viability with MC3T3-E1 preosteoblast cell line. The results highlighted the potential application of scaffolds fabricated with poly(ε-caprolactone)/Biosilicate® to tissue engineering.


RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 110557-110565 ◽  
Author(s):  
Yinxian Yu ◽  
Sha Hua ◽  
Mengkai Yang ◽  
Zeze Fu ◽  
Songsong Teng ◽  
...  

A composite scaffold was fabricated with a method involving both electrospinning and 3D printing to give microscale pores and good mechanical properties. Biocompatibility and cell infiltration on the scaffold was evaluated by an in vitro study.


Coatings ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 473
Author(s):  
Dilyana Gospodonova ◽  
Iliana Ivanova ◽  
Todorka Vladkova

The aim of this study was to prepare TiO2/Ag/Cu magnetron co-sputtered coatings with controlled characteristics and to correlate them with the antimicrobial activity of the coated glass samples. The elemental composition and distribution, surface morphology, wettability, surface energy and its component were estimated as the surface characteristics influencing the bioadhesion. Well expressed, specific, Ag/Cu concentration-dependent antimicrobial activity in vitro was demonstrated toward Gram-negative and Gram-positive standard test bacterial strains both by diffusion 21 assay and by Most Probable Number of surviving cells. Direct contact and eluted silver/coper nanoparticles killing were experimentally demonstrated as a mode of the antimicrobial action of the studied TiO2/Ag/Cu thin composite coatings. It is expected that they would ensure a broad spectrum bactericidal activity during the indwelling of the coated medical devices and for at least 12 h after that, with the supposition that the benefits will be over a longer time.


2009 ◽  
Vol 53 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Jessica Jean ◽  
Marc Lapointe ◽  
Jacques Soucy ◽  
Roxane Pouliot

2009 ◽  
Vol 15 (7) ◽  
pp. 1523-1532 ◽  
Author(s):  
Marc-Olivier Montjovent ◽  
Chiara Bocelli-Tyndall ◽  
Corinne Scaletta ◽  
Arnaud Scherberich ◽  
Silke Mark ◽  
...  

2014 ◽  
Vol 40 (5) ◽  
pp. 6405-6411 ◽  
Author(s):  
Abbas Teimouri ◽  
Leila Ghorbanian ◽  
Alireza Najafi Chermahini ◽  
Rahmatollah Emadi

RSC Advances ◽  
2016 ◽  
Vol 6 (107) ◽  
pp. 105125-105136 ◽  
Author(s):  
Jadi Praveen Kumar ◽  
Nandana Bhardwaj ◽  
Biman B. Mandal

Graphical abstract representing the isolation, fabrication and characterization of silk sericin/gelatin blended matrices for intended biological application.


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