Specific methanogenic activity test for anaerobic treatment of phenolic wastewater

Anaerobic treatment of high salinity sludge from marine/brackish recirculation aquaculture systems is potentially limited by inhibition of enzymatic activities and cell lysis resulting from high osmotic pressures. To further address these limitations the following investigations were conducted: effect of salinity on phosphatase activity (PA), soluble microbial products (SMP) production, and presence of extracellular polymeric substances (EPS); effect of iron (III) chloride (FeCl3) on PA and specific methanogenic activity (SMA); effect of addition of the compatible solute glycine betaine (GB) and potassium on PA, as well as on SMP and EPS production, all under saline conditions. The results show that salinity has different effects on PA of anaerobes under starvation and feeding conditions. FeCl3 increased the SMA of the sludge by 22.5% at 100 mg FeCl3/L compared with a control group (0 mg FeCl3/L). Furthermore, results of analysis of variance tests show that betaine increased the polysaccharide content of EPS and polypeptide content of SMP. However, addition of 1 mM potassium chloride did not show a significant effect on EPS and SMP composition. In conclusion, anaerobic digestion of salty sludges from a brackish aquaculture recirculation system may not be negatively affected by FeCl3 addition to concentrate waste streams, whereas GB boosts the production of SMP and EPS.

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Inhibition and anaerobic biodegradation of benz[a]anthracene-7,12- dione via the specific methanogenic activity test

International Journal of Environmental Health Engineering ◽

10.4103/2277-9183.107920 ◽

2013 ◽

Vol 2 (1) ◽

pp. 8 ◽

Cited By ~ 1

Author(s):

FazelMohammadi Moghadam ◽

MohammadMehdi Amin ◽

MehdiHajian Nezhad ◽

Marzieh Farhadkhani ◽

Mahnaz Heidari

Keyword(s):

Anaerobic Biodegradation ◽

Methanogenic Activity ◽

Activity Test ◽

Specific Methanogenic Activity

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Specific methanogenic activity test for anaerobic degradation of influents

Applied Water Science ◽

10.1007/s13201-015-0305-z ◽

2015 ◽

Vol 7 (2) ◽

pp. 535-542 ◽

Cited By ~ 21

Author(s):

Athar Hussain ◽

Shashi Kant Dubey

Keyword(s):

Anaerobic Degradation ◽

Methanogenic Activity ◽

Activity Test ◽

Specific Methanogenic Activity

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A New Methodology to Evaluate the Behaviour of Anaerobic Sludge Exposed to Potentially Inhibitory Compounds

Water Science & Technology ◽

10.2166/wst.1991.0386 ◽

1991 ◽

Vol 24 (12) ◽

pp. 201-210

Author(s):

C. A. L. Chernicharo ◽

C. M. M. Campos

Keyword(s):

Lithium Ion ◽

Anaerobic Sludge ◽

Inhibitory Effects ◽

Methanogenic Activity ◽

Activity Test ◽

Inhibitory Compounds ◽

Specific Methanogenic Activity ◽

Soluble Substrate ◽

Sludge Activity

The success of the newly developed “Specific Methanogenic Activity Test” (SMA-test) in measuring the potential of anaerobic sludge to convert soluble substrate into CH4 and CO2 (James et al., 1990) made this test potentially useful for a variety of investigations. The SMA-test was originally developed for assessment of specific methanogenic activity of anaerobic sludge, but the reliability of the method in detecting even very small changes of sludge activity has drawn attention to its use for many different purposes, especially to evaluate the behaviour of anaerobic sludge when subjected to potentially inhibitory compounds. Recent investigations into the potentially inhibitory effects of the Lithium ion on anaerobic sludge were successfully carried out employing the SMA-test. The test proved to be very sensitive for this type of experiment and the results clearly showed a relationship between the degree of inhibition and the concentration of Lithium (Anderson et al., 1991). This paper describes the methodology for measuring the specific methanogenic activity of anaerobic sludges and discusses how the SMA-test can be used for different purposes.

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Inhibition of methanogenic activity of starch-degrading granules by aromatic pollutants

Water Science & Technology ◽

10.2166/wst.1997.0320 ◽

1997 ◽

Vol 35 (8) ◽

pp. 247-253 ◽

Cited By ~ 3

Author(s):

Herbert H. P. Fang ◽

Ivan W. C. Lau ◽

Denis W. C. Chung

Keyword(s):

Chemical Industry ◽

Functional Group ◽

Anaerobic Treatment ◽

Upflow Anaerobic Sludge Blanket ◽

Uasb Reactor ◽

Anaerobic Sludge ◽

Aromatic Pollutants ◽

Methanogenic Activity ◽

Phenolic Pollutants ◽

Ic50 Values

The effects of nine common aromatic pollutants from chemical industry on the bioactivity of anaerobic granules were examined. The granules were obtained from an upflow anaerobic sludge blanket (UASB) reactor treating wastewater containing colloidal starch. The specific methanogenic activities (SMA) of granules were measured at 37°C in serum vials using 3000 mg/l of colloidal starch as substrate, plus individual pollutants at various concentrations. The toxicity was expressed by the IR50 and IC50 values, i.e. the toxicant/biomass ratio and concentration at which levels the granules exhibited only 50% of their original bioactivities. Results showed that in general the granules exhibited mild resistance to toxicity of aromatic pollutants, probably due to the granules' layered microstructure. The toxicities, which were dependent on the nature of chemical functional group, of the aromatic pollutants were in the following descending order: cresols > phenol > hydroxyphenols/phthalate > benzoate. There was only marginal difference between the toxicity of the steric isomers. For the seven phenolic pollutants, the more hydrophobic the functional group the higher the toxicity. The granules' resistance to toxicity suggested the plausibility of anaerobic treatment of wastewater from the chemical industry.

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Improving Biomethanation of Chicken Manure by Co-Digestion with Ethanol Plant Effluent

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16245023 ◽

2019 ◽

Vol 16 (24) ◽

pp. 5023 ◽

Cited By ~ 3

Author(s):

Dae-Yeol Cheong ◽

Jeffrey Todd Harvey ◽

Jinsu Kim ◽

Changsoo Lee

Keyword(s):

Loading Rate ◽

Chicken Manure ◽

Continuous Reactor ◽

Organic Loading Rate ◽

Organic Loading ◽

Methanogenic Activity ◽

Volatile Solids ◽

Methane Production Rate ◽

Specific Methanogenic Activity ◽

Ethanol Plant

As the global production of chicken manure has steadily increased, its proper management has become a challenging issue. This study examined process effluent from a bioethanol plant as a co-substrate for efficient anaerobic digestion of chicken manure. An anaerobic continuous reactor was operated in mono- and co-digestion modes by adding increasing amounts of the ethanol plant effluent (0%, 10%, and 20% (v/v) of chicken manure). Methanogenic performance improved significantly in terms of both methane production rate and yield (by up to 66% and 36%, respectively), with an increase in organic loading rate over the experimental phases. Correspondingly, the specific methanogenic activity was significantly higher in the co-digestion sludge than in the mono-digestion sludge. The reactor did not suffer any apparent process imbalance, ammonia inhibition, or nutrient limitation throughout the experiment, with the removal of volatile solids being stably maintained (56.3–58.9%). The amount of ethanol plant effluent appears to directly affect the rate of acidification, and its addition at ≥20% (v/v) to chicken manure needs to be avoided to maintain a stable pH. The overall results suggest that anerobic co-digestion with ethanol plant effluent may provide a practical means for the stable treatment and valorization of chicken manure.

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