scholarly journals Inhibition and anaerobic biodegradation of benz[a]anthracene-7,12- dione via the specific methanogenic activity test

2013 ◽  
Vol 2 (1) ◽  
pp. 8 ◽  
Author(s):  
FazelMohammadi Moghadam ◽  
MohammadMehdi Amin ◽  
MehdiHajian Nezhad ◽  
Marzieh Farhadkhani ◽  
Mahnaz Heidari
Keyword(s):  
Anaerobic Biodegradation ◽  
Methanogenic Activity ◽  
Activity Test ◽  
Specific Methanogenic Activity

Anaerobic biodegradation of 2,4,6-trichlorophenol by methanogenic granular sludge: role of co-substrates and methanogenic inhibition

2009 ◽  
Vol 59 (7) ◽  
pp. 1449-1456 ◽  
Author(s):  
D. Puyol ◽  
A. F. Mohedano ◽  
J. L. Sanz ◽  
J. J. Rodríguez
Keyword(s):  
Volatile Fatty Acids ◽  
Removal Rate ◽  
Granular Sludge ◽  
Anaerobic Biodegradation ◽  
Methanogenic Activity ◽  
The Third ◽  
Specific Methanogenic Activity ◽  
Methanol Yeast ◽  
Complete Dechlorination

The influence of several co-substrates in the anaerobic biodegradation of 2,4,6-trichlorophenol (246TCP) by methanogenic granular sludge as well as in methanogenesis inhibition by 246TCP has been studied. 4 g-COD·L−1 of lactate, sucrose, volatile fatty acids (VFA) acetate:propionate:butyrate 1:1:1, ethanol, methanol, yeast extract (YE), and 2 g-COD·L−1 of formate and methylamine were tested. Two concentrations of 246TCP: 80 mg·L−1 and 113 mg·L−1 (this last corresponding to the EC50 for acetotrophic methanogenesis) were tested. Three consecutive co-substrate and nutrient feedings were accomplished. 246TCP was added in the second feed, and the 246TCP removal rate increased considerably after the third feed. Accumulated metabolites after ortho-dechlorination, either 4-chlorophenol (4CP) (when methanol, ethanol or VFA were used as co-substrates) or 2,4-dichlorophenol (24DCP) (with lactate) avoided the complete dechlorination of 246TCP. With methylamine and formate this compound was degraded only partially. Monochlorophenols biodegradation was partially achieved with YE, but both 24DCP and 2,6-dichlorophenol (26DCP) were accumulated. In the presence of sucrose para-dechlorination was observed. 246TCP was better tolerated by methanogens when ethanol and methanol were added because of the highest specific methanogenic activity achieved with these co-substrates. Methanol and ethanol were the best co-substrates in the anaerobic biodegradation of 246TCP.

Influence of incubation conditions on the specific methanogenic activity test

2009 ◽  
Vol 21 (3) ◽  
pp. 411-424 ◽  
Author(s):  
Tatiana F. Souto ◽  
Sérgio F. Aquino ◽  
Silvana Q. Silva ◽  
Carlos A. L. Chernicharo
Keyword(s):  
Methanogenic Activity ◽  
Activity Test ◽  
Specific Methanogenic Activity

A New Methodology to Evaluate the Behaviour of Anaerobic Sludge Exposed to Potentially Inhibitory Compounds

1991 ◽  
Vol 24 (12) ◽  
pp. 201-210
Author(s):  
C. A. L. Chernicharo ◽  
C. M. M. Campos
Keyword(s):  
Lithium Ion ◽  
Anaerobic Sludge ◽  
Inhibitory Effects ◽  
Methanogenic Activity ◽  
Activity Test ◽  
Inhibitory Compounds ◽  
Specific Methanogenic Activity ◽  
Soluble Substrate ◽  
Sludge Activity

The success of the newly developed “Specific Methanogenic Activity Test” (SMA-test) in measuring the potential of anaerobic sludge to convert soluble substrate into CH4 and CO2 (James et al., 1990) made this test potentially useful for a variety of investigations. The SMA-test was originally developed for assessment of specific methanogenic activity of anaerobic sludge, but the reliability of the method in detecting even very small changes of sludge activity has drawn attention to its use for many different purposes, especially to evaluate the behaviour of anaerobic sludge when subjected to potentially inhibitory compounds. Recent investigations into the potentially inhibitory effects of the Lithium ion on anaerobic sludge were successfully carried out employing the SMA-test. The test proved to be very sensitive for this type of experiment and the results clearly showed a relationship between the degree of inhibition and the concentration of Lithium (Anderson et al., 1991). This paper describes the methodology for measuring the specific methanogenic activity of anaerobic sludges and discusses how the SMA-test can be used for different purposes.

scholarly journals Improving Biomethanation of Chicken Manure by Co-Digestion with Ethanol Plant Effluent

2019 ◽  
Vol 16 (24) ◽  
pp. 5023 ◽  
Author(s):  
Dae-Yeol Cheong ◽  
Jeffrey Todd Harvey ◽  
Jinsu Kim ◽  
Changsoo Lee
Keyword(s):  
Loading Rate ◽  
Chicken Manure ◽  
Continuous Reactor ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Methanogenic Activity ◽  
Volatile Solids ◽  
Methane Production Rate ◽  
Specific Methanogenic Activity ◽  
Ethanol Plant

As the global production of chicken manure has steadily increased, its proper management has become a challenging issue. This study examined process effluent from a bioethanol plant as a co-substrate for efficient anaerobic digestion of chicken manure. An anaerobic continuous reactor was operated in mono- and co-digestion modes by adding increasing amounts of the ethanol plant effluent (0%, 10%, and 20% (v/v) of chicken manure). Methanogenic performance improved significantly in terms of both methane production rate and yield (by up to 66% and 36%, respectively), with an increase in organic loading rate over the experimental phases. Correspondingly, the specific methanogenic activity was significantly higher in the co-digestion sludge than in the mono-digestion sludge. The reactor did not suffer any apparent process imbalance, ammonia inhibition, or nutrient limitation throughout the experiment, with the removal of volatile solids being stably maintained (56.3–58.9%). The amount of ethanol plant effluent appears to directly affect the rate of acidification, and its addition at ≥20% (v/v) to chicken manure needs to be avoided to maintain a stable pH. The overall results suggest that anerobic co-digestion with ethanol plant effluent may provide a practical means for the stable treatment and valorization of chicken manure.

Influence of the origin of the inoculum on the anaerobic biodegradability test

2004 ◽  
Vol 49 (1) ◽  
pp. 53-59 ◽  
Author(s):  
I. Moreno-Andrade ◽  
G. Buitrón
Keyword(s):  
Most Probable Number ◽  
Microbial Composition ◽  
Probable Number ◽  
Methanogenic Activity ◽  
Sulfate Reducing ◽  
Anaerobic Biodegradability ◽  
Specific Methanogenic Activity ◽  
Solids Content ◽  
Different Sources ◽  
Selection Of

Five different sources of inocula were studied to determine its influence on biodegradability tests. Inocula were characterized determining granulometry, specific methanogenic activity, solids content, and volumetric sludge index. Also, the fermentative, aceticlastic, hydrogenophilic, OPHA, and sulfate-reducing groups were determined by the most probable number technique. Anaerobic biodegradability tests were conducted with two different substrates, one easy to degrade (glucose) and a toxic one (phenol). The best performance, in terms of percent of biodegradation and lag time, for both substrates, was obtained with the inoculum from a brewery industry UASB. The results can be explained in terms of the initial activity of the inoculum. The influence of the significant variations found in the specific methanogenic activity of the five inocula studied is discussed, in terms of the microbial composition of the samples. The results emphasized the importance of the selection of an appropriate source of inoculum in order to obtain reliable results.

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