Evaluation of microbial diversity of activated sludge in a municipal wastewater treatment plant of northern China by high-throughput sequencing technology

2016 ◽  
Vol 57 (50) ◽  
pp. 23516-23521 ◽  
Author(s):  
Zhi-Bin Wang ◽  
Ming-Sheng Miao ◽  
Qiang Kong ◽  
Shou-Qing Ni
PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250514
Author(s):  
Grażyna Płaza ◽  
Łukasz Jałowiecki ◽  
Dominika Głowacka ◽  
Jakub Hubeny ◽  
Monika Harnisz ◽  
...  

Due to limited description of the role and diversity of archaea in WWTPs, the aim of the study was to analyze microbial community structures and diversities with particular regard to Archaea in the samples taken from different stages of the full-scale municipal wastewater treatment plant and effluent receiving water (upstream and downstream discharge point). Our study was focused on showing how the treatment processes influenced the Eubacteria and Archaea composition. Alpha and Beta diversity were used to evaluate the microbial diversity changes in the collected samples. Proteobacteria was the largest fraction ranging from 28% to 67% with 56% relative abundance across all samples. Archaea were present in all stages of WWTP ranged from 1 to 8%. Among the Archaea, two groups of methanogens, acetoclastic (Methanosarcina, Methanosaeta) and hydrogenotrophic methanogens (Methanospirillium, Methanoculleus, Methanobrevibacter) were dominant in the technological stages. The obtained results indicate that the treated wastewater did not significantly affect eubacterial and archaeal composition in receiving water. However, differences in richness, diversity and microbial composition of Eubacteria and Archaea between the wastewater samples taken from the primary and secondary treatment were observed.


Water ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1339
Author(s):  
Javier Bayo ◽  
Sonia Olmos ◽  
Joaquín López-Castellanos

This study investigates the removal of microplastics from wastewater in an urban wastewater treatment plant located in Southeast Spain, including an oxidation ditch, rapid sand filtration, and ultraviolet disinfection. A total of 146.73 L of wastewater samples from influent and effluent were processed, following a density separation methodology, visual classification under a stereomicroscope, and FTIR analysis for polymer identification. Microplastics proved to be 72.41% of total microparticles collected, with a global removal rate of 64.26% after the tertiary treatment and within the average retention for European WWTPs. Three different shapes were identified: i.e., microfiber (79.65%), film (11.26%), and fragment (9.09%), without the identification of microbeads despite the proximity to a plastic compounding factory. Fibers were less efficiently removed (56.16%) than particulate microplastics (90.03%), suggesting that tertiary treatments clearly discriminate between forms, and reporting a daily emission of 1.6 × 107 microplastics to the environment. Year variability in microplastic burden was cushioned at the effluent, reporting a stable performance of the sewage plant. Eight different polymer families were identified, LDPE film being the most abundant form, with 10 different colors and sizes mainly between 1–2 mm. Future efforts should be dedicated to source control, plastic waste management, improvement of legislation, and specific microplastic-targeted treatment units, especially for microfiber removal.


Proceedings ◽  
2021 ◽  
Vol 52 (1) ◽  
pp. 3
Author(s):  
Luis F. Carmo-Calado ◽  
Roberta Mota-Panizio ◽  
Gonçalo Lourinho ◽  
Octávio Alves ◽  
I. Gato ◽  
...  

The technical-economic analysis was carried out for the production of sludge-derived fuel from a municipal wastewater treatment plant (WWTP). The baseline for the analysis consists of a sludge drying plant, processing 6 m3 of sludge per day and producing a total of about 1 m3 of combustible material with 8% of moisture and a higher calorific power of 18.702 MJ/kg. The transformation of biofuel into energy translates into an electricity production of about 108 kW per 100 kg of sludge. The project in the baseline scenario demonstrated feasibility with a payback time of about six years.


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