Serum paraoxonase (a high-density lipoprotein-associated lipophilic antioxidant) activity in clinical follow-up of patients with acute pancreatitis, with particular emphasis on oxidative stress parameters and lipid profile: a prospective pilot trial

Introduction: Evidence has long existed regarding the relationship between oxidative stress and diabetes. The present study was conducted to assess the effect of atorvastatin on selected oxidative stress parameters in the form of reduced glutathione (GSH), lipid peroxidation byproduct malondialdehyde (MDA) levels, glutathione –S- transferase (GST) activity and catalase (CAT) activity) and its effect on lipid profile (total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) in dyslipidaemic type 2 diabetic patients . Materials and Methods: Fifty nine dyslipidaemic type 2 diabetic patients were included in this study. Full history was taken and general examination of patients was performed. Patients studied were taking glibenclamide (an oral hypoglycaemic drug) during the study as a treatment for their disease. These patients were followed up for 60 days and divided randomly into 2 groups. Group I (n = 31): no drug was given and served as dyslipidaemic diabetic control. Group II (n = 28): received atorvastatin tablets 20 mg once daily at night. Of the 59 Fifty patients, 46 completed the study while 13 patients withdrew. This is due to non compliance of the patients. Blood samples were drawn from the patients at the beginning and after 60 days of follow up between 8:30 & 10:30 am after at least 12-14 hours fast. Fasting blood glucose, lipid profile, selected oxidative stress parameters (GSH, MDA levels, GST and CAT activities) were measured. Renal and hepatic functions were also assessed. Results: This study revealed that: atorvastatin treatment increased serum GSH; reduced MDA levels significantly while did not significantly affect CAT and GST activity. In atorvastatin treatment, TC, TG, LDL and VLDL decreased significantly while HDL increased significantly. Conclusion: There was insignificant correlations between atorvastatin induced changes in the oxidation markers and the observed changes of the lipid profile.

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Effect of omega-3 fatty acids supplementation on cardio-metabolic and oxidative stress parameters in patients with chronic kidney disease: a systematic review and meta-analysis

BMC Nephrology ◽

10.1186/s12882-021-02351-9 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Siavash Fazelian ◽

Fatemeh Moradi ◽

Shahram Agah ◽

Akramsadat Hoseini ◽

Hafez Heydari ◽

...

Keyword(s):

Oxidative Stress ◽

Systematic Review ◽

Blood Pressure ◽

Meta Analysis ◽

Density Lipoprotein ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Oxidative Stress Parameters ◽

And Oxidative Stress ◽

Stress Parameters

Abstract Background Omega-3 fatty acids (FAs) have been suggested as a beneficial supplement in chronic kidney disease (CKD) patients, but the results of randomized clinical trials (RCTs) are controversial. We conducted a systematic review and meta-analysis to evaluate all the RCTs about the impact of omega-3 FAs supplementation on cardiometabolic outcomes and oxidative stress parameters in patients with CKD. Methods We performed a systematic database search in PubMed/MEDLINE, EMBASE, Scopus, Web of Science, and Cochrane Central, up to May 2020. We included all placebo-controlled randomized trials that assessed the effect of omega-3 FAs supplementation on any cardiometabolic outcomes: blood pressure, total cholesterol (TC), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) or triglycerides (TG) and oxidative stress parameters. Data were pooled using DerSimonian–Laird’s random-effects model. Results Finally, thirteen articles met the inclusion criteria for this review omega-3 FAs supplementation significantly decrease TC (SMD: -0.26; 95% CI: − 0.51, − 0.02; I2 = 52.7%), TG (SMD: -0.22; 95% CI: − 0.43, − 0.02; I2 = 36.0%) and Malondialdehyde (MDA) levels (SMD: -0.91; 95% CI: − 1.29, − 0.54; I2 = 00.0%) and also significantly increase superoxide dismutase (SOD) (SMD: 0.58; 95% CI: 0.27, 0.90; I2 = 00.0%) and Glutathione peroxidase (GPx) (SMD: 0.50; 95% CI: 0.14, 0.86; I2 = 00.0%) activities. However our results show that omega-3 FAs supplementation have no significant effects on HDL, LDL and blood pressure. Conclusion This systematic review and meta-analysis supports current evidence for the clinical benefit of omega-3 FAs intake to improve cardiometabolic parameters in CKD patients. However, well-designed RCTs still needed to provide a conclusive picture in this field.

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Protective and Therapeutic Effects of Orlistat on Metabolic Syndrome and Oxidative Stress in High-Fat Diet-Induced Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) in Rats: Role on Nrf2 Activation

Veterinary Sciences ◽

10.3390/vetsci8110274 ◽

2021 ◽

Vol 8 (11) ◽

pp. 274

Author(s):

Zaida Zakaria ◽

Zaidatul Akmal Othman ◽

Joseph Bagi Suleiman ◽

Nur Asyilla Che Jalil ◽

Wan Syaheedah Wan Ghazali ◽

...

Keyword(s):

Oxidative Stress ◽

Metabolic Syndrome ◽

High Fat Diet ◽

Density Lipoprotein ◽

Therapeutic Effects ◽

Metabolic Dysfunction ◽

Oxidative Stress Parameters ◽

O 10 ◽

And Oxidative Stress ◽

Stress Parameters

Metabolic dysfunction-associated fatty liver disease (MAFLD) is an excessive buildup of liver lipids closely associated with various kinds of undesirable metabolic effects and oxidative stress. We aimed to investigate the protective and therapeutic effects of orlistat on metabolic syndrome and oxidative stress parameters in high-fat diet (HFD) induced-MAFLD rats. Twenty-four male Sprague-Dawley rats were randomly divided into four groups (n = 6/group), i.e., Normal control (N), HFD, HFD + orlistat (HFD + O) (10 mg/kg/day administered concomitantly for 12 weeks as a protective model), and obese+orlistat (OB + O) (10 mg/kg/day administered 6 weeks after induction of obesity as a therapeutic model) groups. After 12 weeks, the HFD group had significantly increased Lee obesity index, serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, triglyceride, low-density lipoprotein levels, liver total cholesterol and triglyceride levels, insulin resistance and non-alcoholic steatohepatitis (NASH) together with decreased serum high-density lipoprotein level. Additionally, the HFD group also showed increased Nrf2 translocation to the nucleus with high Keap1 expression and increased liver oxidative stress parameters. Orlistat significantly improved all these alterations in HFD rats. We demonstrated that orlistat might have protective and therapeutic effects against HFD-induced MAFLD rats by its activation on Nrf2 signaling pathway, which subsequently improved metabolic syndrome and oxidative stress parameters.

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Serum Profiles of E-Selectin, Interleukin-10, and Interleukin-6 and Oxidative Stress Parameters in Patients with Acute Pancreatitis and Nonpancreatic Acute Abdominal Pain

Pancreas ◽

10.1097/00006676-200303000-00010 ◽

2003 ◽

Vol 26 (2) ◽

pp. 144-152 ◽

Cited By ~ 25

Author(s):

U. Wereszczynska-Siemiatkowska ◽

A. Dabrowski ◽

A. Siemiatkowski ◽

B. Mroczko ◽

W. Laszewicz ◽

...

Keyword(s):

Oxidative Stress ◽

Acute Pancreatitis ◽

Abdominal Pain ◽

Interleukin 6 ◽

Acute Abdominal Pain ◽

Interleukin 10 ◽

Oxidative Stress Parameters ◽

And Oxidative Stress ◽

Stress Parameters

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Effects of altered thyroid states on oxidative stress parameters in rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2015-0113 ◽

2017 ◽

Vol 28 (2) ◽

Cited By ~ 7

Author(s):

Sehkar Oktay ◽

Lebriz Uslu ◽

Nesrin Emekli

Keyword(s):

Oxidative Stress ◽

Thyroid Hormones ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Ldl Oxidation ◽

Control Group ◽

Albino Rats ◽

Oxidative Stress Parameters ◽

Altered Thyroid ◽

Stress Parameters

AbstractBackground:Thyroid hormones are effective on oxidant-antioxidant balance by leading basal metabolic rate. In this study, the effects of altered thyroid states on low density lipoprotein (LDL) oxidation and oxidative stress parameters were investigated in an experimental animal model.Methods:Thirty female Wistar Albino rats were equally divided into 3 groups as follows: control group; hypothyroid group (methimazole (75 mg/100 g was added to diet); hyperthyroid group [Results:A significant increase in lipid parameters was observed in hypothyroid group, whereas these parameters were decreased in hyperthyroid group compared to control group. For ox-LDL levels, a significant increase was observed both in hypothyroid and hyperthyroid groups. In brain, liver and kidney tissues, LPO and SA levels were increased, whereas GSH levels were decreased both in hypothyroid and hyperthyroid groups. The SOD and CAT activities were significantly decreased in hypothyroid group, however, they were increased in hyperthyroid group compared to control group. Both hypothyroid and hyperthyroid conditions modify the oxidant-antioxidant state in serum and tissues.Conclusions:Increased SOD and CAT activities in hyperthyroid group suggest that elevated thyroid hormones can reduce oxidative stress by maintaining antioxidant defense and they might have a protective effect on some tissues against oxidants.

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Intermittent fasting decreases oxidative stress parameters in Wistar rats (Rattus norvegicus)

Zinc supplementation improves heme biosynthesis in rats exposed to lead ◽

10.18051/univmed.2018.v37.31-38 ◽

2018 ◽

Vol 37 (1) ◽

pp. 31 ◽

Cited By ~ 1

Author(s):

Titis Nurmasitoh ◽

Shindy Yudha Utami ◽

Endah Kusumawardani ◽

Abdulhalim Ahmad Najmuddin ◽

Ika Fidianingsih

Keyword(s):

Oxidative Stress ◽

Lipid Profile ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Density Lipoprotein ◽

Control Group ◽

Intermittent Fasting ◽

Degenerative Diseases ◽

Oxidative Stress Parameters ◽

Stress Parameters

Background Chronic and degenerative diseases are closely related to modern lifestyles that tend to be deficient in physical activity but excessive in food intake. One method used to overcome this problem is dietary restriction through intermittent fasting. Intermittent fasting decreases the risk of chronic and degenerative diseases, e.g. by lowering oxidative stress. Oxidative stress can be determined from the malondialdehyde (MDA) levels and lipid profile in the blood. The present study aimed to determine the effect of intermittent fasting on plasma MDA levels and lipid profile of Wistar rats (Rattus norvegicus). Methods This research was a laboratory experimental research using a post test control group design. Twenty four male Wistar rats (Rattus norvegicus) were randomly assigned to 4 groups, ie control group (C), fasting group (F), non-fasting obese group (NFO), and fasting obese group (FO). Fasting treatment given in this research was intermittent fasting, comprising one day of fasting for 12 hours alternating with one day of normal feed ad libitum in the span of 8 weeks. After completion of treatment, blood was taken transcardially for examination of MDA levels and lipid profiles using spectrophotometry. Results There were significant between-group differences in MDA levels and lipid profiles (p<0.05 for all parameters). Groups treated with intermittent fasting had lower levels of MDA, total cholesterol, triglycerides, and ow-density lipoprotein (LDL) than those without intermittent fasting. The high-density lipoprotein (HDL) levels were higher in the intermittent fasting group than the other groups. Conclusion Intermittent fasting has an effect on the oxidative stress parameters of Wistar rats (Rattus norvegicus).

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