As reason of increasing requirements of thermal insulation properties of the exposed envelope, reflective insulation are among kind of modern thermal insulation materials ones, which can significantly increased thermal insulation performance if their application is correct. Thermal insulation performance is caused by reduction of the heat transfer by radiation. Conjunction of low-emissivity surface of the reflective insulation and air layer is important to account. This article is focused on the determination of thermal properties of reflective insulation and the results of measured values thermal resistance of determination by hot box method using heat flow meter are presented here. As part of determination of thermal properties of reflective insulation by experimental methods, the test element used to measure the thermal resistance of an insulated air cavity had to be created for concordance with the normative requirements. The part of this article is determination of the thermal resistance by the calculation of derived physical relations and comparison with values of the laboratory measurement.