Diversification and colonization processes in Gobioidei predicted based on mitochondrial 12S rRNA with focusing on Oxudercidae

Abstract Species of hermit crabs in the genus Clibanarius Dana, 1852 have adapted to various environments in the intertidal areas, including hard substrates and soft sediments. These species often bear a close morphological resemblance to each other, therefore, the colouration on the pereopods can be one of the reliable characteristics to distinguish the species. However, the evolutionary relationships among species with different colour patterns and relationships between colour patterns and habitat adaptation have not previously been investigated. Therefore, we reconstructed the phylogenetic relationships among 19 species of Clibanarius based on mitochondrial [12S rRNA, 16S rRNA and cytochrome oxidase I] and nuclear [histone H3] DNA markers. The results suggest that the striped and solid colour elements have evolved multiple times independently, with the ancestral colour pattern potentially being scattered, bright colour spots with a bright colour band. Our findings also suggest that evolutionary adaptation from hard substrates to mudflats and soft sediments may have occurred at least twice.

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Crenosoma striatum in lungs of European hedgehogs (Erinaceus europeus) from Portugal

Helminthologia ◽

10.2478/helm-2020-0020 ◽

2020 ◽

Vol 57 (2) ◽

pp. 179-184

Author(s):

P. F. Barradas ◽

A. R. Flores ◽

T. L. Mateus ◽

F. Carvalho ◽

F. Gärtner ◽

...

Keyword(s):

Molecular Characterization ◽

18S Rrna ◽

Population Sample ◽

Genetic Data ◽

Rrna Genes ◽

12S Rrna ◽

Rrna Gene ◽

Mitochondrial 12S Rrna ◽

18S Rrna Genes

SummaryCrenosoma striatum is a host-specifi c metastrongiloid nematode causing respiratory tract disease in hedgehogs (Erinaceus europaeus). Since few studies have reported C. striatum in hedgehogs and little genetic data is available concerning this lungworm, this study aimed to determine the occurrence of C. striatum in a population sample of hedgehogs from Portugal, additionally providing morphological, histological and molecular data. From 2017 to 2018 a survey of infection was carried out in 11 necropsied hedgehogs. Worms were extracted from fresh lung tissues and microscopically evaluated. Molecular characterization of partial mitochondrial (12S rRNA) and nuclear (18S rRNA) genes was performed. The presence of lungworms in pulmonary tissues of five hedgehogs (45.5%) was detected. Morphological and histopathological analyses evidenced adult forms of nematodes consistent with C. striatum. Molecular characterization of 18S rRNA genes confirmed the classifi cation as C. striatum. Also, novel genetic data characterizing the mitochondrial (12S rRNA) gene of C. striatum is presented.This is the first report of C. striatum infection in hedgehogs of Portugal. The findings here reported provide new insights regarding the geographic distribution and the molecular identification of this lungworm species.

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Case Report: An Eyelid Nodule Caused by Candidatus Dirofilaria hongkongensis Diagnosed by Mitochondrial 12S rRNA Sequence

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh-21-0800 ◽

2021 ◽

Author(s):

Suppapong Tirakunwichcha ◽

Lalana Sansopha ◽

Chaturong Putaporntip ◽

Somchai Jongwutiwes

Keyword(s):

Case Report ◽

Histological Examination ◽

External Surface ◽

Histopathological Examination ◽

Dirofilaria Repens ◽

12S Rrna ◽

Rrna Sequence ◽

Immature Female ◽

Upper Eyelid ◽

Mitochondrial 12S Rrna

A 59-year-old female living in Rayong Province, eastern Thailand, presented with painless, right upper eyelid nodule for 3 months. Upon removal of the eyelid mass, a well-circumscribed, firm globular mass with diameter about 1 cm was found. Histopathological examination revealed an immature female dirofilarial worm reminiscent of Dirofilaria repens, characterized by prominent sharp longitudinal ridges at external surface of the cuticle. Analysis of the mitochondrial 12S rRNA sequence showed that the worm belongs to Candidatus Dirofilaria hongkongensis. It is likely that some infections previously reported as D. repens based on histological examination may have actually been due to Candidatus D. hongkongensis.

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Reassessment of the mitochondrial 12S-rRNA gene for DNA barcoding of museum specimens of shelled marine gastropods from Japan

E3S Web of Conferences ◽

10.1051/e3sconf/202132201028 ◽

2021 ◽

Vol 322 ◽

pp. 01028

Author(s):

Nao Fukunaga ◽

Moe Shimizu ◽

Shinnosuke Teruya ◽

Nazifa Naziha Razali ◽

Satoko Nakashima ◽

...

Keyword(s):

Dna Barcoding ◽

12S Rrna ◽

Rrna Gene ◽

Museum Specimens ◽

Biodiversity Monitoring ◽

Marine Gastropods ◽

12S Rrna Gene ◽

Reference Databases ◽

Mitochondrial 12S Rrna ◽

Comprehensive Reference

DNA barcoding is an effective and powerful tool for taxonomic identification and thus very useful for biodiversity monitoring. This study investigated the usefulness of the mitochondrial 12S-rRNA gene for the DNA barcoding of shelled marine gastropods. To do so, we determined partial 12S-rRNA sequences of 75 vouchered museum specimens from 69 species of shelled gastropods from Japan. The specimens have been identified morphologically, and natural history data catalog. Sequence analyses through BLAST searches, maximum likelihood phylogenetic analysis, and species delimitation analysis suggested that the 12S-rRNA gene is helpful for barcoding shelled marine gastropods. They thus could be helpful to complement barcoding studies using other markers such as COI. The analyses successfully confirmed all samples’ identity at higher taxonomy (subfamily and above), but much less so at the species level. Our result thus also underlines the lingering problem of DNA barcoding: The lack of comprehensive reference databases of sequences. However, since we provided sequences of properly curated, vouchered museum specimens in this study, our result reported here has thus also helped to give taxonomically reliable reference sequences for biodiversity monitoring and identifications of shelled gastropods which include many important fisheries species.

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Whole mitochondrial genome screening in two families with hearing loss: detection of a novel mutation in the 12S rRNA gene

Bioscience Reports ◽

10.1042/bsr20090120 ◽

2010 ◽

Vol 30 (6) ◽

pp. 405-411 ◽

Cited By ~ 4

Author(s):

Emna Mkaouar-Rebai ◽

Nourhene Fendri-Kriaa ◽

Nacim Louhichi ◽

Abdelaziz Tlili ◽

Chahnez Triki ◽

...

Keyword(s):

Hearing Loss ◽

Mitochondrial Genome ◽

Novel Mutation ◽

Hot Spot ◽

12S Rrna ◽

Rrna Gene ◽

12S Rrna Gene ◽

Genome Screening ◽

Mitochondrial 12S Rrna ◽

Syndromic Hearing Loss

Sensorineural hearing loss has been described in association with different mitochondrial multisystemic syndromes, often characterized by an important neuromuscular involvement. Until now, mutations in mitochondrial DNA, especially in the 12S rRNA, the tRNASer(UCN) and the tRNALeu(UUR) genes, were implicated in syndromic or non-syndromic hearing loss either as a primary cause or as predisposing factors. In the present study, we performed a whole mitochondrial genome screening in two unrelated Tunisian families with inherited hearing loss. Results showed the presence of a novel mutation in the mitochondrial 12S rRNA gene in the two probands of these two families who belong to two different haplogroups: L3 and H6a1. The m.735A>G mutation affects a conserved nucleotide of the mitochondrial 12S rRNA gene in primates and other species and had a conservation index of 78.5% (11/14). We also detected known polymorphisms and sic novel mitochondrial variants. The present study confirmed that the mitochondrial 12S rRNA gene is a hot spot for mutations associated with hearing impairment.

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