EVALUATION OF THE TOXICITY OF IMIDACLOPRID IN WILD BIRDS. A NEW HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC) METHOD FOR THE ANALYSIS OF LIVER AND CROP SAMPLES IN SUSPECTED POISONING CASES

1999 ◽  
Vol 22 (10) ◽  
pp. 1547-1559 ◽  
Author(s):  
Philippe J. Berny ◽  
Florence Buronfosse ◽  
Bernadette Videmann ◽  
Thierry Buronfosse
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Wild Birds ◽  
Layer Chromatography ◽  
Hptlc Method

scholarly journals DETERMINATION OF 10-GINGEROL IN INDIAN GINGER BY VALIDATED HPTLC METHOD OF SAMPLES COLLECTED ACROSS SUBCONTINENT OF INDIA

2016 ◽  
Vol 8 (12) ◽  
pp. 190
Author(s):  
Kamran Ashraf ◽  
Syed Adnan Ali Shah ◽  
Mohd Mujeeb
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Aluminum Plates ◽  
Layer Chromatography ◽  
Hptlc Method

<p><strong>Objective: </strong>A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as perICH guidelines.</p><p><strong>Methods: </strong>The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F<sub>254</sub> using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm.</p><p><strong>Results: </strong>This system was found to have a compact spot of 10-gingerol at <em>R</em><sub>F</sub> value of 0.57±0.03. For the proposed procedure, linearity (<em>r</em><sup>2</sup> = 0.998±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%–100.68%), were found to be satisfactory.</p><p><strong>Conclusion: </strong>Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India.</p>

DEVELOPMENT OF HPTLC METHOD FOR THE ESTIMATION OF ONDANSETRON AND RANITIDINE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (12) ◽  
pp. 42-48
Author(s):  
P. J. Patel ◽  
◽  
D. A Shah ◽  
F. A. Mehta ◽  
U. K. Chhalotiya
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
High Performance ◽  
Dosage Form ◽  
Solvent System ◽  
Rf Values ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, sensitive and precise high performance thin layer chromatographic (HPTLC)method has been developed for the estimation of ondansetron (OND) and ranitidine (RAN) in combination. The method was employed on thin layer chromatography (TLC) and aluminium plates were precoated with silica gel 60 F254 as the stationary phase, while the solvent system was methanol. The Rf values were observed to be 0.5 ± 0.02, and 0.3 ± 0.02 for OND and RAN, respectively. The separated spots were densitometrically analyzed in absorbance mode at 299 nm. This method was linear in the range of 25-300 ng/band for OND and 50-600 ng/band for RAN. The limits of detection for OND and RAN were found to be 3.47 and 1.83 ng/band, respectively. The limits of quantification for OND and RAN were found to be 10.53 and 5.55 ng/band, respectively. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The method was successfully applied to the estimation of OND and RAN in combined dosage form.

scholarly journals Simultaneous Determination of Losartan and Hydrochlorothiazide in Combined Dosage Forms by First-Derivative Spectroscopy and High-Performance Thin-Layer Chromatography

2001 ◽  
Vol 84 (6) ◽  
pp. 1715-1723 ◽  
Author(s):  
Shailesh A Shah ◽  
Ishwarsinh S Rathod ◽  
Bhanubhai N Suhagia ◽  
Shrinivas S Savale ◽  
Jignesh B Patel
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Correlation Coefficients ◽  
Tolerability Profile ◽  
Angiotensin Ii Receptor ◽  
First Derivative ◽  
Derivative Spectroscopy ◽  
Layer Chromatography ◽  
Hptlc Method

Abstract Losartan (LST) is the first orally active nonpeptide angiotensin-II receptor antagonist with an improved safety and tolerability profile. It is prescribed alone or in combination with hydrochlorothiazide (HCTZ) for the treatment of moderate-to-severe hypertension. This paper describes the development of 2 methods that use different techniques, first-derivative spectroscopy and high-performance thin-layer chromatography (HPTLC), to determine LST and HCTZ in the presence of each other. LST and HCTZ in combined preparations were quantitated by using the first-derivative responses at 271.6 nm for LST and 335.0 nm for HCTZ in spectra of their solutions in water. The linearity ranges are 30–70 μg/mL for LST and 7.5–17.5 μg/mL for HCTZ with correlation coefficients of 0.9998 and 0.9997, respectively. In the HPTLC method, a mobile phase of chloroform–methanol–acetone–formic acid (7.5 + 1.5 + 0.5 + 0.03, v/v) and a prewashed Silica Gel G60 F254 TLC plate as the stationary phase were used to resolve LST and HCTZ in a mixture. Two well-separated and sharp peaks for LST and HCTZ were obtained at Rf values of 0.61 ± 0.02 and 0.41 ± 0.02, respectively. LST and HCTZ were quantitated at 254.0 nm. The linearity ranges obtained for the HPTLC method are 400–1200 and 100–300 ng/spot with corresponding correlation coefficients of 0.9944 and 0.9979, for LST and HCTZ, respectively. Both methods were validated, and the results were compared statistically. They were found to be accurate, specific, and reproducible. The methods were successfully applied to the estimation of LST and HCTZ in combined tablet formulations.

Radial "high-performance" thin-layer chromatography used to assess fetal lung maturity

1990 ◽  
Vol 36 (5) ◽  
pp. 728-731 ◽  
Author(s):  
J R Mackenzie ◽  
M Truesdale
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Fetal Lung ◽  
Additional Method ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Lung Maturity

Abstract A radial "high-performance" thin-layer chromatographic (HPTLC) method is described by which the percentages and ratios of phosphatidylinositol, sphingomyelin, lecithin, phosphatidylethanolamine, phosphatidylglycerol, and dimethyl phosphatidylethanolamine may be determined simultaneously. An additional method for radial HPTLC determination of saturated phosphatidylcholine is described. We report results of application of these methods to greater than 2000 specimens of amniotic fluid from both diabetic and nondiabetic cases.

scholarly journals Development of High-performance Thin-layer Chromatography (HPTLC) Validated Method for Simultaneous Quantification of Eucalyptol and α-Pinene in Lamiaceae Plants

2019 ◽  
pp. 1-11 ◽  
Author(s):  
Ahmed Ibrahim Foudah ◽  
Prawez Alam ◽  
Aftab Alam ◽  
Mohammed Ayman Salkini ◽  
Mohammed Hamed Alqarni ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Chromatographic Technique ◽  
Microbial Infections ◽  
Traditional Preparation ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Ich Guideline

Aim and Objective: Several plants from Lamiaceae family are used in the Saudi Arabia as a condiment and food preparation, and are generally used in the traditional preparation to treat various diseases, including anti-inflammatory, antioxidant, and microbial infections. Some of Lamiaceae species such as Mentha longifolia, Rosemarinus officinalis and Salvia officinalis having pharmacological active compounds such as α-pinene and eucalyptol. The aim of present study was to develop an accurate and precise chromatographic technique for quantification of α-pinene and eucalyptol in the Lamiaceae plants.  Methods: The high-performance thin layer chromatography (HPTLC) method was developed as per International Conference on Harmonization (ICH) guideline. Results: Simultaneous determination of α-pinene and eucalyptol was achieved by developing a densitometric analysis of high-performance thin layer chromatography (HPTLC). Silica gel 60 F254 glass-backed plates (E-Merck, Germany, 0.2 mm layers) as stationary phase and mixture n-hexane: ethyl acetate 8 : 2 (%, v/v) as mobile phase were used to produce a sharp, symmetrical and well-resolved peak at an Rf value of 0.19 ± 0.02 and 0.52 ± 0.04 for α-pinene and eucalyptol, respectively. Linearly range for α-pinene was 100–700 ng/spot (r2 = 0.9988), whereas that for eucalyptol was 1000–7000 ng/spot (r2 = 0.9987). Conclusion: The developed method was found to be a simple, accurate, and precise, and it may be used to simultaneously analyses of many medicinal plants samples containing α-pinene and eucalyptol.

scholarly journals Ecofriendly densitometric RP-HPTLC method for determination of rivaroxaban in nanoparticle formulations using green solvents

RSC Advances ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 2133-2140 ◽  
Author(s):  
Prawez Alam ◽  
Essam Ezzeldin ◽  
Muzaffar Iqbal ◽  
Md. Khalid Anwer ◽  
Gamal A. E. Mostafa ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Reversed Phase ◽  
Literature Survey ◽  
Green Solvents ◽  
Layer Chromatography ◽  
Hptlc Method

A literature survey revealed no suitable “reversed phase-high performance thin layer chromatography (RP-HPTLC)” method for the analysis of rivaroxaban in nanoparticle (NP) formulations.

scholarly journals DENSITOMETRIC VALIDATION OF LAPACHOL IN TECOMELLA UNDULATA SEEM BARK BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

2018 ◽  
Vol 10 (7) ◽  
pp. 87
Author(s):  
Raj Richa ◽  
Siddiqui Nahida ◽  
Aeri Vidhu
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Quantitative Estimation ◽  
Marker Compound ◽  
Soxhlet Apparatus ◽  
Peak Areas ◽  
Tecomella Undulata ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: To develop a validated high-performance thin layer chromatography (HPTLC) method for the quantitative estimation of Lapachol in a Soxhlet extracted bark of Tecomella undulata seem (T. undulata). Methods: The bark of T. undulata was extracted with chloroform by Soxhlet apparatus. The separation was achieved on a silica-gel 60 F254 HPTLC plate using toluene-ethyl acetate–glacial acetic acid (8.5:1.5:0.02 v/v/v) as a mobile phase. Densitometric analysis of Lapachol was carried out in absorbance mode at 254 nm.Results: The proposed method was accurate for the separation and resolution between peaks of the standard and Lapachol (5.04µg) with Rf value 0.77. Calibration curves were found to be linear over the concentration range (10-130µg) for Lapachol and correlation coefficient over (R2= 0.9973), indicating an excellent correlation between peak areas and concentrations of the marker compound. The experimentally derived LOD and LOQ for Lapachol were determined to be 0.028 µg and 0.086 µg respectively and the developed HPTLC-UV method showed lower %RSD and SEM, value indicating the method to be precise, accurate and robust.Conclusion: The study concludes that HPTLC-UV validation method can be very efficient and promising technique for the identification and quantitative analysis of Lapachol from T. undulata bark. The statistical analysis of data indicates that the developed method is reproducible and specific.

scholarly journals Determination of Active Ingredient in Synthetic Pyrethroid Formulations by High-Performance Thin-Layer Chromatography/Densitometry

2002 ◽  
Vol 85 (6) ◽  
pp. 1420-1424 ◽  
Author(s):  
Krishan K Sharma
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Synthetic Pyrethroid ◽  
Gas Liquid Chromatography ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Ec Formulations

Abstract A new, simple, precise, and rapid high-performance thin-layer chromatography (HPTLC) method was developed for the analysis of commercial emulsifiable concentrate (EC) formulations of cypermethrin, α-cypermethrin, and λ-cyhalothrin. A known amount of reference grade synthetic pyrethroid and its EC formulation was subjected to TLC, and the amount of pesticide present in the active ingredient spot was estimated by densitometry in a single beam, single wavelength reflectance mode. Calibration curves of the synthetic pyrethroid was linear in the range of 8–24 μg, and the correlation coefficient for the calibration equation ranged between 0.97 and 0.99. Recoveries from laboratory-prepared test samples of the EC formulation were in the range of 95–99%. Pesticide formulations were further analyzed by gas liquid chromatography using flame ionization detector, and results were comparable. The proposed HPTLC method has application for quality control and determination of the shelf life of EC formulations.

scholarly journals A High-Performance Thin Layer Chromatography (HPTLC) Method for Simultaneous Determination of Diphenhydramine Hydrochloride and Naproxen Sodium in Tablets

2015 ◽  
Vol 10 ◽  
pp. ACI.S31506 ◽  
Author(s):  
R.P. Bhole ◽  
S.S. Shinde ◽  
S.S. Chitlange ◽  
S.B. Wankhede
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Naproxen Sodium ◽  
Diphenhydramine Hydrochloride ◽  
Limit Of Quantitation ◽  
Layer Chromatography ◽  
Hptlc Method

A rapid and simple high-performance thin layer chromatography (HPTLC) method with densitometry at 230 nm was developed and validated for simultaneous determination of diphenhydramine hydrochloride (DPH) and naproxen sodium (NPS) from pharmaceutical preparation. The separation was carried out on aluminum plates precoated with silica gel 60 F254 using mobile phase toluene:methanol:glacial acetic acid (7.5:1:0.2, v/v/v). The linearity range lies between 200 and 1200 ng/band for DPH and 1760 and 10,560 ng/band for NPS with correlation coefficients of 0.994 and 0.995, respectively. The Rf value for DPH is 0.20 ± 0.05 and for NPS is 0.61 ± 0.06. % Recoveries of DPH and NPS was in the range of 99.70%-99.95% and 99.63%-99.95%, respectively. Limit of detection value for DPH was 13.21 ng/band and for NPS was 8.03 ng/band. Limit of quantitation value for DPH was 40.06 ng/band and for NPS was 24.34 ng/band. The developed method was validated as per ICH guidelines. In stability testing, DPH was found unstable to acid and alkaline hydrolysis, and DPH and NPS were found unstable to oxidation, whereas both the drugs were stable to neutral and photodegradation. The proposed method was successfully applied for the routine quantitative analysis of dosage form containing DPH and NPS.

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