Actin depolymerization is sufficient to induce programmed cell death in self-incompatible pollen

Self-incompatibility (SI) prevents inbreeding through specific recognition and rejection of incompatible pollen. In incompatible Papaver rhoeas pollen, SI triggers a Ca2+ signaling cascade, resulting in the inhibition of tip growth, actin depolymerization, and programmed cell death (PCD). We investigated whether actin dynamics were implicated in regulating PCD. Using the actin-stabilizing and depolymerizing drugs jasplakinolide (Jasp) and latrunculin B, we demonstrate that changes in actin filament levels or dynamics play a functional role in initiating PCD in P. rhoeas pollen, triggering a caspase-3–like activity. Significantly, SI-induced PCD in incompatible pollen was alleviated by pretreatment with Jasp. This represents the first account of a specific causal link between actin polymerization status and initiation of PCD in a plant cell and significantly advances our understanding of the mechanisms involved in SI.

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Cell Death and Metabolic Stress in Gymnodinium catenatum Induced by Allelopathy

Toxins ◽

10.3390/toxins13070506 ◽

2021 ◽

Vol 13 (7) ◽

pp. 506

Author(s):

Leyberth José Fernández-Herrera ◽

Christine Johanna Band-Schmidt ◽

Tania Zenteno-Savín ◽

Ignacio Leyva-Valencia ◽

Claudia Judith Hernández-Guerrero ◽

...

Keyword(s):

Cell Death ◽

Programmed Cell Death ◽

Protein Content ◽

Caspase 3 ◽

Cellular Stress ◽

Allelopathic Effect ◽

Sod Activity ◽

Free Media ◽

O2 Production ◽

Gymnodinium Catenatum

Allelopathy between phytoplankton species can promote cellular stress and programmed cell death (PCD). The raphidophyte Chattonella marina var. marina, and the dinoflagellates Margalefidinium polykrikoides and Gymnodinium impudicum have allelopathic effects on Gymnodinium catenatum; however, the physiological mechanisms are unknown. We evaluated whether the allelopathic effect promotes cellular stress and activates PCD in G. catenatum. Cultures of G. catenatum were exposed to cell-free media of C. marina var. marina, M. polykrikoides and G. impudicum. The mortality, superoxide radical (O2●−) production, thiobarbituric acid reactive substances (TBARS) levels, superoxide dismutase (SOD) activity, protein content, and caspase-3 activity were quantified. Mortality (between 57 and 79%) was registered in G. catenatum after exposure to cell-free media of the three species. The maximal O2●− production occurred with C. marina var. marina cell-free media. The highest TBARS levels and SOD activity in G. catenatum were recorded with cell-free media from G. impudicum. The highest protein content was recorded with cell-free media from M. polykrikoides. All cell-free media caused an increase in the activity of caspase-3. These results indicate that the allelopathic effect in G. catenatum promotes cell stress and caspase-3 activation, as a signal for the induction of programmed cell death.

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Caspase-3-like activity and proteasome degradation in grapevine suspension cell cultures undergoing silver-induced programmed cell death

Journal of Plant Physiology ◽

10.1016/j.jplph.2018.12.003 ◽

2019 ◽

Vol 233 ◽

pp. 42-51 ◽

Cited By ~ 5

Author(s):

Antonio Filippi ◽

Marco Zancani ◽

Elisa Petrussa ◽

Enrico Braidot

Keyword(s):

Cell Death ◽

Programmed Cell Death ◽

Cell Cultures ◽

Caspase 3 ◽

Suspension Cell ◽

Proteasome Degradation ◽

Suspension Cell Cultures

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Region of caspase-3 activation and programmed cell death in the early development of the mouse forebrain

Developmental Brain Research ◽

10.1016/j.devbrainres.2003.07.002 ◽

2003 ◽

Vol 145 (2) ◽

pp. 241-248 ◽

Cited By ~ 7

Author(s):

Koko Urase ◽

Yoriko Kouroku ◽

Eriko Fujita ◽

Takashi Momoi

Keyword(s):

Cell Death ◽

Programmed Cell Death ◽

Early Development ◽

Caspase 3

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Bax Inhibitor 1 (BI-1) as a conservative regulator of Programmed Cell Death

Postępy Higieny i Medycyny Doświadczalnej ◽

10.5604/01.3001.0013.6294 ◽

2019 ◽

Vol 73 ◽

pp. 681-702

Author(s):

Mirosław Godlewski ◽

Agnieszka Kobylińska

Keyword(s):

Cell Death ◽

Programmed Cell Death ◽

Actin Polymerization ◽

Functional Condition ◽

Sphingolipid Metabolism ◽

Antiapoptotic Proteins ◽

Crop Tolerance ◽

Cellular Processes ◽

C Terminus ◽

Suicidal Death

Programmed cell death (PCD) is a physiological process in which infected or unnecessary cells due to their suicidal death capability can be selectively eliminated. Pro- and antiapoptotic proteins play an important role in the induction or inhibition of this process. Presented article shows property of Bax-1 (BI-1) inhibitor which is one of the conservative protein associated with the endoplasmic reticulum (ER) as well as its cytoprotective role in the regulation of cellular processes. It was shown that: 1) BI-1 is a small protein consisting of 237 amino acids (human protein - 36 kDa) and has 6 (in animals) and 7 (in plants) α-helical transmembrane domains, 2) BI-1 is expressed in all organisms and in most tissues, moreover its level depends on the functional condition of cells and it is involved in the development or reaction to biotic and abiotic stresses, 3) BI-1 forms a pH-dependent Ca2+ channel enabling release of these ions from the ER, 4) cytoprotective effects of BI-1 requires a whole, unchanged C-terminus, 5) BI-1 can interact directly with numerous other proteins, BI-1 protein affects numerous cellular processes, including: counteracting ER stress, oxidative stress, loss of cellular Ca2+ homeostasis as well as this protein influences on sphingolipid metabolism, autophagy, actin polymerization, lysosomal activity and cell proliferation. Studies of BI-1 functions will allow understanding the mechanisms of anticancer therapy or increases the knowledge of crop tolerance to environmental stresses.

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