scholarly journals Nuclear bodies: Built to boost

2016 ◽  
Vol 213 (5) ◽  
pp. 509-511 ◽  
Author(s):  
Iain A. Sawyer ◽  
Miroslav Dundr
Keyword(s):  
Direct Evidence ◽  
Nuclear Bodies ◽  
Key Factors ◽  
Cell Biol ◽  
Histone Locus Body ◽  
Specific Reactions ◽  
Histone Locus

The classic archetypal function of nuclear bodies is to accelerate specific reactions within their crowded space. In this issue, Tatomer et al. (2016. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201504043) provide the first direct evidence that the histone locus body acts to concentrate key factors required for the proper processing of histone pre-mRNAs.

scholarly journals Distinct self-interaction domains promote Multi Sex Combs accumulation in and formation of the Drosophila histone locus body

2015 ◽  
Vol 26 (8) ◽  
pp. 1559-1574 ◽  
Author(s):  
Esteban A. Terzo ◽  
Shawn M. Lyons ◽  
John S. Poulton ◽  
Brenda R. S. Temple ◽  
William F. Marzluff ◽  
...  
Keyword(s):  
Amino Acids ◽  
Nuclear Bodies ◽  
Confocal Imaging ◽  
Histone Mrna ◽  
Mrna Accumulation ◽  
Sex Combs ◽  
Histone Locus Body ◽  
Histone Locus ◽  
Multiple Domains

Nuclear bodies (NBs) are structures that concentrate proteins, RNAs, and ribonucleoproteins that perform functions essential to gene expression. How NBs assemble is not well understood. We studied the Drosophila histone locus body (HLB), a NB that concentrates factors required for histone mRNA biosynthesis at the replication-dependent histone gene locus. We coupled biochemical analysis with confocal imaging of both fixed and live tissues to demonstrate that the Drosophila Multi Sex Combs (Mxc) protein contains multiple domains necessary for HLB assembly. An important feature of this assembly process is the self-interaction of Mxc via two conserved N-terminal domains: a LisH domain and a novel self-interaction facilitator (SIF) domain immediately downstream of the LisH domain. Molecular modeling suggests that the LisH and SIF domains directly interact, and mutation of either the LisH or the SIF domain severely impairs Mxc function in vivo, resulting in reduced histone mRNA accumulation. A region of Mxc between amino acids 721 and 1481 is also necessary for HLB assembly independent of the LisH and SIF domains. Finally, the C-terminal 195 amino acids of Mxc are required for recruiting FLASH, an essential histone mRNA-processing factor, to the HLB. We conclude that multiple domains of the Mxc protein promote HLB assembly in order to concentrate factors required for histone mRNA biosynthesis.

scholarly journals A Sequence in the Drosophila H3-H4 Promoter Triggers Histone Locus Body Assembly and Biosynthesis of Replication-Coupled Histone mRNAs

2013 ◽  
Vol 24 (6) ◽  
pp. 623-634 ◽  
Author(s):  
Harmony R. Salzler ◽  
Deirdre C. Tatomer ◽  
Pamela Y. Malek ◽  
Stephen L. McDaniel ◽  
Anna N. Orlando ◽  
...  
Keyword(s):  
Histone Mrnas ◽  
Histone Locus Body ◽  
Histone Locus

scholarly journals PML nuclear bodies and chromatin dynamics: catch me if you can!

2020 ◽  
Vol 48 (21) ◽  
pp. 11890-11912
Author(s):  
Armelle Corpet ◽  
Constance Kleijwegt ◽  
Simon Roubille ◽  
Franceline Juillard ◽  
Karine Jacquet ◽  
...  
Keyword(s):  
Nuclear Bodies ◽  
Telomere Maintenance ◽  
Physiological Mechanisms ◽  
Chromatin Dynamics ◽  
Viral Genomes ◽  
Chromatin Interactions ◽  
Pml Nuclear Bodies ◽  
Promyelocytic Leukemia Nuclear Bodies ◽  
Specific Reactions ◽  
Assembly Pathways

Abstract Eukaryotic cells compartmentalize their internal milieu in order to achieve specific reactions in time and space. This organization in distinct compartments is essential to allow subcellular processing of regulatory signals and generate specific cellular responses. In the nucleus, genetic information is packaged in the form of chromatin, an organized and repeated nucleoprotein structure that is a source of epigenetic information. In addition, cells organize the distribution of macromolecules via various membrane-less nuclear organelles, which have gathered considerable attention in the last few years. The macromolecular multiprotein complexes known as Promyelocytic Leukemia Nuclear Bodies (PML NBs) are an archetype for nuclear membrane-less organelles. Chromatin interactions with nuclear bodies are important to regulate genome function. In this review, we will focus on the dynamic interplay between PML NBs and chromatin. We report how the structure and formation of PML NBs, which may involve phase separation mechanisms, might impact their functions in the regulation of chromatin dynamics. In particular, we will discuss how PML NBs participate in the chromatinization of viral genomes, as well as in the control of specific cellular chromatin assembly pathways which govern physiological mechanisms such as senescence or telomere maintenance.

scholarly journals Drosophila Symplekin localizes dynamically to the histone locus body and tricellular junctions

Nucleus ◽  
2014 ◽  
Vol 5 (6) ◽  
pp. 613-625 ◽  
Author(s):  
Deirdre C Tatomer ◽  
Lindsay F Rizzardi ◽  
Kaitlin P Curry ◽  
Alison M Witkowski ◽  
William F Marzluff ◽  
...  
Keyword(s):  
Histone Locus Body ◽  
Histone Locus

scholarly journals Concentrating pre-mRNA processing factors in the histone locus body facilitates efficient histone mRNA biogenesis

2016 ◽  
Vol 213 (5) ◽  
pp. 557-570 ◽  
Author(s):  
Deirdre C. Tatomer ◽  
Esteban Terzo ◽  
Kaitlin P. Curry ◽  
Harmony Salzler ◽  
Ivan Sabath ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Mrna Processing ◽  
Histone Mrna ◽  
Histone Mrnas ◽  
Assembly Factor ◽  
U7 Snrnp ◽  
Histone Locus Body ◽  
Histone Locus ◽  
Processing Factors

The histone locus body (HLB) assembles at replication-dependent histone genes and concentrates factors required for histone messenger RNA (mRNA) biosynthesis. FLASH (Flice-associated huge protein) and U7 small nuclear RNP (snRNP) are HLB components that participate in 3′ processing of the nonpolyadenylated histone mRNAs by recruiting the endonuclease CPSF-73 to histone pre-mRNA. Using transgenes to complement a FLASH mutant, we show that distinct domains of FLASH involved in U7 snRNP binding, histone pre-mRNA cleavage, and HLB localization are all required for proper FLASH function in vivo. By genetically manipulating HLB composition using mutations in FLASH, mutations in the HLB assembly factor Mxc, or depletion of the variant histone H2aV, we find that failure to concentrate FLASH and/or U7 snRNP in the HLB impairs histone pre-mRNA processing. This failure results in accumulation of small amounts of polyadenylated histone mRNA and nascent read-through transcripts at the histone locus. Thus, the HLB concentrates FLASH and U7 snRNP, promoting efficient histone mRNA biosynthesis and coupling 3′ end processing with transcription termination.

scholarly journals RNA polymerase II condensate formation and association with Cajal and histone locus bodies in living human cells

2020 ◽  
Author(s):  
Takashi Imada ◽  
Takeshi Shimi ◽  
Ai Kaiho ◽  
Yasushi Saeki ◽  
Hiroshi Kimura
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Gene Clusters ◽  
Nuclear Bodies ◽  
Histone Genes ◽  
Small Nuclear Rna ◽  
Pol Ii ◽  
Histone Locus Bodies ◽  
Dynamic Structures ◽  
Histone Locus

ABSTRACTIn eukaryotic nuclei, a number of phase-separated nuclear bodies (NBs) are present. RNA polymerase II (Pol II) is the main player in transcription and forms large condensates in addition to localizing at numerous transcription foci. Cajal bodies (CBs) and histone locus bodies (HLBs) are NBs that are involved in transcriptional and post-transcriptional regulation of small nuclear RNA and histone genes. By live-cell imaging using human HCT116 cells, we here show that Pol II condensates (PCs) nucleated near CBs and HLBs, and the number of PCs increased during S phase concomitantly with the activation period of histone genes. Ternary PC–CB– HLB associates were formed via three pathways: nucleation of PCs and HLBs near CBs, interaction between preformed PC–HLBs with CBs, and nucleation of PCs near preformed CB– HLBs. Coilin knockout increased the co-localization rate between PCs and HLBs, whereas the number, nucleation timing, and phosphorylation status of PCs remained unchanged. Depletion of PCs did not affect CBs and HLBs. Treatment with 1,6-hexanediol revealed that PCs were more liquid-like than CBs and HLBs. Thus, PCs are dynamic structures often nucleated following the activation of gene clusters associated with other NBs. (187 words)

scholarly journals The Drosophila melanogaster Cajal body

2006 ◽  
Vol 172 (6) ◽  
pp. 875-884 ◽  
Author(s):  
Ji-Long Liu ◽  
Christine Murphy ◽  
Michael Buszczak ◽  
Sarah Clatterbuck ◽  
Robyn Goodman ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Motor Neurons ◽  
Nuclear Body ◽  
Nuclear Bodies ◽  
Cajal Body ◽  
Small Nuclear Rna ◽  
Nuclear Rna ◽  
Survival Of Motor Neurons ◽  
And Function ◽  
Histone Locus

Cajal bodies (CBs) are nuclear organelles that are usually identified by the marker protein p80-coilin. Because no orthologue of coilin is known in Drosophila melanogaster, we identified D. melanogaster CBs using probes for other components that are relatively diagnostic for CBs in vertebrate cells. U85 small CB–specific RNA, U2 small nuclear RNA, the survival of motor neurons protein, and fibrillarin occur together in a nuclear body that is closely associated with the nucleolus. Based on its similarity to CBs in other organisms, we refer to this structure as the D. melanogaster CB. Surprisingly, the D. melanogaster U7 small nuclear RNP resides in a separate nuclear body, which we call the histone locus body (HLB). The HLB is invariably colocalized with the histone gene locus. Thus, canonical CB components are distributed into at least two nuclear bodies in D. melanogaster. The identification of these nuclear bodies now permits a broad range of questions to be asked about CB structure and function in a genetically tractable organism.

scholarly journals Drosophila Prp40 localizes to the histone locus body and regulates gene transcription and development

2020 ◽  
Vol 133 (7) ◽  
pp. jcs239509 ◽  
Author(s):  
Silvia Prieto-Sánchez ◽  
Cristina Moreno-Castro ◽  
Cristina Hernández-Munain ◽  
Carlos Suñé
Keyword(s):  
Gene Transcription ◽  
Histone Locus Body ◽  
Histone Locus

scholarly journals The Cajal Body and Histone Locus Body

2010 ◽  
Vol 2 (7) ◽  
pp. a000653-a000653 ◽  
Author(s):  
Z. Nizami ◽  
S. Deryusheva ◽  
J. G. Gall
Keyword(s):  
Cajal Body ◽  
Histone Locus Body ◽  
Histone Locus
Sign in / Sign up

Export Citation Format

Share Document