scholarly journals THE REQUIREMENT FOR HYDROCORTISONE IN ANTIBODY-FORMING TISSUE CULTIVATED IN SERUM-FREE MEDIUM

1964 ◽  
Vol 119 (6) ◽  
pp. 1027-1049 ◽  
Author(s):  
Charles T. Ambrose
Keyword(s):  
Culture Medium ◽  
Essential Amino Acids ◽  
Secondary Response ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Secondary Antibody ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

It was previously reported from this laboratory that the secondary antibody response can regularly be elicited in vitro from fragments of rabbit lymph node node cultured in Eagle's medium supplemented with normal rabbit serum. Evidence is now presented that physiological levels of hydrocortisone (0.01 to 1.0 µM) can substitute for serum in the culture medium. However, with the omission of serum, serine (0.1 mM) must be included among Eagle's "essential" amino acids for consistent optimal antibody production. In some experiments the addition of insulin (0.5 unit/ml) and vitamin B12 (0.5 µg/ml) has further enhanced the secondary response in this serum-free medium.

Fasciola hepatica in vitro: increased susceptibility to fasciolicides in a defined serum-free medium

Parasitology ◽  
1987 ◽  
Vol 95 (1) ◽  
pp. 165-171 ◽  
Author(s):  
D. C. Jenkins ◽  
P. Topley ◽  
E. B. Rapson
Keyword(s):  
Culture Medium ◽  
Fasciola Hepatica ◽  
Liver Microsomes ◽  
Metabolic Activation ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Highly Active ◽  
Mammalian Liver

SUMMARYThe cidal properties of some phenolic, halogenated diphenyl, salicylanilide, benzimidazole and diaminophenoxyalkane anthelmintics, against 6-week-old worms of Fasciola hepatica were assessed in vitro. In a conventional fluke culture medium containing RPMI 1640, supplemented with serum with or without rabbit erythrocytes or pink-ghosts, only the halogenated diphenyl and salicylanilide compounds showed activity at concentrations equal to or less than 100 μm. However, when basal, serum and cell-free RPMI 1640 was used, all compounds other than diamphenethide were highly active, their minimum lethal concentrations being some 25–125 times lower under these conditions. The inclusion of rabbit liver microsomes in the basal culture medium resulted in diamphenethide exhibiting cidal activity equivalent to that seen when its free-amine active metabolite was assayed. The possibility that the activity of many of these compounds was masked in vitro because of their serum binding properties is discussed. Recommendations are made that in vitro screens for new fasciolicides should be carried out in serum-free medium and that additional replicates containing mammalian liver microsomes and liver cytosolic extracts be included as means for the metabolic activation of certain otherwise undetectable prodrugs.

Lipid metabolism in cultured cells. III. Cholesterol excretion process

1964 ◽  
Vol 207 (6) ◽  
pp. 1221-1225 ◽  
Author(s):  
J. Martyn Bailey
Keyword(s):  
Tissue Culture ◽  
Mammalian Cells ◽  
Cultured Cells ◽  
Rabbit Aorta ◽  
Rabbit Serum ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Cholesterol Excretion

Mammalian cells grown in tissue culture have been shown previously to take up considerable quantities of cholesterol from the growth medium. When cells grown on cholesterol-C14 supplemented medium were transferred to unlabeled medium containing serum, excretion of cholesterol into the outside medium took place. When cell cholesterol was labeled by intracellular synthesis from mevalonate-C14 precursor, it also was excreted readily into the serum medium. This excretion did not take place in serum-free medium and was found to be stimulated by a nondialyzable, thermolabile component of human serum. Horse, chicken, calf, and rabbit serum also showed stimulation ability. The process of cholesterol excretion appears to be of general occurrence. It was found in both strains of cultured cells examined (mouse fibroblasts and lymphoblasts) and also in strips of rabbit aorta incubated in vitro.

scholarly journals INHIBITION OF THE SECONDARY ANTIBODY RESPONSE IN VITRO BY SALICYLATE AND GENTISATE

1966 ◽  
Vol 124 (3) ◽  
pp. 461-482 ◽  
Author(s):  
Charles Tesch Ambrose
Keyword(s):  
Antibody Response ◽  
Sodium Salicylate ◽  
Secondary Response ◽  
Secondary Antibody ◽  
Serum Free Medium ◽  
Free Medium ◽  
Antibody Synthesis ◽  
Salicylate Concentration ◽  
Salicylic Acid Concentration

Salicylate inhibition of the secondary antibody response initiated in vitro on day 0 has been studied in cultures of rabbit lymph node fragments. Levels of 1.25 to 1.5 mM (0.20 to 0.24 mg/ml) sodium salicylate present in serum-free medium throughout an 18- or 21-day culture period completely inhibit the secondary response. This inhibition is largely accomplished by the drug's action during the first 9 days, which corresponds to the inductive phase for this culture system. Relatively little inhibition is produced by adding the drug only after day 9, although over 90% of the antibody produced during a 21-day experiment is synthesized after day 9. Studies with media of different pH's show that this inhibition is more correctly a function of the nonionized salicylic acid concentration in the medium than of the total salicylate concentration. Arguments are presented against the possibility that salicylate at the levels used here inhibits antibody synthesis by uncoupling oxidative phosphorylation. Acetylsalicylic acid (aspirin) produces the same degree of inhibition in vitro as do equimolar concentrations of sodium salicylate. Gentisate (5-hydroxysalicylate) is 15-fold more effective in producing 50% inhibition than salicylate; its temporal pattern of inhibition is similar to that of salicylate.

scholarly journals Quantification of Internalized Silica Nanoparticles via STED Microscopy

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Henrike Peuschel ◽  
Thomas Ruckelshausen ◽  
Christian Cavelius ◽  
Annette Kraegeloh
Keyword(s):  
Silica Nanoparticles ◽  
Super Resolution ◽  
Engineered Nanoparticles ◽  
Stimulated Emission ◽  
Alveolar Type ◽  
Serum Free Medium ◽  
Free Medium ◽  
Essential Information ◽  
Serum Free

The development of safe engineered nanoparticles (NPs) requires a detailed understanding of their interaction mechanisms on a cellular level. Therefore, quantification of NP internalization is crucial to predict the potential impact of intracellular NP doses, providing essential information for risk assessment as well as for drug delivery applications. In this study, the internalization of 25 nm and 85 nm silica nanoparticles (SNPs) in alveolar type II cells (A549) was quantified by application of super-resolution STED (stimulated emission depletion) microscopy. Cells were exposed to equal particle number concentrations (9.2×1010particles mL−1) of each particle size and the sedimentation of particles during exposure was taken into account. Microscopy images revealed that particles of both sizes entered the cells after 5 h incubation in serum supplemented and serum-free medium. According to thein vitrosedimentation, diffusion, and dosimetry (ISDD) model 20–27% of the particles sedimented. In comparison, 102-103NPs per cell were detected intracellularly serum-containing medium. Furthermore, in the presence of serum, no cytotoxicity was induced by the SNPs. In serum-free medium, large agglomerates of both particle sizes covered the cells whereas only high concentrations (≥ 3.8 × 1012particles mL−1) of the smaller particles induced cytotoxicity.

Hybridoma antibody production in vitro in type II serum-free medium using Nutridoma-SP supplements Comparisons with in vivo methods

1991 ◽  
Vol 145 (1-2) ◽  
pp. 213-221 ◽  
Author(s):  
Geneviève Federspiel ◽  
Kenneth C. McCullough ◽  
Ulrich Kihm
Keyword(s):  
Antibody Production ◽  
Type Ii ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

scholarly journals Cytotoxic Effects In Vitro of Highly Purified Streptolysin O on Mouse Macrophages Cultured in a Serum-Free Medium

1966 ◽  
Vol 92 (4) ◽  
pp. 1150-1153 ◽  
Author(s):  
Robert M. Fauve ◽  
Joseph E. Alouf ◽  
Albert Delaunay ◽  
Marcel Raynaud
Keyword(s):  
Serum Free Medium ◽  
Free Medium ◽  
Cytotoxic Effects ◽  
Serum Free ◽  
Streptolysin O ◽  
Mouse Macrophages

Innovative serum-free medium for in vitro cultivation of promastigote forms of Leishmania species

2008 ◽  
Vol 136 ◽  
pp. S150
Author(s):  
Abdalla Hassan Sharief ◽  
Eltahir A. Khalil ◽  
Samia A. Omer ◽  
Hamid S. Abdalla
Keyword(s):  
Leishmania Species ◽  
In Vitro Cultivation ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free
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