H-2-controlled suppression of T cell response to lactate dehydrogenase B. Characterization of the lactate dehydrogenase B suppressor pathway.

We characterized the cell types involved in the H-2-controlled suppression of T cell response to lactate dehydrogenase B (LDHB). The suppressor effector (Tse) was found to be an Lyt-1+2+, J+ cell that recognizes antigen together with Ek molecules of antigen-presenting cells (APC). To become functional, the Tse cell requires a second signal from a nonspecific, Lyt-1+2-, J+ suppressor-inducer (Tsi) cell. The Tsi-Tse interaction is not subject to any genetic restriction. The target cell of suppression is an Lyt-1+2-, J- (most likely T helper [Th]) cell that recognizes LDHB in the context of A molecules on APC. The suppression is manifested in inhibition of the antigen-specific, A-restricted proliferation of Th cells. The interaction between Tse and Th is restricted by the A region of the H-2 complex. Because this restriction is determined by the receptor of Th cells, the mechanism of Th-Tse interaction most likely involves a concomitant recognition of LDHB and A region-controlled molecules by Th cells on the surface of Tse cells.

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Human CD4+ T-cell response to hepatitis delta virus: identification of multiple epitopes and characterization of T-helper cytokine profiles.

Journal of Virology ◽

10.1128/jvi.71.3.2241-2251.1997 ◽

1997 ◽

Vol 71 (3) ◽

pp. 2241-2251 ◽

Cited By ~ 72

Author(s):

R Nisini ◽

M Paroli ◽

D Accapezzato ◽

F Bonino ◽

F Rosina ◽

...

Keyword(s):

T Cell ◽

Cell Response ◽

Hepatitis Delta Virus ◽

T Cell Response ◽

Cd4 T Cell ◽

T Helper ◽

Hepatitis Delta ◽

Virus Identification ◽

Delta Virus

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A Novel 62-Kilodalton Egg Antigen fromSchistosoma mansoni Induces a Potent CD4+ T Helper Cell Response in the C57BL/6 Mouse

Infection and Immunity ◽

10.1128/iai.67.4.1729-1735.1999 ◽

1999 ◽

Vol 67 (4) ◽

pp. 1729-1735 ◽

Cited By ~ 20

Author(s):

Hiroko Asahi ◽

Hector J. Hernandez ◽

Miguel J. Stadecker

Keyword(s):

T Cell ◽

Cell Response ◽

Phosphoenolpyruvate Carboxykinase ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

T Cell Response ◽

T Helper ◽

Th Cells ◽

Cytokine Analysis ◽

Egg Antigen

ABSTRACT In infection with Schistosoma mansoni, hepatic granuloma formation is mediated by CD4+ T helper (Th) cells sensitized to schistosomal egg antigens. There is considerable variation among infected individuals with respect to both severity of disease and the T-cell response to egg antigens. In the BL/6 mouse, the egg granulomas are relatively small and the relevant sensitizing egg antigens are largely unknown. We investigated the CD4+ Th cell response of infected BL/6 mice to egg antigens fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found a prominent lymphoproliferative response to be directed against a 62-kDa component. With the aid of a specific T-cell hybridoma, 4E6, the 62-kDa antigen was isolated; following partial digestion with endoproteinase Glu-C, an internal amino acid sequence was found to be identical with one present in the enzyme phosphoenolpyruvate carboxykinase (PEPCK) of the organisms Caenorhabditis elegans and Treponema pallidum and to differ by one residue from PEPCK of various other species. In CD4+ Th cells from 7.5- 8.5-week-infected BL/6 mice, the purified 62-kDa molecule elicited a potent proliferative response which, based on cytokine analysis, was of a mixed Th-1 and Th-2 type. Our results reveal a novel egg antigen of particular prominence in the BL/6 mouse and suggest that the immune response in schistosomiasis is a product of sensitization to egg antigens that may vary considerably in immunogenicity from strain to strain.

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