scholarly journals Interleukin 5 messenger RNA expression by eosinophils in the intestinal mucosa of patients with coeliac disease.

1992 ◽  
Vol 175 (1) ◽  
pp. 293-296 ◽  
Author(s):  
P Desreumaux ◽  
A Janin ◽  
J F Colombel ◽  
L Prin ◽  
J Plumas ◽  
...  
Keyword(s):  
Coeliac Disease ◽  
Messenger Rna ◽  
Eosinophil Infiltration ◽  
T And B Cells ◽  
Cell Infiltrate ◽  
Interleukin 5 ◽  
Mucosal Cells ◽  
Typical Morphology ◽  
Sense Probe ◽  
Blood Eosinophils

Interleukin 5 (IL-5), the major factor involved in eosinophil differentiation, is produced by T cells or mast cells. In the present study, we found that eosinophils infiltrating the mucosa of four patients with active coeliac disease also express the IL-5 mRNA. No positive signal was obtained in normal duodenum tissues and in the cell infiltrate from patients submitted to gluten restriction. The identification of labeled mucosal cells as eosinophils relied on their typical morphology. Moreover, highly purified blood eosinophils from three out of four patients with eosinophilia were also strongly labeled with the IL-5 antisense but not with the corresponding sense probe. Together, these results suggest that eosinophils have the capacity to synthesize IL-5, which could contribute to paracrine interactions with T and B cells and, in autocrine fashion, locally participate, through binding to the IL-5 receptor, to eosinophil differentiation and activation. These data might have implications not only in the pathology of coeliac disease but also in other diseases associated with eosinophil infiltration.

scholarly journals Human eosinophils synthesize and secrete interleukin-6, in vitro

Blood ◽  
1992 ◽  
Vol 80 (6) ◽  
pp. 1496-1501 ◽  
Author(s):  
Q Hamid ◽  
J Barkans ◽  
Q Meng ◽  
S Ying ◽  
JS Abrams ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Messenger Rna ◽  
Human Peripheral Blood ◽  
Immunocytochemical Staining ◽  
Ifn Gamma ◽  
Cellular Source ◽  
Sense Probe ◽  
Blood Eosinophils

Abstract Using the technique of in situ hybridization, we have shown that resting, unstimulated, human peripheral blood eosinophils, obtained from subjects with greater than 8% eosinophilia, transcribe and translate messenger RNA (mRNA) for interleukin-6 (IL-6). After incubation for 24 hours in culture medium alone, approximately 19% of eosinophils were positive for IL-6 mRNA. This may be a reflection of their in vivo activation, but also may suggest that the gene for this cytokine is constitutively expressed in eosinophils. After stimulation with interferon gamma (IFN gamma) (500 U/mL), the percentage of IL-6- mRNA+ cells increased to 51.3%. This was accompanied by an enhancement of intensity of the hybridization signals. The specificity of the IL-6 probe and the hybridization signals was confirmed by the use of an IL-6 sense probe and RNase pretreatment of cell preparations. Evidence for the translation of IL-6 mRNA was obtained by immunocytochemical staining. Normal and activated eosinophils gave IL-6-specific immunoreactivity with a polyclonal antihuman IL-6 antibody. A higher percentage of positive cells was detected among activated eosinophils than those treated with medium alone. Using a specific immunoenzymetric assay, we detected 190.15 +/- 18.1 and 403.32 +/- 213.6 pg/mL of IL-6 in supernatants of unstimulated and IFN gamma-treated (24 and 48 hours) eosinophils, respectively. These data indicate that eosinophils are an important cellular source of IL-6.

scholarly journals Human eosinophils synthesize and secrete interleukin-6, in vitro

Blood ◽  
1992 ◽  
Vol 80 (6) ◽  
pp. 1496-1501 ◽  
Author(s):  
Q Hamid ◽  
J Barkans ◽  
Q Meng ◽  
S Ying ◽  
JS Abrams ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Messenger Rna ◽  
Human Peripheral Blood ◽  
Immunocytochemical Staining ◽  
Ifn Gamma ◽  
Cellular Source ◽  
Sense Probe ◽  
Blood Eosinophils

Using the technique of in situ hybridization, we have shown that resting, unstimulated, human peripheral blood eosinophils, obtained from subjects with greater than 8% eosinophilia, transcribe and translate messenger RNA (mRNA) for interleukin-6 (IL-6). After incubation for 24 hours in culture medium alone, approximately 19% of eosinophils were positive for IL-6 mRNA. This may be a reflection of their in vivo activation, but also may suggest that the gene for this cytokine is constitutively expressed in eosinophils. After stimulation with interferon gamma (IFN gamma) (500 U/mL), the percentage of IL-6- mRNA+ cells increased to 51.3%. This was accompanied by an enhancement of intensity of the hybridization signals. The specificity of the IL-6 probe and the hybridization signals was confirmed by the use of an IL-6 sense probe and RNase pretreatment of cell preparations. Evidence for the translation of IL-6 mRNA was obtained by immunocytochemical staining. Normal and activated eosinophils gave IL-6-specific immunoreactivity with a polyclonal antihuman IL-6 antibody. A higher percentage of positive cells was detected among activated eosinophils than those treated with medium alone. Using a specific immunoenzymetric assay, we detected 190.15 +/- 18.1 and 403.32 +/- 213.6 pg/mL of IL-6 in supernatants of unstimulated and IFN gamma-treated (24 and 48 hours) eosinophils, respectively. These data indicate that eosinophils are an important cellular source of IL-6.

scholarly journals Epstein-Barr virus transformed B lymphocytes produce interleukin-5

Blood ◽  
1990 ◽  
Vol 75 (7) ◽  
pp. 1400-1403 ◽  
Author(s):  
CC Paul ◽  
JR Keller ◽  
JM Armpriester ◽  
MA Baumann
Keyword(s):  
B Cell ◽  
B Lymphocytes ◽  
Messenger Rna ◽  
Epstein Barr Virus ◽  
Northern Analysis ◽  
Interleukin 5 ◽  
Barr Virus ◽  
Epstein Barr ◽  
Semi Solid ◽  
Culture Supernatants

Abstract Interleukin-5 (IL-5) has previously been isolated only as a product of T lymphocytes. We have found that Epstein-Barr virus transformed B lymphocytes produce large amounts of IL-5 activity in culture supernatants, inducing proliferation of murine BCL1 cells, and supporting the selective growth of eosinophil colonies in semi-solid culture. Production of IL-5 messenger RNA by transformed B-cell lines was verified by Northern analysis using a 3.2-kilobase cloned DNA fragment containing the full-length human IL-5 gene, and immunoreactive IL-5 was detected in B-cell culture supernatants. These findings suggest a possible expanded role for the B cell in the induction of eosinophilia, and should serve as a focus for additional investigation into possible roles for IL-5 in human B-cell proliferation and differentiation.

Interleukin-5 messenger RNA expression in peripheral blood CD4+ cells in asthma

1996 ◽  
Vol 97 (6) ◽  
pp. 1320-1328 ◽  
Author(s):  
Christopher K.W. Lai ◽  
Alice S.S. Ho ◽  
Christopher H.S. Chan ◽  
Janet Tang ◽  
Joseph C.K. Leung ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Messenger Rna ◽  
Rna Expression ◽  
Cd4 Cells ◽  
Interleukin 5

scholarly journals The immune cell infiltrate populating meningiomas is composed of mature, antigen-experienced T and B cells

2013 ◽  
Vol 15 (11) ◽  
pp. 1479-1490 ◽  
Author(s):  
L. Fang ◽  
D. E. Lowther ◽  
M. L. Meizlish ◽  
R. C. E. Anderson ◽  
J. N. Bruce ◽  
...  
Keyword(s):  
B Cells ◽  
Immune Cell ◽  
T And B Cells ◽  
Cell Infiltrate ◽  
Immune Cell Infiltrate

Interleukin-4, interleukin-5, and granulocyte-macrophage colony-stimulating factor receptor expression in chronic sinusitis and response to topical steroids

1998 ◽  
Vol 118 (4) ◽  
pp. 490-495 ◽  
Author(s):  
Erin D. Wright ◽  
Saul Frenkiel ◽  
Khalid Al-Ghamdi ◽  
Omar Ghaffar ◽  
Peter Small ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Chronic Sinusitis ◽  
Receptor Expression ◽  
Interleukin 4 ◽  
Topical Steroids ◽  
Interleukin 5 ◽  
Gm Csf ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Interleukin 4 Receptor

Chronic sinusitis and its associated eosinophilic infiltrate are believed to be mediated, at least in part, by the upregulation of Th-2 cytokines, including interleukin-4, interleukin-5, and granulocyte-macrophage colony-stimulating factor (GM-CSF). Interleukin-4 is involved in IgE production and in eosinophil recruitment through upregulation of vascular cell adhesion molecule-1. Interleukin-5 and GM-CSF are involved in eosinophil growth and survival. The aim of this study was to investigate the expression of receptors for these cytokines in the sinus mucosa of subjects with chronic sinusitis. Using the technique of in situ hybridization to detect specific cytokine receptor messenger RNA, we studied the sinus mucosa of subjects with nonallergic chronic sinusitis, subjects with allergic chronic sinusitis, subjects with allergic chronic sinusitis treated with topical steroids, and normal controls. Our data demonstrate higher expression of interleukin-4 receptor in subjects with allergic chronic sinusitis than in controls ( p <0.001) and higher expression of interleukin-5 receptor in both subjects with nonallergic chronic sinusitis and subjects with allergic chronic sinusitis than in controls ( p <0.001, p <0.001). The expression of interleukin-4 receptor and interleukin-5 receptor was higher in subjects with allergic chronic sinusitis than in subjects with nonallergic chronic sinusitis ( p <0.001). GM-CSF receptor expression was also found to be higher in subjects with allergic chronic sinusitis and subjects with nonallergic chronic sinusitis than in controls ( p <0.001, p <0.001). In contrast to interleukin-4 receptor and interleukin-5 receptor, however, expression of GM-CSF receptor was higher in subjects with non-allergic chronic sinusitis than in subjects with allergic chronic sinusitis ( p <0.001). In subjects with allergic chronic sinusitis treated with topical corticosteroids, the expression of interleukin-4 receptor and interleukin-5 receptor messenger RNA levels was significantly lower than levels in patients with allergic chronic sinusitis who were not taking topical steroids ( p <0.001, p <0.001). Steroid treatment had no effect on GM-CSF receptor messenger RNA expression. In conclusion, our data support a role for Th-2 cytokine receptors in the pathophysiology of chronic sinusitis. Further, our data lend support to the theory that differential activation of distinct cytokine pathways mediates inflammation in chronic sinusitis depending on whether there is associated allergy. Finally, treatment with topical corticosteroids has been demonstrated in chronic sinusitis to downregulate receptors for interleukin-4 and interleukin-5.

Interleukin-5 Messenger RNA and Immunoreactive Protein Expression by Activated Eosinophils in Lesional Atopic Dermatitis Skin

1994 ◽  
Vol 103 (4) ◽  
pp. 589-592 ◽  
Author(s):  
Yoichi Tanaka ◽  
Emmanuel Delaporte ◽  
Sylvain Dubucquoi ◽  
Abdelilah Soussi Gounni ◽  
Eliane Porchet ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Protein Expression ◽  
Messenger Rna ◽  
Immunoreactive Protein ◽  
Interleukin 5

scholarly journals Interleukin 10 inhibits lipopolysaccharide-induced survival and cytokine production by human peripheral blood eosinophils.

1994 ◽  
Vol 180 (2) ◽  
pp. 711-715 ◽  
Author(s):  
S Takanaski ◽  
R Nonaka ◽  
Z Xing ◽  
P O'Byrne ◽  
J Dolovich ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Cytokine Production ◽  
Human Peripheral Blood ◽  
Interleukin 10 ◽  
Activation Mechanism ◽  
Eosinophil Infiltration ◽  
Dependent Manner ◽  
Gm Csf ◽  
Eosinophil Survival ◽  
Blood Eosinophils

In this study we have investigated the effects of interleukin 10 (IL-10) on human peripheral blood eosinophils stimulated with granulocyte/macrophage colony stimulating factor (GM-CSF) and lipopolysaccharide (LPS). We show that LPS was able to enhance eosinophil survival in a dose-dependent manner, as well as release of the cytokines GM-CSF, tumor necrosis factor alpha, and IL-8. LPS-induced eosinophil survival was largely inhibited by an anti-GM-CSF neutralizing antibody and completely blocked by polymyxin B, suggesting GM-CSF involvement in the survival enhancing mechanism and LPS specificity, respectively. IL-10 significantly inhibited survival of, and cytokine production from, eosinophils induced by LPS, but did not inhibit the survival induced by GM-CSF. These observations suggest a novel activation mechanism of eosinophils and, also, that IL-10 may participate in the regulation of diseases characterized by eosinophil infiltration.

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