Genetics of Phytopathogenic Fungi. XII. Detection of Esterases and Phosphatases in Culture Filtrates of Fusarium oxysporum and F. xylarioides by Starch-Gel Zone Electrophoresis

1964 ◽  
Vol 125 (4) ◽  
pp. 298-300 ◽  
Author(s):  
J. A. Meyer ◽  
E. D. Garber ◽  
Susan G. Shaeffer
Keyword(s):  
Fusarium Oxysporum ◽  
Phytopathogenic Fungi ◽  
Culture Filtrates ◽  
Zone Electrophoresis ◽  
Starch Gel

GENETICS OF PHYTOPATHOGENIC FUNGI: PECTOLYTIC ENZYMES OF VIRULENT AND AVIRULENT STRAINS OF THREE PHYTOPATHOGENIC PENICILLIA

1966 ◽  
Vol 44 (12) ◽  
pp. 1645-1650 ◽  
Author(s):  
E. D. Garber ◽  
L. Beraha
Keyword(s):  
Organic Carbon ◽  
Pectin Methylesterase ◽  
Defined Medium ◽  
Relative Activity ◽  
Phytopathogenic Fungi ◽  
Avirulent Strain ◽  
Culture Filtrates ◽  
Pectolytic Enzymes ◽  
Lyase Activity ◽  
Starch Gel

Culture filtrates from a virulent and an avirulent strain of each of three phytopathogenic species of Penicillium grown in a defined medium to which were added different sources of organic carbon had endopolygalacturonase (endoPG) and exopolygalacturonase (exoPG), but neither detectable pectin methylesterase nor pectin lyase activity. Extracts from diseased tissue of oranges inoculated with P. italicum or P. digitatum and from diseased tissue of apples inoculated with P. expansum, but not from healthy fruits, had endoPG and exoPG activity. Culture filtrates and extracts of diseased tissue were subjected to vertical starch-gel zone electrophoresis. The number, location (anodic, cathodic), electrophoretic mobility, and relative activity of sites of endoPG or exoPG activity were determined by the species, virulence or avirulence of the strain, source of organic carbon, and gel pH.

scholarly journals Potential antifungal effects of silver nanoparticles (AgNPs) of different sizes against phytopathogenic Fusarium oxysporum f. sp. radicis-lycopersici (FORL) strains

2021 ◽  
Vol 3 (4) ◽  
Author(s):  
Ilgin Akpinar ◽  
Muammer Unal ◽  
Taner Sar
Keyword(s):  
Silver Nanoparticles ◽  
Fusarium Oxysporum ◽  
Antifungal Agents ◽  
New Technology ◽  
Economic Loss ◽  
Phytopathogenic Fungi ◽  
Growth Media ◽  
Mycelium Growth ◽  
Antifungal Effects ◽  
Critical Problems

AbstractFusarium species are the primary fungal pathogen affecting agricultural foodstuffs both in crop yield and economic loss. Due to these problems, control of phytopathogenic fungi has become one of the critical problems around the World. Nanotechnology is a new technology with potential in many fields, including agriculture. This study focused on determining potential effects of silver nanoparticles (AgNPs) with different nanosizes (3, 5, 8 and 10 nm) and at different concentrations (12.5–100 ppm) against phytopathogenic Fusarium oxysporum f. sp. radicis-lycopersici (FORL) strains. The maximum antifungal activity was achieved by decreasing nanosize and increasing concentration of AgNPs. Mycelium growth abilities were decreased about 50%, 75% and 90% by AgNPs treatment with 3 nm sizes at 25 ppm, 37.5 ppm and 50 ppm concentrations, respectively. The productivity of fungal biomass in the liquid growth media was found to be too limited at the 25–37.5 ppm of AgNPs concentrations with all sizes. In addition, both septation number and dimensions of micro- and macroconidia were found to be gradually decreased with the application of silver nanoparticles. This work showed that the low concentration of AgNPs could be used as potential antifungal agents and applied for control of phytopathogens.

Antifungal Activities of Some Indole Derivatives

2010 ◽  
Vol 65 (7-8) ◽  
pp. 437-439 ◽  
Author(s):  
Hui Xu ◽  
Qin Wang ◽  
Wen-Bin Yang
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Graminearum ◽  
Broad Spectrum ◽  
Alternaria Alternata ◽  
Phytopathogenic Fungi ◽  
Pyricularia Oryzae ◽  
Indole Derivatives ◽  
Alternaria Brassicae ◽  
Antifungal Activities

Nine indole derivatives were evaluated in vitro against Fusarium graminearum, Alternaria alternata, Helminthosporium sorokinianum, Pyricularia oryzae, Fusarium oxysporum f. sp. vasinfectum, Fusarium oxysporum f. sp. cucumarinum, and Alternaria brassicae. Most of the compounds were found to possess antifungal activities. Especially compounds 2, 5, 8, and 9 exhibited broad-spectrum antifungal activities against the above-mentioned seven phytopathogenic fungi, and showed more potent activities than hymexazole, a commercial agricultural fungicide.

Purification and Characterization of Two Proteins from Culture Filtrates of Mycobacterium tuberculosis H 37 Ra Strain

1970 ◽  
Vol 1 (2) ◽  
pp. 164-168
Author(s):  
Thomas M. Daniel ◽  
Lavenia E. Ferguson
Keyword(s):  
Molecular Weight ◽  
Mycobacterium Tuberculosis ◽  
Skin Testing ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Purification And Characterization ◽  
Culture Filtrates ◽  
Zone Electrophoresis

Two proteins have been purified from culture filtrates of Mycobacterium tuberculosis , H 37 Ra strain by a procedure combining gel filtration, diethylaminoethyl (DEAE)-cellulose chromatography, and zone electrophoresis. The two proteins are similar in molecular weight but differ slightly in charge. The faster migrating protein, designated a 1 , is not antigenic. The slower migrating protein, designated a 2 , is antigenic both with respect to antisera and as a skin-testing antigen.

PROPERTIES AND CLASSIFICATION OF THE SOLUBLE ESTERASES OF HUMAN BRAIN

1966 ◽  
Vol 44 (2) ◽  
pp. 225-232 ◽  
Author(s):  
D. J. Ecobichon
Keyword(s):  
Alkaline Phosphatase ◽  
Human Brain ◽  
Esterase Activity ◽  
Water Soluble ◽  
Human Tissues ◽  
Esterase Pattern ◽  
Vertical Zone ◽  
Zone Electrophoresis ◽  
Starch Gel

Water-soluble proteins and enzymes of human brain were separated by vertical zone electrophoresis in starch gel. Fifteen bands of esterase activity were detected in brain. Various substrates and inhibitors were used in efforts to identify enzymes in addition to a comparison of the esterase pattern with patterns obtained from other human tissues. One zone, composed of four bands of acetylesterase activity, was found to be common to all the tissues investigated with the exception of serum. Two bands of cholinesterase and two bands of A-esterase activity were identified. The remaining bands, which were aliesterases possessing broad overlapping substrate specificity and inhibitor sensitivity, were electrophoretically different from those of other tissues. Observations on alkaline phosphatase, acid phosphatase, and lactate dehydrogenase were recorded for comparison with the data on esterases.

Comparative zone electrophoresis of catalase of Staphylococcus species isolated from mammalian skin

1976 ◽  
Vol 22 (12) ◽  
pp. 1691-1698 ◽  
Author(s):  
Raymond J. Zimmerman
Keyword(s):  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Polyacrylamide Electrophoresis ◽  
Electrophoretic Mobilities ◽  
Mammalian Skin ◽  
Zone Electrophoresis ◽  
Starch Gel ◽  
Molecular Sieving

Vertical polyacrylamide slab gel electrophoresis was conducted for the catalase enzymes of representative strains of 18 proposed species and subspecies of the genus Staphylococcus. The catalase bands which resulted were predominantly monomorphic within each of the species and differences in catalase mobilities were observed between many of the species. The electrophoretic mobilities of the catalases were supportive to the scheme of classification used. Many strains of certain species demonstrated multiple catalase bands which are suggestive of multimolecular forms of the enzyme. Horizontal starch gel electrophoresis of representative strains of S. capitis produced catalase bands with relative mobilities that were different from those obtained with polyacrylamide electrophoresis, presumably due to a difference in molecular sieving between the gels.

Synthesis and Antifungal Activities of Some 2,6-Bis-(Un)Substituted Phenoxymethylpyridines

2010 ◽  
Vol 65 (7-8) ◽  
pp. 433-436
Author(s):  
Hui Xu ◽  
Huan Qu
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Fusarium Graminearum ◽  
Broad Spectrum ◽  
Alternaria Alternata ◽  
Phytopathogenic Fungi ◽  
Alternaria Brassicae ◽  
Antifungal Activities

Several 2,6-bis-(un)substituted phenoxymethylpyridines were synthesized and evaluated in vitro against Fusarium graminearum, Helminthosporium sorokinianum, Alternaria brassicae, Alternaria alternata, and Fusarium oxysporum f. sp. vasinfectum. Among all derivatives, compound 3 a exhibited a broad-spectrum antifungal activity against the five phytopathogenic fungi.

Effect of Buffers on the Pectolytic Activity of Culture Filtrates of Fusarium oxysporum

Nature ◽  
1964 ◽  
Vol 202 (4930) ◽  
pp. 414-415 ◽  
Author(s):  
J. A. MEYER ◽  
E. D. GARBER ◽  
SUSAN G. SHAEFFER
Keyword(s):  
Fusarium Oxysporum ◽  
Culture Filtrates ◽  
Pectolytic Activity
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